ANALYSIS, ISOLATION AND ACTIVATION OF ANTIGEN-SPECIFIC CD4+ AND CD8+ T CELLS BY SOLUBLE MHC-PEPTIDE COMPLEXES

ANALYSIS, ISOLATION AND ACTIVATION OF ANTIGEN-SPECIFIC CD4+ AND CD8+ T CELLS BY SOLUBLE MHC-PEPTIDE COMPLEXES

T cells constitute the core of adaptive cellular immunity and protect higher organisms against pathogen infections and cancer. Monitoring of disease progression as well as prophylactic or therapeutic vaccines and immunotherapies call for conclusive detection, analysis and sorting of antigen-specific...

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Bibliographic Details
Main Authors: Julien eSchmidt, Danijel eDojcinovic, Philippe eGuillaume, Immanuel eLuescher
Format: Article
Language:English
Published: Frontiers Media S.A. 2013-07-01
Series:Frontiers in Immunology
Subjects:
Flow Cytometry
Vaccination
T cells
T cell receptor
coreceptor
MHC I
Online Access:http://journal.frontiersin.org/Journal/10.3389/fimmu.2013.00218/full
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spelling doaj-56c4f850c1e542369e70c7e3e5c00c4d2020-11-24T21:20:18ZengFrontiers Media S.A.Frontiers in Immunology1664-32242013-07-01410.3389/fimmu.2013.0021857410ANALYSIS, ISOLATION AND ACTIVATION OF ANTIGEN-SPECIFIC CD4+ AND CD8+ T CELLS BY SOLUBLE MHC-PEPTIDE COMPLEXESJulien eSchmidt0Danijel eDojcinovic1Philippe eGuillaume2Immanuel eLuescher3Ludwig center for cancer resreach@University of LausanneTCMetrix sàrlTCMetrix sàrlLudwig center for cancer resreach@University of LausanneT cells constitute the core of adaptive cellular immunity and protect higher organisms against pathogen infections and cancer. Monitoring of disease progression as well as prophylactic or therapeutic vaccines and immunotherapies call for conclusive detection, analysis and sorting of antigen-specific T cells. This is possible by means of soluble recombinant ligands for T cells i.e. MHC class I-peptide (pMHC I) complexes for CD8+ T cells and MHC class II-peptide (pMHC II) complexes for CD4+ T cells and flow cytometry. Here we review major developments in the development of pMHC staining reagents and their diverse applications and discuss perspectives of their use for basic and clinical investigations.http://journal.frontiersin.org/Journal/10.3389/fimmu.2013.00218/fullFlow CytometryVaccinationT cellsT cell receptorcoreceptorMHC I
collection DOAJ
language English
format Article
sources DOAJ
author Julien eSchmidt
Danijel eDojcinovic
Philippe eGuillaume
Immanuel eLuescher
spellingShingle Julien eSchmidt
Danijel eDojcinovic
Philippe eGuillaume
Immanuel eLuescher
ANALYSIS, ISOLATION AND ACTIVATION OF ANTIGEN-SPECIFIC CD4+ AND CD8+ T CELLS BY SOLUBLE MHC-PEPTIDE COMPLEXES
Frontiers in Immunology
Flow Cytometry
Vaccination
T cells
T cell receptor
coreceptor
MHC I
author_facet Julien eSchmidt
Danijel eDojcinovic
Philippe eGuillaume
Immanuel eLuescher
author_sort Julien eSchmidt
title ANALYSIS, ISOLATION AND ACTIVATION OF ANTIGEN-SPECIFIC CD4+ AND CD8+ T CELLS BY SOLUBLE MHC-PEPTIDE COMPLEXES
title_short ANALYSIS, ISOLATION AND ACTIVATION OF ANTIGEN-SPECIFIC CD4+ AND CD8+ T CELLS BY SOLUBLE MHC-PEPTIDE COMPLEXES
title_full ANALYSIS, ISOLATION AND ACTIVATION OF ANTIGEN-SPECIFIC CD4+ AND CD8+ T CELLS BY SOLUBLE MHC-PEPTIDE COMPLEXES
title_fullStr ANALYSIS, ISOLATION AND ACTIVATION OF ANTIGEN-SPECIFIC CD4+ AND CD8+ T CELLS BY SOLUBLE MHC-PEPTIDE COMPLEXES
title_full_unstemmed ANALYSIS, ISOLATION AND ACTIVATION OF ANTIGEN-SPECIFIC CD4+ AND CD8+ T CELLS BY SOLUBLE MHC-PEPTIDE COMPLEXES
title_sort analysis, isolation and activation of antigen-specific cd4+ and cd8+ t cells by soluble mhc-peptide complexes
publisher Frontiers Media S.A.
series Frontiers in Immunology
issn 1664-3224
publishDate 2013-07-01
description T cells constitute the core of adaptive cellular immunity and protect higher organisms against pathogen infections and cancer. Monitoring of disease progression as well as prophylactic or therapeutic vaccines and immunotherapies call for conclusive detection, analysis and sorting of antigen-specific T cells. This is possible by means of soluble recombinant ligands for T cells i.e. MHC class I-peptide (pMHC I) complexes for CD8+ T cells and MHC class II-peptide (pMHC II) complexes for CD4+ T cells and flow cytometry. Here we review major developments in the development of pMHC staining reagents and their diverse applications and discuss perspectives of their use for basic and clinical investigations.
topic Flow Cytometry
Vaccination
T cells
T cell receptor
coreceptor
MHC I
url http://journal.frontiersin.org/Journal/10.3389/fimmu.2013.00218/full
work_keys_str_mv AT julieneschmidt analysisisolationandactivationofantigenspecificcd4andcd8tcellsbysolublemhcpeptidecomplexes
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AT philippeeguillaume analysisisolationandactivationofantigenspecificcd4andcd8tcellsbysolublemhcpeptidecomplexes
AT immanueleluescher analysisisolationandactivationofantigenspecificcd4andcd8tcellsbysolublemhcpeptidecomplexes
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