Effect of aquatic extracts of oleander and Melia azedarach on the nucleic acid content of trichomonas vaginalis in vivo

• Main Authors: Rana Abdullah, Hussain Artain
• Format: Article
• Language: Arabic
• Published: College of Education for Pure Sciences 2007-09-01
• Series: مجلة التربية والعلم
• Subjects: aquatic extracts; oleander; melia azedarach
• Online Access: https://edusj.mosuljournals.com/article_162945_e62ee99aab36515c20835982531c5e2f.pdf

This study was conducted to elucidate the effect of aqueous leaf extract<br />of Nerium oleander and fruit extract of Melia azedarach on nucleic acids<br />content and activity of some enzymes in Trichomonas vagina/is which<br />growth in Diamond's medium tryptone, yeast and maltose (TYM) media.<br />Results indicated that the exposure of T. vagina/is to IC50<br />concentration of N. oleander leaves and M azedarach fruits extracts<br />showed asignificant decrease in DNA content by 31.5% and 38.2%,<br />respectively, while the RNA content decreased by 28.3% and 50.7%,<br />respectively. Furthermore the totel.l. nucleic acids content decreased by<br />27.9% and 48.1% , respectively, compared with the untreated<br />trichomonads with plant extracts after 72 hours of growth.<br />The results of this study also indicated that T. vagina/is treated with<br />N. oleander leaves and M azedarach fruits extracts showed inhibed in the<br />activity of thymidine phosphorylase enzyme at rate 32.7% and 69.2%<br />respectively compared with untreated trichomonads with plant extracts<br />after 72 hours of growth.<br />From the results of this study, 1t is obvious that the above mentioned<br />extracts possess an inhibitory effect on nucleic acids and on the activity<br />of the enzyme Thymidine phosphorylase.