The molecular architecture of engulfment during Bacillus subtilis sporulation
The study of bacterial cell biology is limited by difficulties in visualizing cellular structures at high spatial resolution within their native milieu. Here, we visualize Bacillus subtilis sporulation using cryo-electron tomography coupled with cryo-focused ion beam milling, allowing the reconstruc...
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doaj-56d2c41b0fad498fa79b18682d93915d2021-05-05T17:45:08ZengeLife Sciences Publications LtdeLife2050-084X2019-07-01810.7554/eLife.45257The molecular architecture of engulfment during Bacillus subtilis sporulationKanika Khanna0https://orcid.org/0000-0001-7150-0350Javier Lopez-Garrido1https://orcid.org/0000-0002-8907-502XZiyi Zhao2https://orcid.org/0000-0003-4455-3224Reika Watanabe3https://orcid.org/0000-0002-7427-7702Yuan Yuan4https://orcid.org/0000-0002-9509-9167Joseph Sugie5https://orcid.org/0000-0003-2911-1807Kit Pogliano6https://orcid.org/0000-0002-7868-3345Elizabeth Villa7https://orcid.org/0000-0003-4677-9809Division of Biological Sciences, University of California, San Diego, La Jolla, United StatesDivision of Biological Sciences, University of California, San Diego, La Jolla, United StatesDivision of Biological Sciences, University of California, San Diego, La Jolla, United StatesDivision of Biological Sciences, University of California, San Diego, La Jolla, United StatesDivision of Biological Sciences, University of California, San Diego, La Jolla, United StatesDivision of Biological Sciences, University of California, San Diego, La Jolla, United StatesDivision of Biological Sciences, University of California, San Diego, La Jolla, United StatesDivision of Biological Sciences, University of California, San Diego, La Jolla, United StatesThe study of bacterial cell biology is limited by difficulties in visualizing cellular structures at high spatial resolution within their native milieu. Here, we visualize Bacillus subtilis sporulation using cryo-electron tomography coupled with cryo-focused ion beam milling, allowing the reconstruction of native-state cellular sections at molecular resolution. During sporulation, an asymmetrically-positioned septum generates a larger mother cell and a smaller forespore. Subsequently, the mother cell engulfs the forespore. We show that the septal peptidoglycan is not completely degraded at the onset of engulfment. Instead, the septum is uniformly and only slightly thinned as it curves towards the mother cell. Then, the mother cell membrane migrates around the forespore in tiny finger-like projections, whose formation requires the mother cell SpoIIDMP protein complex. We propose that a limited number of SpoIIDMP complexes tether to and degrade the peptidoglycan ahead of the engulfing membrane, generating an irregular membrane front.https://elifesciences.org/articles/45257engulfmentspoIIDMPmembrane migrationpeptidoglycancryo-focused ion beam millingcryo-electron tomography |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kanika Khanna Javier Lopez-Garrido Ziyi Zhao Reika Watanabe Yuan Yuan Joseph Sugie Kit Pogliano Elizabeth Villa |
spellingShingle |
Kanika Khanna Javier Lopez-Garrido Ziyi Zhao Reika Watanabe Yuan Yuan Joseph Sugie Kit Pogliano Elizabeth Villa The molecular architecture of engulfment during Bacillus subtilis sporulation eLife engulfment spoIIDMP membrane migration peptidoglycan cryo-focused ion beam milling cryo-electron tomography |
author_facet |
Kanika Khanna Javier Lopez-Garrido Ziyi Zhao Reika Watanabe Yuan Yuan Joseph Sugie Kit Pogliano Elizabeth Villa |
author_sort |
Kanika Khanna |
title |
The molecular architecture of engulfment during Bacillus subtilis sporulation |
title_short |
The molecular architecture of engulfment during Bacillus subtilis sporulation |
title_full |
The molecular architecture of engulfment during Bacillus subtilis sporulation |
title_fullStr |
The molecular architecture of engulfment during Bacillus subtilis sporulation |
title_full_unstemmed |
The molecular architecture of engulfment during Bacillus subtilis sporulation |
title_sort |
molecular architecture of engulfment during bacillus subtilis sporulation |
publisher |
eLife Sciences Publications Ltd |
series |
eLife |
issn |
2050-084X |
publishDate |
2019-07-01 |
description |
The study of bacterial cell biology is limited by difficulties in visualizing cellular structures at high spatial resolution within their native milieu. Here, we visualize Bacillus subtilis sporulation using cryo-electron tomography coupled with cryo-focused ion beam milling, allowing the reconstruction of native-state cellular sections at molecular resolution. During sporulation, an asymmetrically-positioned septum generates a larger mother cell and a smaller forespore. Subsequently, the mother cell engulfs the forespore. We show that the septal peptidoglycan is not completely degraded at the onset of engulfment. Instead, the septum is uniformly and only slightly thinned as it curves towards the mother cell. Then, the mother cell membrane migrates around the forespore in tiny finger-like projections, whose formation requires the mother cell SpoIIDMP protein complex. We propose that a limited number of SpoIIDMP complexes tether to and degrade the peptidoglycan ahead of the engulfing membrane, generating an irregular membrane front. |
topic |
engulfment spoIIDMP membrane migration peptidoglycan cryo-focused ion beam milling cryo-electron tomography |
url |
https://elifesciences.org/articles/45257 |
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