Co-expression of CMTM6 and PD-L1: a novel prognostic indicator of gastric cancer
Abstract Background CKLF Like MARVEL Transmembrane Domain Containing 6 (CMTM6) is involved in the epigenetic regulation of genes and tumorigenesis. Programmed cell death ligand 1 (PD-L1) is closely related to the prognosis of some human cancers. CMTM6 is a key regulator of PD-L1 in many cancers. The...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2021-01-01
|
Series: | Cancer Cell International |
Subjects: | |
Online Access: | https://doi.org/10.1186/s12935-020-01734-6 |
id |
doaj-56d7e574c880452fb8f77871d53eaf8e |
---|---|
record_format |
Article |
spelling |
doaj-56d7e574c880452fb8f77871d53eaf8e2021-01-31T16:09:58ZengBMCCancer Cell International1475-28672021-01-012111910.1186/s12935-020-01734-6Co-expression of CMTM6 and PD-L1: a novel prognostic indicator of gastric cancerChao Zhang0Shutao Zhao1Xudong Wang2Department of Gastrointestinal Nutrition and Hernia Surgery, The Second Hospital of Jilin UniversityDepartment of Gastrointestinal Nutrition and Hernia Surgery, The Second Hospital of Jilin UniversityDepartment of Gastrointestinal Nutrition and Hernia Surgery, The Second Hospital of Jilin UniversityAbstract Background CKLF Like MARVEL Transmembrane Domain Containing 6 (CMTM6) is involved in the epigenetic regulation of genes and tumorigenesis. Programmed cell death ligand 1 (PD-L1) is closely related to the prognosis of some human cancers. CMTM6 is a key regulator of PD-L1 in many cancers. The purpose of this study was to investigate the expressions of these proteins in gastric cancer and the correlations with clinicopathological features and survival. Methods The expression levels of CMTM6 and PD-L1 were examined in 185 gastric cancer specimens using immunohistochemistry, quantitative real-time PCR and Western blot. Immunofluorescence was used to examine the localizations of CMTM6 and PD-L1. Chi-square test was used to analyze the relationship between CMTM6 and PD-L1 expressions and clinicopathological characteristics. Kaplan–Meier method and log-rank test were used to analyze the survival data of patients. Results The positive expression rates of CMTM6 and PD-L1 in gastric cancers were 78.38% (145/185) and 75.68% (140/185), respectively. CMTM6 and PD-L1 were both mainly expressed in the cell membrane and nucleus of gastric cancer tumor cells. High expression of CMTM6 and PD-L1 was correlated with Borrmann type (P < 0.001), N stage (P = 0.002), peritoneal metastasis (P = 0.007) and TNM stage (P = 0.038). CMTM6 and PD-L1 expression in gastric cancer tissues showed a positive correlation (Pearson’s coefficient test, r = 0.260; P < 0.001). CMTM6 may positively regulate PD-L1 expression. High expression of CMTM6 was correlated with poor prognosis of gastric cancer patients (HR = 1.668; 95% CI = 1.032–2.695; P = 0.037). High expression of both CMTM6 and PD-L1 may be an independent factor for overall survival (HR = 1.554; 95% CI = 1.011–2.389; P = 0.044). Conclusion The combined detection of CMTM6 and PD-L1 may be used as an indicator for judging the prognosis of gastric cancer patients.https://doi.org/10.1186/s12935-020-01734-6Gastric cancerCMTM6PD-L1Survival |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chao Zhang Shutao Zhao Xudong Wang |
spellingShingle |
Chao Zhang Shutao Zhao Xudong Wang Co-expression of CMTM6 and PD-L1: a novel prognostic indicator of gastric cancer Cancer Cell International Gastric cancer CMTM6 PD-L1 Survival |
author_facet |
Chao Zhang Shutao Zhao Xudong Wang |
author_sort |
Chao Zhang |
title |
Co-expression of CMTM6 and PD-L1: a novel prognostic indicator of gastric cancer |
title_short |
Co-expression of CMTM6 and PD-L1: a novel prognostic indicator of gastric cancer |
title_full |
Co-expression of CMTM6 and PD-L1: a novel prognostic indicator of gastric cancer |
title_fullStr |
Co-expression of CMTM6 and PD-L1: a novel prognostic indicator of gastric cancer |
title_full_unstemmed |
Co-expression of CMTM6 and PD-L1: a novel prognostic indicator of gastric cancer |
title_sort |
co-expression of cmtm6 and pd-l1: a novel prognostic indicator of gastric cancer |
publisher |
BMC |
series |
Cancer Cell International |
issn |
1475-2867 |
publishDate |
2021-01-01 |
description |
Abstract Background CKLF Like MARVEL Transmembrane Domain Containing 6 (CMTM6) is involved in the epigenetic regulation of genes and tumorigenesis. Programmed cell death ligand 1 (PD-L1) is closely related to the prognosis of some human cancers. CMTM6 is a key regulator of PD-L1 in many cancers. The purpose of this study was to investigate the expressions of these proteins in gastric cancer and the correlations with clinicopathological features and survival. Methods The expression levels of CMTM6 and PD-L1 were examined in 185 gastric cancer specimens using immunohistochemistry, quantitative real-time PCR and Western blot. Immunofluorescence was used to examine the localizations of CMTM6 and PD-L1. Chi-square test was used to analyze the relationship between CMTM6 and PD-L1 expressions and clinicopathological characteristics. Kaplan–Meier method and log-rank test were used to analyze the survival data of patients. Results The positive expression rates of CMTM6 and PD-L1 in gastric cancers were 78.38% (145/185) and 75.68% (140/185), respectively. CMTM6 and PD-L1 were both mainly expressed in the cell membrane and nucleus of gastric cancer tumor cells. High expression of CMTM6 and PD-L1 was correlated with Borrmann type (P < 0.001), N stage (P = 0.002), peritoneal metastasis (P = 0.007) and TNM stage (P = 0.038). CMTM6 and PD-L1 expression in gastric cancer tissues showed a positive correlation (Pearson’s coefficient test, r = 0.260; P < 0.001). CMTM6 may positively regulate PD-L1 expression. High expression of CMTM6 was correlated with poor prognosis of gastric cancer patients (HR = 1.668; 95% CI = 1.032–2.695; P = 0.037). High expression of both CMTM6 and PD-L1 may be an independent factor for overall survival (HR = 1.554; 95% CI = 1.011–2.389; P = 0.044). Conclusion The combined detection of CMTM6 and PD-L1 may be used as an indicator for judging the prognosis of gastric cancer patients. |
topic |
Gastric cancer CMTM6 PD-L1 Survival |
url |
https://doi.org/10.1186/s12935-020-01734-6 |
work_keys_str_mv |
AT chaozhang coexpressionofcmtm6andpdl1anovelprognosticindicatorofgastriccancer AT shutaozhao coexpressionofcmtm6andpdl1anovelprognosticindicatorofgastriccancer AT xudongwang coexpressionofcmtm6andpdl1anovelprognosticindicatorofgastriccancer |
_version_ |
1724316729271648256 |