Experience With the Use of the MicroDTTect Device for the Diagnosis of Low-Grade Chronic Prosthetic Joint Infections in a Routine Setting
Background: In prosthetic joint infections (PJIs), identification of the causative microorganisms is critical to successfully adapt and optimize treatment. However, microbiological diagnosis of PJIs remains a challenge notably because bacteria are embedded in biofilm adhered to the prosthetic materi...
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Frontiers Media S.A.
2021-03-01
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Online Access: | https://www.frontiersin.org/articles/10.3389/fmed.2021.565555/full |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Camille Kolenda Camille Kolenda Camille Kolenda Jérôme Josse Jérôme Josse Cécile Batailler Cécile Batailler Allison Faure Alice Monteix Sébastien Lustig Sébastien Lustig Tristan Ferry Tristan Ferry Tristan Ferry Frédéric Laurent Frédéric Laurent Frédéric Laurent Céline Dupieux Céline Dupieux Céline Dupieux |
spellingShingle |
Camille Kolenda Camille Kolenda Camille Kolenda Jérôme Josse Jérôme Josse Cécile Batailler Cécile Batailler Allison Faure Alice Monteix Sébastien Lustig Sébastien Lustig Tristan Ferry Tristan Ferry Tristan Ferry Frédéric Laurent Frédéric Laurent Frédéric Laurent Céline Dupieux Céline Dupieux Céline Dupieux Experience With the Use of the MicroDTTect Device for the Diagnosis of Low-Grade Chronic Prosthetic Joint Infections in a Routine Setting Frontiers in Medicine dithiothreitol prosthetic joint infection bone and joint infection microbiological diagnosis biofilm implant failure |
author_facet |
Camille Kolenda Camille Kolenda Camille Kolenda Jérôme Josse Jérôme Josse Cécile Batailler Cécile Batailler Allison Faure Alice Monteix Sébastien Lustig Sébastien Lustig Tristan Ferry Tristan Ferry Tristan Ferry Frédéric Laurent Frédéric Laurent Frédéric Laurent Céline Dupieux Céline Dupieux Céline Dupieux |
author_sort |
Camille Kolenda |
title |
Experience With the Use of the MicroDTTect Device for the Diagnosis of Low-Grade Chronic Prosthetic Joint Infections in a Routine Setting |
title_short |
Experience With the Use of the MicroDTTect Device for the Diagnosis of Low-Grade Chronic Prosthetic Joint Infections in a Routine Setting |
title_full |
Experience With the Use of the MicroDTTect Device for the Diagnosis of Low-Grade Chronic Prosthetic Joint Infections in a Routine Setting |
title_fullStr |
Experience With the Use of the MicroDTTect Device for the Diagnosis of Low-Grade Chronic Prosthetic Joint Infections in a Routine Setting |
title_full_unstemmed |
Experience With the Use of the MicroDTTect Device for the Diagnosis of Low-Grade Chronic Prosthetic Joint Infections in a Routine Setting |
title_sort |
experience with the use of the microdttect device for the diagnosis of low-grade chronic prosthetic joint infections in a routine setting |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Medicine |
issn |
2296-858X |
publishDate |
2021-03-01 |
description |
Background: In prosthetic joint infections (PJIs), identification of the causative microorganisms is critical to successfully adapt and optimize treatment. However, microbiological diagnosis of PJIs remains a challenge notably because bacteria are embedded in biofilm adhered to the prosthetic material. Recently, dithiothreitol (DTT) treatment of prosthesis has been proposed as a new strategy to release bacteria from biofilm and to improve the yield of microbiological diagnosis. In this study, we evaluated the interest of a commercial device using DTT, the MicroDTTect system (Heraeus, Hanau, Germany), for the diagnosis of low-grade chronic PJIs, compared to the conventional culture of periprosthetic tissue (PPT) samples.Methods: Twenty patients undergoing a surgery procedure for removal of prosthetic material because of a suspicion of low-grade PJI without pre-operative microbiological documentation were included (NCT04371068). Bacteriological results using the fluid obtained after prosthesis treatment with the MicroDTTect system were compared to results obtained with conventional culture of PPT samples.Results: All the bacteria considered as responsible for PJIs recovered from culture of PPT samples were also detected using the MicroDTTect device. For one patient, an additional bacterial isolate (Staphylococcus haemolyticus) suspected to be involved in a polymicrobial PJI was identified using DTT treatment. Time to positivity of the cultures was also reduced using the MicroDTTect system, notably in case of Cutibacterium acnes infection. However, probable bacterial contaminants were found (MicroDTTect system, n = 5; PPT samples, n = 1).Conclusion: This study showed that DTT treatment of the prosthetic component using the MicroDTTect device could improve the microbiological diagnosis of low-grade PJIs. |
topic |
dithiothreitol prosthetic joint infection bone and joint infection microbiological diagnosis biofilm implant failure |
url |
https://www.frontiersin.org/articles/10.3389/fmed.2021.565555/full |
work_keys_str_mv |
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doaj-56f8dddb8412451b9758a93b2c6eb42a2021-03-16T04:35:01ZengFrontiers Media S.A.Frontiers in Medicine2296-858X2021-03-01810.3389/fmed.2021.565555565555Experience With the Use of the MicroDTTect Device for the Diagnosis of Low-Grade Chronic Prosthetic Joint Infections in a Routine SettingCamille Kolenda0Camille Kolenda1Camille Kolenda2Jérôme Josse3Jérôme Josse4Cécile Batailler5Cécile Batailler6Allison Faure7Alice Monteix8Sébastien Lustig9Sébastien Lustig10Tristan Ferry11Tristan Ferry12Tristan Ferry13Frédéric Laurent14Frédéric Laurent15Frédéric Laurent16Céline Dupieux17Céline Dupieux18Céline Dupieux19CIRI, Centre International de Recherche en Infectiologie, Inserm U1111, Université Claude Bernard Lyon 1, CNRS UMR5308, ENS de Lyon, Lyon, FranceCentre de Référence des Infections Ostéo-Articulaires Complexes de Lyon, Lyon, FranceLaboratoire de Bactériologie, Institut des Agents Infectieux, Hôpital de la Croix-Rousse, Hospices Civils de Lyon, Lyon, FranceCIRI, Centre International de Recherche en Infectiologie, Inserm U1111, Université Claude Bernard Lyon 1, CNRS UMR5308, ENS de Lyon, Lyon, FranceCentre de Référence des Infections Ostéo-Articulaires Complexes de Lyon, Lyon, FranceCentre de Référence des Infections Ostéo-Articulaires Complexes de Lyon, Lyon, FranceService de Chirurgie Orthopédique, Hôpital de la Croix-Rousse, Hospices Civils de Lyon, Lyon, FranceCIRI, Centre International de Recherche en Infectiologie, Inserm U1111, Université Claude Bernard Lyon 1, CNRS UMR5308, ENS de Lyon, Lyon, FranceLaboratoire de Bactériologie, Institut des Agents Infectieux, Hôpital de la Croix-Rousse, Hospices Civils de Lyon, Lyon, FranceCentre de Référence des Infections Ostéo-Articulaires Complexes de Lyon, Lyon, FranceService de Chirurgie Orthopédique, Hôpital de la Croix-Rousse, Hospices Civils de Lyon, Lyon, FranceCIRI, Centre International de Recherche en Infectiologie, Inserm U1111, Université Claude Bernard Lyon 1, CNRS UMR5308, ENS de Lyon, Lyon, FranceCentre de Référence des Infections Ostéo-Articulaires Complexes de Lyon, Lyon, FranceService de Maladies Infectieuses et Tropicales, Hôpital de la Croix-Rousse, Hospices Civils de Lyon, Lyon, FranceCIRI, Centre International de Recherche en Infectiologie, Inserm U1111, Université Claude Bernard Lyon 1, CNRS UMR5308, ENS de Lyon, Lyon, FranceCentre de Référence des Infections Ostéo-Articulaires Complexes de Lyon, Lyon, FranceLaboratoire de Bactériologie, Institut des Agents Infectieux, Hôpital de la Croix-Rousse, Hospices Civils de Lyon, Lyon, FranceCIRI, Centre International de Recherche en Infectiologie, Inserm U1111, Université Claude Bernard Lyon 1, CNRS UMR5308, ENS de Lyon, Lyon, FranceCentre de Référence des Infections Ostéo-Articulaires Complexes de Lyon, Lyon, FranceLaboratoire de Bactériologie, Institut des Agents Infectieux, Hôpital de la Croix-Rousse, Hospices Civils de Lyon, Lyon, FranceBackground: In prosthetic joint infections (PJIs), identification of the causative microorganisms is critical to successfully adapt and optimize treatment. However, microbiological diagnosis of PJIs remains a challenge notably because bacteria are embedded in biofilm adhered to the prosthetic material. Recently, dithiothreitol (DTT) treatment of prosthesis has been proposed as a new strategy to release bacteria from biofilm and to improve the yield of microbiological diagnosis. In this study, we evaluated the interest of a commercial device using DTT, the MicroDTTect system (Heraeus, Hanau, Germany), for the diagnosis of low-grade chronic PJIs, compared to the conventional culture of periprosthetic tissue (PPT) samples.Methods: Twenty patients undergoing a surgery procedure for removal of prosthetic material because of a suspicion of low-grade PJI without pre-operative microbiological documentation were included (NCT04371068). Bacteriological results using the fluid obtained after prosthesis treatment with the MicroDTTect system were compared to results obtained with conventional culture of PPT samples.Results: All the bacteria considered as responsible for PJIs recovered from culture of PPT samples were also detected using the MicroDTTect device. For one patient, an additional bacterial isolate (Staphylococcus haemolyticus) suspected to be involved in a polymicrobial PJI was identified using DTT treatment. Time to positivity of the cultures was also reduced using the MicroDTTect system, notably in case of Cutibacterium acnes infection. However, probable bacterial contaminants were found (MicroDTTect system, n = 5; PPT samples, n = 1).Conclusion: This study showed that DTT treatment of the prosthetic component using the MicroDTTect device could improve the microbiological diagnosis of low-grade PJIs.https://www.frontiersin.org/articles/10.3389/fmed.2021.565555/fulldithiothreitolprosthetic joint infectionbone and joint infectionmicrobiological diagnosisbiofilmimplant failure |