Immunohistological characterization of myoepithelial cells using monoclonal antibodies
Aim: The aim of this study was to investigate the expression of monoclonal antibody (mAbs) in the myoepithelial cells of the rat submandibular salivary glands (SSG) during postnatal development. Material and method: SSG were obtained from 1, 30 and 60 days old, male, AO rats. Streptavidin-biotin imm...
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Serbian Medical Society - Dental Section, Belgrade
2008-01-01
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Online Access: | http://www.doiserbia.nb.rs/img/doi/0039-1743/2008/0039-17430801007D.pdf |
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doaj-57069b24ee354bd7a9e38527925309512020-11-24T21:02:20ZengSerbian Medical Society - Dental Section, BelgradeStomatološki glasnik Srbije0039-17432008-01-0155171410.2298/SGS0801007DImmunohistological characterization of myoepithelial cells using monoclonal antibodiesDožić IvanČolić MiodragAim: The aim of this study was to investigate the expression of monoclonal antibody (mAbs) in the myoepithelial cells of the rat submandibular salivary glands (SSG) during postnatal development. Material and method: SSG were obtained from 1, 30 and 60 days old, male, AO rats. Streptavidin-biotin immunoperoxidase method was used for immunohistochemistry. Cryostat sections were fixed in acetone and incubated with mAbs which specifically reacts with cytokeratins (CK) polypeptides ( CK7, CK8, CK18, CK kidney, K8.12, K8.13), rats epithelial (PT13D11), rats collagen (RMC-23), laminin (α6β1) and rats sialoadhesin (ED3). Results: Immunohistochemical analysis of the myoepithelial cells showed phenotypic heterogeneity during the postnatal development. Myoepithelial cells of acini were stained by K8.13, PT13D11 and ED3 mAbs, whereas these cells were not stained by mAbs CK7, CK8, CK18, CK-kidney, RMC-23 and α6β1. In adult rats, myoepithelial cells of excretory duct were stained by K8.12 mAb. Conclusion: The obtained results show phenotypic heterogeneity of myoepithelial cells and might also be useful to further understanding of myoepithelial cells development and functions. http://www.doiserbia.nb.rs/img/doi/0039-1743/2008/0039-17430801007D.pdfmyoepithelial cellssubmandibular salivary glandsmonoclonal antibodyontogenesis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Dožić Ivan Čolić Miodrag |
spellingShingle |
Dožić Ivan Čolić Miodrag Immunohistological characterization of myoepithelial cells using monoclonal antibodies Stomatološki glasnik Srbije myoepithelial cells submandibular salivary glands monoclonal antibody ontogenesis |
author_facet |
Dožić Ivan Čolić Miodrag |
author_sort |
Dožić Ivan |
title |
Immunohistological characterization of myoepithelial cells using monoclonal antibodies |
title_short |
Immunohistological characterization of myoepithelial cells using monoclonal antibodies |
title_full |
Immunohistological characterization of myoepithelial cells using monoclonal antibodies |
title_fullStr |
Immunohistological characterization of myoepithelial cells using monoclonal antibodies |
title_full_unstemmed |
Immunohistological characterization of myoepithelial cells using monoclonal antibodies |
title_sort |
immunohistological characterization of myoepithelial cells using monoclonal antibodies |
publisher |
Serbian Medical Society - Dental Section, Belgrade |
series |
Stomatološki glasnik Srbije |
issn |
0039-1743 |
publishDate |
2008-01-01 |
description |
Aim: The aim of this study was to investigate the expression of monoclonal antibody (mAbs) in the myoepithelial cells of the rat submandibular salivary glands (SSG) during postnatal development. Material and method: SSG were obtained from 1, 30 and 60 days old, male, AO rats. Streptavidin-biotin immunoperoxidase method was used for immunohistochemistry. Cryostat sections were fixed in acetone and incubated with mAbs which specifically reacts with cytokeratins (CK) polypeptides ( CK7, CK8, CK18, CK kidney, K8.12, K8.13), rats epithelial (PT13D11), rats collagen (RMC-23), laminin (α6β1) and rats sialoadhesin (ED3). Results: Immunohistochemical analysis of the myoepithelial cells showed phenotypic heterogeneity during the postnatal development. Myoepithelial cells of acini were stained by K8.13, PT13D11 and ED3 mAbs, whereas these cells were not stained by mAbs CK7, CK8, CK18, CK-kidney, RMC-23 and α6β1. In adult rats, myoepithelial cells of excretory duct were stained by K8.12 mAb. Conclusion: The obtained results show phenotypic heterogeneity of myoepithelial cells and might also be useful to further understanding of myoepithelial cells development and functions. |
topic |
myoepithelial cells submandibular salivary glands monoclonal antibody ontogenesis |
url |
http://www.doiserbia.nb.rs/img/doi/0039-1743/2008/0039-17430801007D.pdf |
work_keys_str_mv |
AT dozicivan immunohistologicalcharacterizationofmyoepithelialcellsusingmonoclonalantibodies AT colicmiodrag immunohistologicalcharacterizationofmyoepithelialcellsusingmonoclonalantibodies |
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1716775675221770240 |