Differential Activation of TRP Channels in the Adult Rat Spinal Substantia Gelatinosa by Stereoisomers of Plant-Derived Chemicals
Activation of TRPV1, TRPA1 or TRPM8 channel expressed in the central terminal of dorsal root ganglion (DRG) neuron increases the spontaneous release of l-glutamate onto spinal dorsal horn lamina II (substantia gelatinosa; SG) neurons which play a pivotal role in regulating nociceptive transmission....
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doaj-570a94fd69a04aebb8b750312de7b2232020-11-25T02:58:16ZengMDPI AGPharmaceuticals1424-82472016-07-01934610.3390/ph9030046ph9030046Differential Activation of TRP Channels in the Adult Rat Spinal Substantia Gelatinosa by Stereoisomers of Plant-Derived ChemicalsEiichi Kumamoto0Tsugumi Fujita1Department of Physiology, Saga Medical School, 5-1-1 Nabeshima, Saga 849-8501, JapanDepartment of Physiology, Saga Medical School, 5-1-1 Nabeshima, Saga 849-8501, JapanActivation of TRPV1, TRPA1 or TRPM8 channel expressed in the central terminal of dorsal root ganglion (DRG) neuron increases the spontaneous release of l-glutamate onto spinal dorsal horn lamina II (substantia gelatinosa; SG) neurons which play a pivotal role in regulating nociceptive transmission. The TRP channels are activated by various plant-derived chemicals. Although stereoisomers activate or modulate ion channels in a distinct manner, this phenomenon is not fully addressed for TRP channels. By applying the whole-cell patch-clamp technique to SG neurons of adult rat spinal cord slices, we found out that all of plant-derived chemicals, carvacrol, thymol, carvone and cineole, increase the frequency of spontaneous excitatory postsynaptic current, a measure of the spontaneous release of l-glutamate from nerve terminals, by activating TRP channels. The presynaptic activities were different between stereoisomers (carvacrol and thymol; (−)-carvone and (+)-carvone; 1,8-cineole and 1,4-cineole) in the extent or the types of TRP channels activated, indicating that TRP channels in the SG are activated by stereoisomers in a distinct manner. This result could serve to know the properties of the central terminal TRP channels that are targets of drugs for alleviating pain.http://www.mdpi.com/1424-8247/9/3/46TRPV1TRPA1TRPM8plant-derived chemical">l-glutamate releasespinal dorsal hornpatch-clamprat |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Eiichi Kumamoto Tsugumi Fujita |
spellingShingle |
Eiichi Kumamoto Tsugumi Fujita Differential Activation of TRP Channels in the Adult Rat Spinal Substantia Gelatinosa by Stereoisomers of Plant-Derived Chemicals Pharmaceuticals TRPV1 TRPA1 TRPM8 plant-derived chemical ">l-glutamate release spinal dorsal horn patch-clamp rat |
author_facet |
Eiichi Kumamoto Tsugumi Fujita |
author_sort |
Eiichi Kumamoto |
title |
Differential Activation of TRP Channels in the Adult Rat Spinal Substantia Gelatinosa by Stereoisomers of Plant-Derived Chemicals |
title_short |
Differential Activation of TRP Channels in the Adult Rat Spinal Substantia Gelatinosa by Stereoisomers of Plant-Derived Chemicals |
title_full |
Differential Activation of TRP Channels in the Adult Rat Spinal Substantia Gelatinosa by Stereoisomers of Plant-Derived Chemicals |
title_fullStr |
Differential Activation of TRP Channels in the Adult Rat Spinal Substantia Gelatinosa by Stereoisomers of Plant-Derived Chemicals |
title_full_unstemmed |
Differential Activation of TRP Channels in the Adult Rat Spinal Substantia Gelatinosa by Stereoisomers of Plant-Derived Chemicals |
title_sort |
differential activation of trp channels in the adult rat spinal substantia gelatinosa by stereoisomers of plant-derived chemicals |
publisher |
MDPI AG |
series |
Pharmaceuticals |
issn |
1424-8247 |
publishDate |
2016-07-01 |
description |
Activation of TRPV1, TRPA1 or TRPM8 channel expressed in the central terminal of dorsal root ganglion (DRG) neuron increases the spontaneous release of l-glutamate onto spinal dorsal horn lamina II (substantia gelatinosa; SG) neurons which play a pivotal role in regulating nociceptive transmission. The TRP channels are activated by various plant-derived chemicals. Although stereoisomers activate or modulate ion channels in a distinct manner, this phenomenon is not fully addressed for TRP channels. By applying the whole-cell patch-clamp technique to SG neurons of adult rat spinal cord slices, we found out that all of plant-derived chemicals, carvacrol, thymol, carvone and cineole, increase the frequency of spontaneous excitatory postsynaptic current, a measure of the spontaneous release of l-glutamate from nerve terminals, by activating TRP channels. The presynaptic activities were different between stereoisomers (carvacrol and thymol; (−)-carvone and (+)-carvone; 1,8-cineole and 1,4-cineole) in the extent or the types of TRP channels activated, indicating that TRP channels in the SG are activated by stereoisomers in a distinct manner. This result could serve to know the properties of the central terminal TRP channels that are targets of drugs for alleviating pain. |
topic |
TRPV1 TRPA1 TRPM8 plant-derived chemical ">l-glutamate release spinal dorsal horn patch-clamp rat |
url |
http://www.mdpi.com/1424-8247/9/3/46 |
work_keys_str_mv |
AT eiichikumamoto differentialactivationoftrpchannelsintheadultratspinalsubstantiagelatinosabystereoisomersofplantderivedchemicals AT tsugumifujita differentialactivationoftrpchannelsintheadultratspinalsubstantiagelatinosabystereoisomersofplantderivedchemicals |
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