Vimentin Intermediate Filament Rings Deform the Nucleus During the First Steps of Adhesion
During cell spreading, cells undergo many changes to their architecture and their mechanical properties. Vimentin, as an integral part of the cell architecture, and its mechanical stability must adapt to the new state of the cell. This study focuses on the structures formed by vimentin during the fi...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2019-06-01
|
Series: | Frontiers in Cell and Developmental Biology |
Subjects: | |
Online Access: | https://www.frontiersin.org/article/10.3389/fcell.2019.00106/full |
id |
doaj-5723c41f58bf45ccacd797db91779c13 |
---|---|
record_format |
Article |
spelling |
doaj-5723c41f58bf45ccacd797db91779c132020-11-25T01:16:30ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2019-06-01710.3389/fcell.2019.00106459443Vimentin Intermediate Filament Rings Deform the Nucleus During the First Steps of AdhesionEmmanuel Terriac0Susanne Schütz1Franziska Lautenschläger2Franziska Lautenschläger3Leibniz Institute for New Materials, Saarbrücken, GermanyFaculty of Natural Sciences and Technology, Saarland University, Saarbrücken, GermanyLeibniz Institute for New Materials, Saarbrücken, GermanyFaculty of Natural Sciences and Technology, Saarland University, Saarbrücken, GermanyDuring cell spreading, cells undergo many changes to their architecture and their mechanical properties. Vimentin, as an integral part of the cell architecture, and its mechanical stability must adapt to the new state of the cell. This study focuses on the structures formed by vimentin during the first steps of cell adhesion. Very early, ball-like structures, or “knots,” are seen and often vimentin filaments emerge in the shape of rings around the nucleus. Although intermediate filaments are not known to be associated to motor proteins to form contractile systems, these rings can nonetheless strongly deform the cell nucleus. In the first 6 to 12 h of adhesion, these vimentin knots and rings disappear, and the intermediate filament network returns to the state seen before detachment of the cells. As these vimentin structures are very transient in the early steps of cell spreading, they have rarely been described in the literature. However, they can also be seen during mitosis, which is an event that involves partial detachment and re-spreading of the cells. Interestingly, the turnover dynamics of vimentin are reduced in both the knots and rings, compared to vimentin in the lamellipodia. It remains to define how the force is transmitted from the ball-like structures to the rings, and to measure the impact of such strong nuclear deformation on gene expression during cell re-spreading and the rearrangement of the vimentin network.https://www.frontiersin.org/article/10.3389/fcell.2019.00106/fullvimentinadhesionnuclear deformationringcell spreading |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Emmanuel Terriac Susanne Schütz Franziska Lautenschläger Franziska Lautenschläger |
spellingShingle |
Emmanuel Terriac Susanne Schütz Franziska Lautenschläger Franziska Lautenschläger Vimentin Intermediate Filament Rings Deform the Nucleus During the First Steps of Adhesion Frontiers in Cell and Developmental Biology vimentin adhesion nuclear deformation ring cell spreading |
author_facet |
Emmanuel Terriac Susanne Schütz Franziska Lautenschläger Franziska Lautenschläger |
author_sort |
Emmanuel Terriac |
title |
Vimentin Intermediate Filament Rings Deform the Nucleus During the First Steps of Adhesion |
title_short |
Vimentin Intermediate Filament Rings Deform the Nucleus During the First Steps of Adhesion |
title_full |
Vimentin Intermediate Filament Rings Deform the Nucleus During the First Steps of Adhesion |
title_fullStr |
Vimentin Intermediate Filament Rings Deform the Nucleus During the First Steps of Adhesion |
title_full_unstemmed |
Vimentin Intermediate Filament Rings Deform the Nucleus During the First Steps of Adhesion |
title_sort |
vimentin intermediate filament rings deform the nucleus during the first steps of adhesion |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Cell and Developmental Biology |
issn |
2296-634X |
publishDate |
2019-06-01 |
description |
During cell spreading, cells undergo many changes to their architecture and their mechanical properties. Vimentin, as an integral part of the cell architecture, and its mechanical stability must adapt to the new state of the cell. This study focuses on the structures formed by vimentin during the first steps of cell adhesion. Very early, ball-like structures, or “knots,” are seen and often vimentin filaments emerge in the shape of rings around the nucleus. Although intermediate filaments are not known to be associated to motor proteins to form contractile systems, these rings can nonetheless strongly deform the cell nucleus. In the first 6 to 12 h of adhesion, these vimentin knots and rings disappear, and the intermediate filament network returns to the state seen before detachment of the cells. As these vimentin structures are very transient in the early steps of cell spreading, they have rarely been described in the literature. However, they can also be seen during mitosis, which is an event that involves partial detachment and re-spreading of the cells. Interestingly, the turnover dynamics of vimentin are reduced in both the knots and rings, compared to vimentin in the lamellipodia. It remains to define how the force is transmitted from the ball-like structures to the rings, and to measure the impact of such strong nuclear deformation on gene expression during cell re-spreading and the rearrangement of the vimentin network. |
topic |
vimentin adhesion nuclear deformation ring cell spreading |
url |
https://www.frontiersin.org/article/10.3389/fcell.2019.00106/full |
work_keys_str_mv |
AT emmanuelterriac vimentinintermediatefilamentringsdeformthenucleusduringthefirststepsofadhesion AT susanneschutz vimentinintermediatefilamentringsdeformthenucleusduringthefirststepsofadhesion AT franziskalautenschlager vimentinintermediatefilamentringsdeformthenucleusduringthefirststepsofadhesion AT franziskalautenschlager vimentinintermediatefilamentringsdeformthenucleusduringthefirststepsofadhesion |
_version_ |
1725149781576122368 |