Effect of combination of Melatonin and All-Trans retinoic acid on maturation, fertilization and embryonic development of immature mouse oocytes
Background and Objective: With respect to the antioxidant role of melatonin and retinoic acid, it seems to be effective both in the maturation and embryonic development. This study was done to investigate the effect of combination of melatonin and All-Trans retinoic acid (RA) on maturation, fertiliz...
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Golestan University of Medical Sciences
2015-09-01
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doaj-572a13afdf9e47b2ab6c60941b8a6f462020-11-24T20:41:46ZfasGolestan University of Medical Sciences مجله دانشگاه علوم پزشکی گرگان1562-47652008-40802015-09-011734654Effect of combination of Melatonin and All-Trans retinoic acid on maturation, fertilization and embryonic development of immature mouse oocytesTadayoni S0Malekzadeh Shafarodi M1Ghasemi Hamidabadi H2Esmailnejad Moghaddam A3Khalilian A4Rezaei N5 M.Sc Student of Anatomical Sciences, Faculty of Medicine, Mazandaran Univercity of Medical Sciences, Sari, Iran Assistant Professor, Department of Anatomical Sciences, Faculty of Medicine, Mazandaran Univercity of Medical Sciences, Sari, Iran Assistant Professor, Department of Anatomical Sciences, Faculty of Medicine, Mazandaran Univercity of Medical Sciences, Sari, Iran Associate Professor, Department of Anatomical Sciences, Faculty of Medicine, Mazandaran Univercity of Medical Sciences, Sari, Iran Professor of Biostatistics, Department of Community Medicine, Faculty of Medicine, Research Center for Molecular Medicine, Mazandaran Univercity of Medical Sciences, Sari, Iran Associate Professor, Department of Anatomical Sciences, Faculty of Medicine, Research Center for Molecular Medicine, Mazandaran Univercity of Medical Sciences, Sari, Iran Background and Objective: With respect to the antioxidant role of melatonin and retinoic acid, it seems to be effective both in the maturation and embryonic development. This study was done to investigate the effect of combination of melatonin and All-Trans retinoic acid (RA) on maturation, fertilization and embryonic development of immature mouse oocytes. Methods: In this experimental study, cumulus - oocyte complex (COCs) were recovered from 4-6 week old female mice NMRI and were divided into 6 maturation medium groups including control, sham, experiment 1(melatonin 100 nM, 1 and 2 µM), experiment 2 (retinoic acid 1, 2, 4, 6 µM), experiment 3 (melatonin 2 µM+RA 4 µM), experiment 4 (Mel 100nM + retinoic acid 4µM). The maturation rate was recorded after 24 hours of culture in a humidified atmosphere of 5% CO2 at 37°C. The matured oocytes were fertilized with sperm. Fertilization and embryonic development rates to the blastocyst stage were recorded. Results: Maturation rate in the control and sham groups were 50.6% and 49.4%, respectively. Maturation rate were 54.3%, 54.8%, 59.9% in melatonin group with concentrations of 100 nM, 1 and 2 µM, respectively. Maturation rate were 51.6%, 51%, 59% and 49.6% in t-RA group with concentrations of 1, 2, 4, 6 μM. Maturation rate were 60.4% and 54.2% in the experiment 3 and 4 groups, respectively. The maturation rates in the melatonin 2 µM, retinoic acid 4 µM and experiment 3 significantly increased in compare to control (P<0.05). The embryonic development rate in the melatonin with 100nM concentration and 4 µM of retinoic acid increased significantly compared to controls (P<0.05). Although, embryonic development rate in experiment 3 was higher than control, but lower in compare to melatonin 100 nM and the retinoic acid 4 µM. The embryonic development rate in experiment 4 significantly increased in compare to control (P<0.05). Conclusion: Combination of melatonin and All-Trans retinoic acid in medium culture increase maturation rate and improved embryonic development in dose dependent manner.http://goums.ac.ir/journal/browse.php?a_code=A-10-1-858&slc_lang=en&sid=1MelatoninRetinoic acidIn-vitro maturationImmature oocytesEmbryonic developmentMaturation rateMouse |
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Tadayoni S Malekzadeh Shafarodi M Ghasemi Hamidabadi H Esmailnejad Moghaddam A Khalilian A Rezaei N |
spellingShingle |
Tadayoni S Malekzadeh Shafarodi M Ghasemi Hamidabadi H Esmailnejad Moghaddam A Khalilian A Rezaei N Effect of combination of Melatonin and All-Trans retinoic acid on maturation, fertilization and embryonic development of immature mouse oocytes مجله دانشگاه علوم پزشکی گرگان Melatonin Retinoic acid In-vitro maturation Immature oocytes Embryonic development Maturation rate Mouse |
author_facet |
Tadayoni S Malekzadeh Shafarodi M Ghasemi Hamidabadi H Esmailnejad Moghaddam A Khalilian A Rezaei N |
author_sort |
Tadayoni S |
title |
Effect of combination of Melatonin and All-Trans retinoic acid on maturation, fertilization and embryonic development of immature mouse oocytes |
title_short |
Effect of combination of Melatonin and All-Trans retinoic acid on maturation, fertilization and embryonic development of immature mouse oocytes |
title_full |
Effect of combination of Melatonin and All-Trans retinoic acid on maturation, fertilization and embryonic development of immature mouse oocytes |
title_fullStr |
Effect of combination of Melatonin and All-Trans retinoic acid on maturation, fertilization and embryonic development of immature mouse oocytes |
title_full_unstemmed |
Effect of combination of Melatonin and All-Trans retinoic acid on maturation, fertilization and embryonic development of immature mouse oocytes |
title_sort |
effect of combination of melatonin and all-trans retinoic acid on maturation, fertilization and embryonic development of immature mouse oocytes |
publisher |
Golestan University of Medical Sciences |
series |
مجله دانشگاه علوم پزشکی گرگان |
issn |
1562-4765 2008-4080 |
publishDate |
2015-09-01 |
description |
Background and Objective: With respect to the antioxidant role of melatonin and retinoic acid, it seems to be effective both in the maturation and embryonic development. This study was done to investigate the effect of combination of melatonin and All-Trans retinoic acid (RA) on maturation, fertilization and embryonic development of immature mouse oocytes. Methods: In this experimental study, cumulus - oocyte complex (COCs) were recovered from 4-6 week old female mice NMRI and were divided into 6 maturation medium groups including control, sham, experiment 1(melatonin 100 nM, 1 and 2 µM), experiment 2 (retinoic acid 1, 2, 4, 6 µM), experiment 3 (melatonin 2 µM+RA 4 µM), experiment 4 (Mel 100nM + retinoic acid 4µM). The maturation rate was recorded after 24 hours of culture in a humidified atmosphere of 5% CO2 at 37°C. The matured oocytes were fertilized with sperm. Fertilization and embryonic development rates to the blastocyst stage were recorded. Results: Maturation rate in the control and sham groups were 50.6% and 49.4%, respectively. Maturation rate were 54.3%, 54.8%, 59.9% in melatonin group with concentrations of 100 nM, 1 and 2 µM, respectively. Maturation rate were 51.6%, 51%, 59% and 49.6% in t-RA group with concentrations of 1, 2, 4, 6 μM. Maturation rate were 60.4% and 54.2% in the experiment 3 and 4 groups, respectively. The maturation rates in the melatonin 2 µM, retinoic acid 4 µM and experiment 3 significantly increased in compare to control (P<0.05). The embryonic development rate in the melatonin with 100nM concentration and 4 µM of retinoic acid increased significantly compared to controls (P<0.05). Although, embryonic development rate in experiment 3 was higher than control, but lower in compare to melatonin 100 nM and the retinoic acid 4 µM. The embryonic development rate in experiment 4 significantly increased in compare to control (P<0.05). Conclusion: Combination of melatonin and All-Trans retinoic acid in medium culture increase maturation rate and improved embryonic development in dose dependent manner. |
topic |
Melatonin Retinoic acid In-vitro maturation Immature oocytes Embryonic development Maturation rate Mouse |
url |
http://goums.ac.ir/journal/browse.php?a_code=A-10-1-858&slc_lang=en&sid=1 |
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