Caraway yellows virus, a novel nepovirus from Carum carvi
Abstract A novel nepovirus was identified and characterised from caraway, and tentatively named caraway yellows virus (CawYV). Tubular structures with isomeric virus particles typical for nepoviruses were observed in infected tissues by electron microscopy. The whole genome of CawYV was identified b...
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doaj-573357e2d6d8401e8f6100ab882789202020-11-25T03:11:28ZengBMCVirology Journal1743-422X2019-05-011611610.1186/s12985-019-1181-1Caraway yellows virus, a novel nepovirus from Carum carviYahya Z. A. Gaafar0Katja R. Richert-Pöggeler1Angelika Sieg-Müller2Petra Lüddecke3Kerstin Herz4Jonas Hartrick5Christina Maaß6Roswitha Ulrich7Heiko Ziebell8Julius Kühn Institute, Institute for Epidemiology and Pathogen DiagnosticsJulius Kühn Institute, Institute for Epidemiology and Pathogen DiagnosticsJulius Kühn Institute, Institute for Epidemiology and Pathogen DiagnosticsJulius Kühn Institute, Institute for Epidemiology and Pathogen DiagnosticsJulius Kühn Institute, Institute for Epidemiology and Pathogen DiagnosticsJulius Kühn Institute, Institute for Epidemiology and Pathogen DiagnosticsJulius Kühn Institute, Institute for Epidemiology and Pathogen DiagnosticsRegierungspräsidium GießenJulius Kühn Institute, Institute for Epidemiology and Pathogen DiagnosticsAbstract A novel nepovirus was identified and characterised from caraway, and tentatively named caraway yellows virus (CawYV). Tubular structures with isomeric virus particles typical for nepoviruses were observed in infected tissues by electron microscopy. The whole genome of CawYV was identified by high throughput sequencing (HTS). It consists of two segments with 8026 nt for RNA1 and 6405 nt for RNA2, excluding the poly(A) tails. CawYV-RNA1 shared closest nt identity to peach rosette mosaic virus (PRMV) with 63%, while RNA2 shared 41.5% with blueberry latent spherical virus (BLSV). The amino acid sequences of the CawYV protease-polymerase (Pro-Pol) and capsid protein (CP) regions share the highest identities with those of the subgroup C nepoviruses. The Pro-Pol region shared highest aa identity with PRMV (80.1%), while the CP region shared 39.6% to soybean latent spherical virus. Phylogenetic analysis of the CawYV-Pro-Pol and -CP aa sequences provided additional evidence of their association with nepoviruses subgroup C. Based on particle morphology, genomic organization and phylogenetic analyses, we propose CawYV as a novel species within the genus Nepovirus subgroup C.http://link.springer.com/article/10.1186/s12985-019-1181-1CarawayHigh throughput sequencingBipartite genomeTubular structuresNepovirus subgroup C |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yahya Z. A. Gaafar Katja R. Richert-Pöggeler Angelika Sieg-Müller Petra Lüddecke Kerstin Herz Jonas Hartrick Christina Maaß Roswitha Ulrich Heiko Ziebell |
spellingShingle |
Yahya Z. A. Gaafar Katja R. Richert-Pöggeler Angelika Sieg-Müller Petra Lüddecke Kerstin Herz Jonas Hartrick Christina Maaß Roswitha Ulrich Heiko Ziebell Caraway yellows virus, a novel nepovirus from Carum carvi Virology Journal Caraway High throughput sequencing Bipartite genome Tubular structures Nepovirus subgroup C |
author_facet |
Yahya Z. A. Gaafar Katja R. Richert-Pöggeler Angelika Sieg-Müller Petra Lüddecke Kerstin Herz Jonas Hartrick Christina Maaß Roswitha Ulrich Heiko Ziebell |
author_sort |
Yahya Z. A. Gaafar |
title |
Caraway yellows virus, a novel nepovirus from Carum carvi |
title_short |
Caraway yellows virus, a novel nepovirus from Carum carvi |
title_full |
Caraway yellows virus, a novel nepovirus from Carum carvi |
title_fullStr |
Caraway yellows virus, a novel nepovirus from Carum carvi |
title_full_unstemmed |
Caraway yellows virus, a novel nepovirus from Carum carvi |
title_sort |
caraway yellows virus, a novel nepovirus from carum carvi |
publisher |
BMC |
series |
Virology Journal |
issn |
1743-422X |
publishDate |
2019-05-01 |
description |
Abstract A novel nepovirus was identified and characterised from caraway, and tentatively named caraway yellows virus (CawYV). Tubular structures with isomeric virus particles typical for nepoviruses were observed in infected tissues by electron microscopy. The whole genome of CawYV was identified by high throughput sequencing (HTS). It consists of two segments with 8026 nt for RNA1 and 6405 nt for RNA2, excluding the poly(A) tails. CawYV-RNA1 shared closest nt identity to peach rosette mosaic virus (PRMV) with 63%, while RNA2 shared 41.5% with blueberry latent spherical virus (BLSV). The amino acid sequences of the CawYV protease-polymerase (Pro-Pol) and capsid protein (CP) regions share the highest identities with those of the subgroup C nepoviruses. The Pro-Pol region shared highest aa identity with PRMV (80.1%), while the CP region shared 39.6% to soybean latent spherical virus. Phylogenetic analysis of the CawYV-Pro-Pol and -CP aa sequences provided additional evidence of their association with nepoviruses subgroup C. Based on particle morphology, genomic organization and phylogenetic analyses, we propose CawYV as a novel species within the genus Nepovirus subgroup C. |
topic |
Caraway High throughput sequencing Bipartite genome Tubular structures Nepovirus subgroup C |
url |
http://link.springer.com/article/10.1186/s12985-019-1181-1 |
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