Arabidopsis mutants may represent recombinant introgression lines
Abstract Objectives It is a common practice in Arabidopsis to transfer a mutation generated in one genetic background to other genetic background via crossing. However, the drawback of this methodology is unavoidable presence of genomic fragments from the donor parent being often replacing desirable...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2018-04-01
|
Series: | BMC Research Notes |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s13104-018-3326-5 |
id |
doaj-57340a212f854e9897b63ab274a2061d |
---|---|
record_format |
Article |
spelling |
doaj-57340a212f854e9897b63ab274a2061d2020-11-24T21:37:01ZengBMCBMC Research Notes1756-05002018-04-011111510.1186/s13104-018-3326-5Arabidopsis mutants may represent recombinant introgression linesNarendra Singh Yadav0Janardan Khadka1Gideon Grafi2French Associates Institute for Agriculture and Biotechnology of Drylands, Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the NegevFrench Associates Institute for Agriculture and Biotechnology of Drylands, Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the NegevFrench Associates Institute for Agriculture and Biotechnology of Drylands, Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the NegevAbstract Objectives It is a common practice in Arabidopsis to transfer a mutation generated in one genetic background to other genetic background via crossing. However, the drawback of this methodology is unavoidable presence of genomic fragments from the donor parent being often replacing desirable genomic fragments of the recurrent parent. Here, we highlighted problem of Arabidopsis mutants being recombinant introgression lines that can lead to unreliable and misinterpreted results. Results We studied the regulation of low copy number transposable elements Tag1 and Evelknievel (EK), located at the end of the bottom arm of chromosome 1 and both are present in the Arabidopsis Landsberg erecta (Ler) but not in Columbia (Col) ecotype. Using various epigenetic mutants (cmt3, ddm1, kyp2, ago4, rdr2 hen1 etc.), we found that certain mutants in the Ler background are deficient of Tag1 or EK or both and represent recombinant introgression lines whereby chromosomal regions from Col have been recombined into the Ler genome. Our data support a recent proposal calling for formulating standards for authentication of plant lines that are used in plant research. Most important is to verify that a given trait or genomic locus under study is correctly identified, particularly when using mutants generated by crossing.http://link.springer.com/article/10.1186/s13104-018-3326-5Arabidopsis thalianaLer ecotypeColumbia ecotypeEvelknievel retroelementTag1 transposable elementcmt3 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Narendra Singh Yadav Janardan Khadka Gideon Grafi |
spellingShingle |
Narendra Singh Yadav Janardan Khadka Gideon Grafi Arabidopsis mutants may represent recombinant introgression lines BMC Research Notes Arabidopsis thaliana Ler ecotype Columbia ecotype Evelknievel retroelement Tag1 transposable element cmt3 |
author_facet |
Narendra Singh Yadav Janardan Khadka Gideon Grafi |
author_sort |
Narendra Singh Yadav |
title |
Arabidopsis mutants may represent recombinant introgression lines |
title_short |
Arabidopsis mutants may represent recombinant introgression lines |
title_full |
Arabidopsis mutants may represent recombinant introgression lines |
title_fullStr |
Arabidopsis mutants may represent recombinant introgression lines |
title_full_unstemmed |
Arabidopsis mutants may represent recombinant introgression lines |
title_sort |
arabidopsis mutants may represent recombinant introgression lines |
publisher |
BMC |
series |
BMC Research Notes |
issn |
1756-0500 |
publishDate |
2018-04-01 |
description |
Abstract Objectives It is a common practice in Arabidopsis to transfer a mutation generated in one genetic background to other genetic background via crossing. However, the drawback of this methodology is unavoidable presence of genomic fragments from the donor parent being often replacing desirable genomic fragments of the recurrent parent. Here, we highlighted problem of Arabidopsis mutants being recombinant introgression lines that can lead to unreliable and misinterpreted results. Results We studied the regulation of low copy number transposable elements Tag1 and Evelknievel (EK), located at the end of the bottom arm of chromosome 1 and both are present in the Arabidopsis Landsberg erecta (Ler) but not in Columbia (Col) ecotype. Using various epigenetic mutants (cmt3, ddm1, kyp2, ago4, rdr2 hen1 etc.), we found that certain mutants in the Ler background are deficient of Tag1 or EK or both and represent recombinant introgression lines whereby chromosomal regions from Col have been recombined into the Ler genome. Our data support a recent proposal calling for formulating standards for authentication of plant lines that are used in plant research. Most important is to verify that a given trait or genomic locus under study is correctly identified, particularly when using mutants generated by crossing. |
topic |
Arabidopsis thaliana Ler ecotype Columbia ecotype Evelknievel retroelement Tag1 transposable element cmt3 |
url |
http://link.springer.com/article/10.1186/s13104-018-3326-5 |
work_keys_str_mv |
AT narendrasinghyadav arabidopsismutantsmayrepresentrecombinantintrogressionlines AT janardankhadka arabidopsismutantsmayrepresentrecombinantintrogressionlines AT gideongrafi arabidopsismutantsmayrepresentrecombinantintrogressionlines |
_version_ |
1725938665629155328 |