Investigating the role of dachshund b in the development of the pancreatic islet in zebrafish
Abstract Aims/Introduction β‐Cell dysfunction is a hallmark of type 2 diabetes. In a previous pilot study, we identified an association between genetic variants within the human DACH1 gene and young‐onset type 2 diabetes. Here, we characterized the function of dachb, the only dach homologue to be ex...
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doaj-574a2acb067d4d3c95dd1ca1f3fb8d1b2021-05-03T02:06:58ZengWileyJournal of Diabetes Investigation2040-11162040-11242021-05-0112571072710.1111/jdi.13503Investigating the role of dachshund b in the development of the pancreatic islet in zebrafishLingling Yang0Sarah E Webb1Nana Jin2Heung Man Lee3Ting Fung Chan4Gang Xu5Juliana CN Chan6Andrew L Miller7Ronald CW Ma8Department of Medicine and Therapeutics The Chinese University of Hong Kong Prince of Wales Hospital Shatin Hong KongDivision of Life Science and State Key Laboratory of Molecular Neuroscience The Hong Kong University of Science and Technology Clear Water Bay Hong KongSchool of Life Sciences and State Key Laboratory of Agrobiotechnology The Chinese University of Hong Kong Shatin Hong KongDepartment of Medicine and Therapeutics The Chinese University of Hong Kong Prince of Wales Hospital Shatin Hong KongSchool of Life Sciences and State Key Laboratory of Agrobiotechnology The Chinese University of Hong Kong Shatin Hong KongDepartment of Medicine and Therapeutics The Chinese University of Hong Kong Prince of Wales Hospital Shatin Hong KongDepartment of Medicine and Therapeutics The Chinese University of Hong Kong Prince of Wales Hospital Shatin Hong KongDivision of Life Science and State Key Laboratory of Molecular Neuroscience The Hong Kong University of Science and Technology Clear Water Bay Hong KongDepartment of Medicine and Therapeutics The Chinese University of Hong Kong Prince of Wales Hospital Shatin Hong KongAbstract Aims/Introduction β‐Cell dysfunction is a hallmark of type 2 diabetes. In a previous pilot study, we identified an association between genetic variants within the human DACH1 gene and young‐onset type 2 diabetes. Here, we characterized the function of dachb, the only dach homologue to be expressed in the pancreas, in developing zebrafish embryos. Materials and Methods We injected one‐cell stage embryos with a dachb‐morpholino (MO) or with the dachb‐MO and dachb messenger ribonucleic acid, and determined the effect on the development of the pancreatic islet. We also carried out quantitative polymerase chain reaction and ribonucleic acid sequencing on the dachb‐MO group to determine the effect of dachb knockdown on gene expression. Results MO‐mediated dachb knockdown resulted in impaired islet cell development, with a significant decrease in both the β‐cell and islet cell numbers. This islet developmental defect was rescued when embryos were co‐injected with dachb‐MO and dachb messenger ribonucleic acid. Knockdown of dachb was associated with a significant downregulation of the β‐cell specific marker gene, insa, and the somatostatin cell marker, sst2, as well as regulators of pancreas development, ptf1a, neuroD, pax6a and nkx6.1, and the cell cycle gene, insm1a. Furthermore, ribonucleic sequencing analysis showed an upregulation of genes enriched in the forkhead box O and mitogen‐activated protein kinase signaling pathways in the dachb‐MO group, when compared with the control groups. Conclusions Together, our results suggest the possible role of dachb in islet development in zebrafish.https://doi.org/10.1111/jdi.13503dachshund bPancreatic islet developmentZebrafish |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lingling Yang Sarah E Webb Nana Jin Heung Man Lee Ting Fung Chan Gang Xu Juliana CN Chan Andrew L Miller Ronald CW Ma |
spellingShingle |
Lingling Yang Sarah E Webb Nana Jin Heung Man Lee Ting Fung Chan Gang Xu Juliana CN Chan Andrew L Miller Ronald CW Ma Investigating the role of dachshund b in the development of the pancreatic islet in zebrafish Journal of Diabetes Investigation dachshund b Pancreatic islet development Zebrafish |
author_facet |
Lingling Yang Sarah E Webb Nana Jin Heung Man Lee Ting Fung Chan Gang Xu Juliana CN Chan Andrew L Miller Ronald CW Ma |
author_sort |
Lingling Yang |
title |
Investigating the role of dachshund b in the development of the pancreatic islet in zebrafish |
title_short |
Investigating the role of dachshund b in the development of the pancreatic islet in zebrafish |
title_full |
Investigating the role of dachshund b in the development of the pancreatic islet in zebrafish |
title_fullStr |
Investigating the role of dachshund b in the development of the pancreatic islet in zebrafish |
title_full_unstemmed |
Investigating the role of dachshund b in the development of the pancreatic islet in zebrafish |
title_sort |
investigating the role of dachshund b in the development of the pancreatic islet in zebrafish |
publisher |
Wiley |
series |
Journal of Diabetes Investigation |
issn |
2040-1116 2040-1124 |
publishDate |
2021-05-01 |
description |
Abstract Aims/Introduction β‐Cell dysfunction is a hallmark of type 2 diabetes. In a previous pilot study, we identified an association between genetic variants within the human DACH1 gene and young‐onset type 2 diabetes. Here, we characterized the function of dachb, the only dach homologue to be expressed in the pancreas, in developing zebrafish embryos. Materials and Methods We injected one‐cell stage embryos with a dachb‐morpholino (MO) or with the dachb‐MO and dachb messenger ribonucleic acid, and determined the effect on the development of the pancreatic islet. We also carried out quantitative polymerase chain reaction and ribonucleic acid sequencing on the dachb‐MO group to determine the effect of dachb knockdown on gene expression. Results MO‐mediated dachb knockdown resulted in impaired islet cell development, with a significant decrease in both the β‐cell and islet cell numbers. This islet developmental defect was rescued when embryos were co‐injected with dachb‐MO and dachb messenger ribonucleic acid. Knockdown of dachb was associated with a significant downregulation of the β‐cell specific marker gene, insa, and the somatostatin cell marker, sst2, as well as regulators of pancreas development, ptf1a, neuroD, pax6a and nkx6.1, and the cell cycle gene, insm1a. Furthermore, ribonucleic sequencing analysis showed an upregulation of genes enriched in the forkhead box O and mitogen‐activated protein kinase signaling pathways in the dachb‐MO group, when compared with the control groups. Conclusions Together, our results suggest the possible role of dachb in islet development in zebrafish. |
topic |
dachshund b Pancreatic islet development Zebrafish |
url |
https://doi.org/10.1111/jdi.13503 |
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