Robust Carbonated Structural Color Barcodes with Ultralow Ontology Fluorescence as Biomimic Culture Platform
Photonic crystal (PC) barcodes are a new type of spectrum-encoding microcarriers used in multiplex high-throughput bioassays, such as broad analysis of biomarkers for clinical diagnosis, gene expression, and cell culture. Unfortunately, most of these existing PC barcodes suffered from undesired feat...
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doaj-57607cbeb703420982dc9968ee4a3fd52021-05-24T12:55:32ZengAmerican Association for the Advancement of ScienceResearch2639-52742021-01-01202110.34133/2021/9851609Robust Carbonated Structural Color Barcodes with Ultralow Ontology Fluorescence as Biomimic Culture PlatformPanmiao Liu0Zhongde Mu1Muhuo Ji2Xiaojiang Liu3Hanwen Gu4Yi Peng5Jianjun Yang6Zhuoying Xie7Fuyin Zheng8Department of Anesthesiology,Pain and Perioperative Medicine,The First Affiliated Hospital of Zhengzhou University,Zhengzhou, 450052,ChinaJiangsu Cancer Hospital & Jiangsu Institute of Cancer Research & The Affiliated Cancer Hospital of Nanjing Medical University,Nanjing 210009,ChinaDepartment of Anesthesiology,The Second Affiliated Hospital,Nanjing Medical University,Nanjing,ChinaState Key Laboratory of Bioelectronics,School of Biological Science and Medical Engineering,Southeast University,Nanjing, 210096,ChinaDepartment of Anesthesiology,Pain and Perioperative Medicine,The First Affiliated Hospital of Zhengzhou University,Zhengzhou, 450052,ChinaJiangsu Cancer Hospital & Jiangsu Institute of Cancer Research & The Affiliated Cancer Hospital of Nanjing Medical University,Nanjing 210009,ChinaDepartment of Anesthesiology,Pain and Perioperative Medicine,The First Affiliated Hospital of Zhengzhou University,Zhengzhou, 450052,ChinaState Key Laboratory of Bioelectronics,School of Biological Science and Medical Engineering,Southeast University,Nanjing, 210096,ChinaKey Laboratory for Biomechanics and Mechanobiology,Beijing Advanced Innovation Center for Biomedical Engineering,School of Biological Science and Medical Engineering,Beihang University, Beijing 100083,ChinaPhotonic crystal (PC) barcodes are a new type of spectrum-encoding microcarriers used in multiplex high-throughput bioassays, such as broad analysis of biomarkers for clinical diagnosis, gene expression, and cell culture. Unfortunately, most of these existing PC barcodes suffered from undesired features, including difficult spectrum-signal acquisition, weak mechanical strength, and high ontology fluorescence, which limited their development to real applications. To address these limitations, we report a new type of structural color-encoded PC barcodes. The barcodes are fabricated by the assembly of monodisperse polydopamine- (PDA-) coated silica (PDA@SiO2) nanoparticles using a droplet-based microfluidic technique and followed by pyrolysis of PDA@SiO2 (C@SiO2) barcodes. Because of the templated carbonization of adhesive PDA, the prepared C@SiO2 PC beads were endowed with simultaneous easy-to-identify structural color, high mechanical strength, and ultralow ontology fluorescence. We demonstrated that the structural colored C@SiO2 barcodes not only maintained a high structural stability and good biocompatibility during the coculturing with fibroblasts and tumor cells capture but also achieved an enhanced fluorescent-reading signal-to-noise ratio in the fluorescence-reading detection. These features make the C@SiO2 PC barcodes versatile for expansive application in fluorescence-reading-based multibioassays.http://dx.doi.org/10.34133/2021/9851609 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Panmiao Liu Zhongde Mu Muhuo Ji Xiaojiang Liu Hanwen Gu Yi Peng Jianjun Yang Zhuoying Xie Fuyin Zheng |
spellingShingle |
Panmiao Liu Zhongde Mu Muhuo Ji Xiaojiang Liu Hanwen Gu Yi Peng Jianjun Yang Zhuoying Xie Fuyin Zheng Robust Carbonated Structural Color Barcodes with Ultralow Ontology Fluorescence as Biomimic Culture Platform Research |
author_facet |
Panmiao Liu Zhongde Mu Muhuo Ji Xiaojiang Liu Hanwen Gu Yi Peng Jianjun Yang Zhuoying Xie Fuyin Zheng |
author_sort |
Panmiao Liu |
title |
Robust Carbonated Structural Color Barcodes with Ultralow Ontology Fluorescence as Biomimic Culture Platform |
title_short |
Robust Carbonated Structural Color Barcodes with Ultralow Ontology Fluorescence as Biomimic Culture Platform |
title_full |
Robust Carbonated Structural Color Barcodes with Ultralow Ontology Fluorescence as Biomimic Culture Platform |
title_fullStr |
Robust Carbonated Structural Color Barcodes with Ultralow Ontology Fluorescence as Biomimic Culture Platform |
title_full_unstemmed |
Robust Carbonated Structural Color Barcodes with Ultralow Ontology Fluorescence as Biomimic Culture Platform |
title_sort |
robust carbonated structural color barcodes with ultralow ontology fluorescence as biomimic culture platform |
publisher |
American Association for the Advancement of Science |
series |
Research |
issn |
2639-5274 |
publishDate |
2021-01-01 |
description |
Photonic crystal (PC) barcodes are a new type of spectrum-encoding microcarriers used in multiplex high-throughput bioassays, such as broad analysis of biomarkers for clinical diagnosis, gene expression, and cell culture. Unfortunately, most of these existing PC barcodes suffered from undesired features, including difficult spectrum-signal acquisition, weak mechanical strength, and high ontology fluorescence, which limited their development to real applications. To address these limitations, we report a new type of structural color-encoded PC barcodes. The barcodes are fabricated by the assembly of monodisperse polydopamine- (PDA-) coated silica (PDA@SiO2) nanoparticles using a droplet-based microfluidic technique and followed by pyrolysis of PDA@SiO2 (C@SiO2) barcodes. Because of the templated carbonization of adhesive PDA, the prepared C@SiO2 PC beads were endowed with simultaneous easy-to-identify structural color, high mechanical strength, and ultralow ontology fluorescence. We demonstrated that the structural colored C@SiO2 barcodes not only maintained a high structural stability and good biocompatibility during the coculturing with fibroblasts and tumor cells capture but also achieved an enhanced fluorescent-reading signal-to-noise ratio in the fluorescence-reading detection. These features make the C@SiO2 PC barcodes versatile for expansive application in fluorescence-reading-based multibioassays. |
url |
http://dx.doi.org/10.34133/2021/9851609 |
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