Comparative effects of organic and inorganic mercury on in vivo dopamine release in freely moving rats
The present study was carried out in order to compare the effects of administration of organic (methylmercury, MeHg) and inorganic (mercury chloride, HgCl 2 ) forms of mercury on in vivo dopamine (DA) release from rat striatum. Experiments were performed in conscious and freely moving female adult S...
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Associação Brasileira de Divulgação Científica
2007-10-01
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doaj-5769459abceb425a839c5e6b81c8be5d2020-11-24T23:15:49ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X1414-431X2007-10-01401013611365Comparative effects of organic and inorganic mercury on in vivo dopamine release in freely moving ratsL.R.F. FaroK.J.A. RodriguesM.B. SantanaL. VidalM. AlfonsoR. DuránThe present study was carried out in order to compare the effects of administration of organic (methylmercury, MeHg) and inorganic (mercury chloride, HgCl 2 ) forms of mercury on in vivo dopamine (DA) release from rat striatum. Experiments were performed in conscious and freely moving female adult Sprague-Dawley (230-280 g) rats using brain microdialysis coupled to HPLC with electrochemical detection. Perfusion of different concentrations of MeHg or HgCl 2 (2 µL/min for 1 h, N = 5-7/group) into the striatum produced significant increases in the levels of DA. Infusion of 40 µM, 400 µM, or 4 mM MeHg increased DA levels to 907 ± 31, 2324 ± 156, and 9032 ± 70% of basal levels, respectively. The same concentrations of HgCl 2 increased DA levels to 1240 ± 66, 2500 ± 424, and 2658 ± 337% of basal levels, respectively. These increases were associated with significant decreases in levels of dihydroxyphenylacetic acid and homovallinic acid. Intrastriatal administration of MeHg induced a sharp concentration-dependent increase in DA levels with a peak 30 min after injection, whereas HgCl 2 induced a gradual, lower (for 4 mM) and delayed increase in DA levels (75 min after the beginning of perfusion). Comparing the neurochemical profile of the two mercury derivatives to induce increases in DA levels, we observed that the time-course of these increases induced by both mercurials was different and the effect produced by HgCl 2 was not concentration-dependent (the effect was the same for the concentrations of 400 µM and 4 mM HgCl 2 ). These results indicate that HgCl 2 produces increases in extracellular DA levels by a mechanism differing from that of MeHg.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001000009MethylmercuryMercury chlorideDopamine release in vivoMicrodialysisMercury determinationStriatum |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
L.R.F. Faro K.J.A. Rodrigues M.B. Santana L. Vidal M. Alfonso R. Durán |
spellingShingle |
L.R.F. Faro K.J.A. Rodrigues M.B. Santana L. Vidal M. Alfonso R. Durán Comparative effects of organic and inorganic mercury on in vivo dopamine release in freely moving rats Brazilian Journal of Medical and Biological Research Methylmercury Mercury chloride Dopamine release in vivo Microdialysis Mercury determination Striatum |
author_facet |
L.R.F. Faro K.J.A. Rodrigues M.B. Santana L. Vidal M. Alfonso R. Durán |
author_sort |
L.R.F. Faro |
title |
Comparative effects of organic and inorganic mercury on in vivo dopamine release in freely moving rats |
title_short |
Comparative effects of organic and inorganic mercury on in vivo dopamine release in freely moving rats |
title_full |
Comparative effects of organic and inorganic mercury on in vivo dopamine release in freely moving rats |
title_fullStr |
Comparative effects of organic and inorganic mercury on in vivo dopamine release in freely moving rats |
title_full_unstemmed |
Comparative effects of organic and inorganic mercury on in vivo dopamine release in freely moving rats |
title_sort |
comparative effects of organic and inorganic mercury on in vivo dopamine release in freely moving rats |
publisher |
Associação Brasileira de Divulgação Científica |
series |
Brazilian Journal of Medical and Biological Research |
issn |
0100-879X 1414-431X |
publishDate |
2007-10-01 |
description |
The present study was carried out in order to compare the effects of administration of organic (methylmercury, MeHg) and inorganic (mercury chloride, HgCl 2 ) forms of mercury on in vivo dopamine (DA) release from rat striatum. Experiments were performed in conscious and freely moving female adult Sprague-Dawley (230-280 g) rats using brain microdialysis coupled to HPLC with electrochemical detection. Perfusion of different concentrations of MeHg or HgCl 2 (2 µL/min for 1 h, N = 5-7/group) into the striatum produced significant increases in the levels of DA. Infusion of 40 µM, 400 µM, or 4 mM MeHg increased DA levels to 907 ± 31, 2324 ± 156, and 9032 ± 70% of basal levels, respectively. The same concentrations of HgCl 2 increased DA levels to 1240 ± 66, 2500 ± 424, and 2658 ± 337% of basal levels, respectively. These increases were associated with significant decreases in levels of dihydroxyphenylacetic acid and homovallinic acid. Intrastriatal administration of MeHg induced a sharp concentration-dependent increase in DA levels with a peak 30 min after injection, whereas HgCl 2 induced a gradual, lower (for 4 mM) and delayed increase in DA levels (75 min after the beginning of perfusion). Comparing the neurochemical profile of the two mercury derivatives to induce increases in DA levels, we observed that the time-course of these increases induced by both mercurials was different and the effect produced by HgCl 2 was not concentration-dependent (the effect was the same for the concentrations of 400 µM and 4 mM HgCl 2 ). These results indicate that HgCl 2 produces increases in extracellular DA levels by a mechanism differing from that of MeHg. |
topic |
Methylmercury Mercury chloride Dopamine release in vivo Microdialysis Mercury determination Striatum |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001000009 |
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