The ER–Golgi intermediate compartment is a key membrane source for the LC3 lipidation step of autophagosome biogenesis
Autophagy is a catabolic process for bulk degradation of cytosolic materials mediated by double-membraned autophagosomes. The membrane determinant to initiate the formation of autophagosomes remains elusive. Here, we establish a cell-free assay based on LC3 lipidation to define the organelle membran...
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doaj-57780319853c45529fb4039188c0715f2021-05-04T22:30:24ZengeLife Sciences Publications LtdeLife2050-084X2013-08-01210.7554/eLife.00947The ER–Golgi intermediate compartment is a key membrane source for the LC3 lipidation step of autophagosome biogenesisLiang Ge0David Melville1Min Zhang2Randy Schekman3Department of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, United StatesDepartment of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, United StatesDepartment of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, United StatesDepartment of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, United StatesAutophagy is a catabolic process for bulk degradation of cytosolic materials mediated by double-membraned autophagosomes. The membrane determinant to initiate the formation of autophagosomes remains elusive. Here, we establish a cell-free assay based on LC3 lipidation to define the organelle membrane supporting early autophagosome formation. In vitro LC3 lipidation requires energy and is subject to regulation by the pathways modulating autophagy in vivo. We developed a systematic membrane isolation scheme to identify the endoplasmic reticulum–Golgi intermediate compartment (ERGIC) as a primary membrane source both necessary and sufficient to trigger LC3 lipidation in vitro. Functional studies demonstrate that the ERGIC is required for autophagosome biogenesis in vivo. Moreover, we find that the ERGIC acts by recruiting the early autophagosome marker ATG14, a critical step for the generation of preautophagosomal membranes.https://elifesciences.org/articles/00947autophagyER–Golgi intermediate compartmentLC3 lipidationautophagosome |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Liang Ge David Melville Min Zhang Randy Schekman |
spellingShingle |
Liang Ge David Melville Min Zhang Randy Schekman The ER–Golgi intermediate compartment is a key membrane source for the LC3 lipidation step of autophagosome biogenesis eLife autophagy ER–Golgi intermediate compartment LC3 lipidation autophagosome |
author_facet |
Liang Ge David Melville Min Zhang Randy Schekman |
author_sort |
Liang Ge |
title |
The ER–Golgi intermediate compartment is a key membrane source for the LC3 lipidation step of autophagosome biogenesis |
title_short |
The ER–Golgi intermediate compartment is a key membrane source for the LC3 lipidation step of autophagosome biogenesis |
title_full |
The ER–Golgi intermediate compartment is a key membrane source for the LC3 lipidation step of autophagosome biogenesis |
title_fullStr |
The ER–Golgi intermediate compartment is a key membrane source for the LC3 lipidation step of autophagosome biogenesis |
title_full_unstemmed |
The ER–Golgi intermediate compartment is a key membrane source for the LC3 lipidation step of autophagosome biogenesis |
title_sort |
er–golgi intermediate compartment is a key membrane source for the lc3 lipidation step of autophagosome biogenesis |
publisher |
eLife Sciences Publications Ltd |
series |
eLife |
issn |
2050-084X |
publishDate |
2013-08-01 |
description |
Autophagy is a catabolic process for bulk degradation of cytosolic materials mediated by double-membraned autophagosomes. The membrane determinant to initiate the formation of autophagosomes remains elusive. Here, we establish a cell-free assay based on LC3 lipidation to define the organelle membrane supporting early autophagosome formation. In vitro LC3 lipidation requires energy and is subject to regulation by the pathways modulating autophagy in vivo. We developed a systematic membrane isolation scheme to identify the endoplasmic reticulum–Golgi intermediate compartment (ERGIC) as a primary membrane source both necessary and sufficient to trigger LC3 lipidation in vitro. Functional studies demonstrate that the ERGIC is required for autophagosome biogenesis in vivo. Moreover, we find that the ERGIC acts by recruiting the early autophagosome marker ATG14, a critical step for the generation of preautophagosomal membranes. |
topic |
autophagy ER–Golgi intermediate compartment LC3 lipidation autophagosome |
url |
https://elifesciences.org/articles/00947 |
work_keys_str_mv |
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