Analysis of Chemical Composition and In vitro and In vivo Antifungal Activity of <i>Raphanus raphanistrum </i>Extracts Against <i>Fusarium</i> and <i>Pythiaceae</i>, Affecting Apple and Peach Seedlings
The goal of this investigation was to evaluate the <i>in vitro</i> and <i>in vivo</i> efficiency of <i>Raphanus raphanistrum</i> extracts against <i>Fusarium</i> and <i>Pythiaceae</i>s pecies associated with apple and peach seedling decline...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2021-04-01
|
Series: | Molecules |
Subjects: | |
Online Access: | https://www.mdpi.com/1420-3049/26/9/2479 |
id |
doaj-5797099afa5a4db0b69405d1221453b6 |
---|---|
record_format |
Article |
spelling |
doaj-5797099afa5a4db0b69405d1221453b62021-04-23T23:07:01ZengMDPI AGMolecules1420-30492021-04-01262479247910.3390/molecules26092479Analysis of Chemical Composition and In vitro and In vivo Antifungal Activity of <i>Raphanus raphanistrum </i>Extracts Against <i>Fusarium</i> and <i>Pythiaceae</i>, Affecting Apple and Peach SeedlingsSabrine Mannai0Najwa BenfradjAhlem KarouiIbtissem Ben Ben SalemAmel FathallahMahmoud M’HamdiNaima Boughalleb-M’HamdiDepartment of Biological Sciences and Plant Protection, High Institute of Agronomy of Chott Mariem, University of Sousse, 4042 Sousse, UR13AGR03, TunisiaThe goal of this investigation was to evaluate the <i>in vitro</i> and <i>in vivo</i> efficiency of <i>Raphanus raphanistrum</i> extracts against <i>Fusarium</i> and <i>Pythiaceae</i>s pecies associated with apple and peach seedling decline in Tunisian nurseries. A chemical composition of organic extracts was accomplished using liquid chromatography, thin layer chromatography, and gas chromatography analysis. The <i>in vitro</i> test of three aqueous extract doses of <i>R. raphanistrum</i> against some apple and peach decline agents showed its efficacy in reducing mycelia growth. The <i>in vivo</i> assay of fine powder of this plant on peach seedlings revealed that treatment 8-weeks before the inoculation and planting was more efficient than the treatment before one week. This experiment revealed that the root weight of peach seedlings inoculated by F. oxysporum was improved to 207.29%. For apple seedlings, the treatment 8 weeks before the inoculation and plantation was more efficient than the treatment one week before; it reduced the root browning index. The study of <i>R. raphanistrum</i> chemical composition and its efficiency showed that the glucosinolates products: nitrile (4-Hydroxy-3-(4-methylphenylthio) butane nitrile, benzene acetonitrile, 4-fluoro,butane nitrile, 4-hydroxy-3-[(4-methylphenyl) thio] nitrile), and thiocyanate molecules (thiocyanic acid, ethyle) are responsible for the anti-fungal activities.https://www.mdpi.com/1420-3049/26/9/2479<i>Raphanus raphanistrum</i>aqueousorganic extractsliquid chromatographygas chromatographybiocontrol |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sabrine Mannai Najwa Benfradj Ahlem Karoui Ibtissem Ben Ben Salem Amel Fathallah Mahmoud M’Hamdi Naima Boughalleb-M’Hamdi |
spellingShingle |
Sabrine Mannai Najwa Benfradj Ahlem Karoui Ibtissem Ben Ben Salem Amel Fathallah Mahmoud M’Hamdi Naima Boughalleb-M’Hamdi Analysis of Chemical Composition and In vitro and In vivo Antifungal Activity of <i>Raphanus raphanistrum </i>Extracts Against <i>Fusarium</i> and <i>Pythiaceae</i>, Affecting Apple and Peach Seedlings Molecules <i>Raphanus raphanistrum</i> aqueous organic extracts liquid chromatography gas chromatography biocontrol |
author_facet |
Sabrine Mannai Najwa Benfradj Ahlem Karoui Ibtissem Ben Ben Salem Amel Fathallah Mahmoud M’Hamdi Naima Boughalleb-M’Hamdi |
author_sort |
Sabrine Mannai |
title |
Analysis of Chemical Composition and In vitro and In vivo Antifungal Activity of <i>Raphanus raphanistrum </i>Extracts Against <i>Fusarium</i> and <i>Pythiaceae</i>, Affecting Apple and Peach Seedlings |
title_short |
Analysis of Chemical Composition and In vitro and In vivo Antifungal Activity of <i>Raphanus raphanistrum </i>Extracts Against <i>Fusarium</i> and <i>Pythiaceae</i>, Affecting Apple and Peach Seedlings |
title_full |
Analysis of Chemical Composition and In vitro and In vivo Antifungal Activity of <i>Raphanus raphanistrum </i>Extracts Against <i>Fusarium</i> and <i>Pythiaceae</i>, Affecting Apple and Peach Seedlings |
title_fullStr |
Analysis of Chemical Composition and In vitro and In vivo Antifungal Activity of <i>Raphanus raphanistrum </i>Extracts Against <i>Fusarium</i> and <i>Pythiaceae</i>, Affecting Apple and Peach Seedlings |
title_full_unstemmed |
Analysis of Chemical Composition and In vitro and In vivo Antifungal Activity of <i>Raphanus raphanistrum </i>Extracts Against <i>Fusarium</i> and <i>Pythiaceae</i>, Affecting Apple and Peach Seedlings |
title_sort |
analysis of chemical composition and in vitro and in vivo antifungal activity of <i>raphanus raphanistrum </i>extracts against <i>fusarium</i> and <i>pythiaceae</i>, affecting apple and peach seedlings |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2021-04-01 |
description |
The goal of this investigation was to evaluate the <i>in vitro</i> and <i>in vivo</i> efficiency of <i>Raphanus raphanistrum</i> extracts against <i>Fusarium</i> and <i>Pythiaceae</i>s pecies associated with apple and peach seedling decline in Tunisian nurseries. A chemical composition of organic extracts was accomplished using liquid chromatography, thin layer chromatography, and gas chromatography analysis. The <i>in vitro</i> test of three aqueous extract doses of <i>R. raphanistrum</i> against some apple and peach decline agents showed its efficacy in reducing mycelia growth. The <i>in vivo</i> assay of fine powder of this plant on peach seedlings revealed that treatment 8-weeks before the inoculation and planting was more efficient than the treatment before one week. This experiment revealed that the root weight of peach seedlings inoculated by F. oxysporum was improved to 207.29%. For apple seedlings, the treatment 8 weeks before the inoculation and plantation was more efficient than the treatment one week before; it reduced the root browning index. The study of <i>R. raphanistrum</i> chemical composition and its efficiency showed that the glucosinolates products: nitrile (4-Hydroxy-3-(4-methylphenylthio) butane nitrile, benzene acetonitrile, 4-fluoro,butane nitrile, 4-hydroxy-3-[(4-methylphenyl) thio] nitrile), and thiocyanate molecules (thiocyanic acid, ethyle) are responsible for the anti-fungal activities. |
topic |
<i>Raphanus raphanistrum</i> aqueous organic extracts liquid chromatography gas chromatography biocontrol |
url |
https://www.mdpi.com/1420-3049/26/9/2479 |
work_keys_str_mv |
AT sabrinemannai analysisofchemicalcompositionandinvitroandinvivoantifungalactivityofiraphanusraphanistrumiextractsagainstifusariumiandipythiaceaeiaffectingappleandpeachseedlings AT najwabenfradj analysisofchemicalcompositionandinvitroandinvivoantifungalactivityofiraphanusraphanistrumiextractsagainstifusariumiandipythiaceaeiaffectingappleandpeachseedlings AT ahlemkaroui analysisofchemicalcompositionandinvitroandinvivoantifungalactivityofiraphanusraphanistrumiextractsagainstifusariumiandipythiaceaeiaffectingappleandpeachseedlings AT ibtissembenbensalem analysisofchemicalcompositionandinvitroandinvivoantifungalactivityofiraphanusraphanistrumiextractsagainstifusariumiandipythiaceaeiaffectingappleandpeachseedlings AT amelfathallah analysisofchemicalcompositionandinvitroandinvivoantifungalactivityofiraphanusraphanistrumiextractsagainstifusariumiandipythiaceaeiaffectingappleandpeachseedlings AT mahmoudmhamdi analysisofchemicalcompositionandinvitroandinvivoantifungalactivityofiraphanusraphanistrumiextractsagainstifusariumiandipythiaceaeiaffectingappleandpeachseedlings AT naimaboughallebmhamdi analysisofchemicalcompositionandinvitroandinvivoantifungalactivityofiraphanusraphanistrumiextractsagainstifusariumiandipythiaceaeiaffectingappleandpeachseedlings |
_version_ |
1721511928805720064 |