Cytokine and chemokine levels in the heart tissue of aged rats following severe acute pancreatitis
Severe acute pancreatitis (AP) is a disease associated with high mortality and characterized by overwhelming systemic inflammation. Older people have a prolonged hospital stay and worst prognosis, when affected by this disease. Our group hypothesized, thus, that the systemic inflammatory response in...
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doaj-579e7dbabbe34d8a81375dd8f8d921142020-11-25T02:59:52ZengSAGE PublishingEuropean Journal of Inflammation1721-727X2017-08-011510.1177/1721727X17712398Cytokine and chemokine levels in the heart tissue of aged rats following severe acute pancreatitisRízia Callou Amaral0Denise Frediani Barbeiro1Marcia Kiyomi Koike2Charles Mady3Marcel Cerqueira César Machado4Fabiano Pinheiro da Silva5Emergency Medicine Department, University of São Paulo, São Paulo, BrazilEmergency Medicine Department, University of São Paulo, São Paulo, BrazilEmergency Medicine Department, University of São Paulo, São Paulo, BrazilHeart Institute, University of São Paulo, São Paulo, BrazilEmergency Medicine Department, University of São Paulo, São Paulo, BrazilEmergency Medicine Department, University of São Paulo, São Paulo, BrazilSevere acute pancreatitis (AP) is a disease associated with high mortality and characterized by overwhelming systemic inflammation. Older people have a prolonged hospital stay and worst prognosis, when affected by this disease. Our group hypothesized, thus, that the systemic inflammatory response in the elderly would promote more organ damage when compared to the young. We sought to investigate the effect of systemic inflammation on the gene expression of cytokines, chemokines, and growth factors in the hearts of older and younger rats in an animal model of AP. AP was induced in all rats by injection of 0.5 mL of 2.5% taurocholate. There were two healthy age-matched control groups. An array of 79 cytokines, chemokines, and growth factors was measured in samples of cardiac tissue taken from the AP rats after 10 h, and from control rats. Older healthy rats had significantly higher levels of interleukin-10 (IL-10) and CCL1 gene expression than younger ones ( P < 0.05), but all other measurements were similar among the study groups. This study indicates the systemic inflammation may show unique features for different organs in the body, but older animals with systemic inflammation are similar to the young regarding the cardiac inflammatory response.https://doi.org/10.1177/1721727X17712398 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rízia Callou Amaral Denise Frediani Barbeiro Marcia Kiyomi Koike Charles Mady Marcel Cerqueira César Machado Fabiano Pinheiro da Silva |
spellingShingle |
Rízia Callou Amaral Denise Frediani Barbeiro Marcia Kiyomi Koike Charles Mady Marcel Cerqueira César Machado Fabiano Pinheiro da Silva Cytokine and chemokine levels in the heart tissue of aged rats following severe acute pancreatitis European Journal of Inflammation |
author_facet |
Rízia Callou Amaral Denise Frediani Barbeiro Marcia Kiyomi Koike Charles Mady Marcel Cerqueira César Machado Fabiano Pinheiro da Silva |
author_sort |
Rízia Callou Amaral |
title |
Cytokine and chemokine levels in the heart tissue of aged rats following severe acute pancreatitis |
title_short |
Cytokine and chemokine levels in the heart tissue of aged rats following severe acute pancreatitis |
title_full |
Cytokine and chemokine levels in the heart tissue of aged rats following severe acute pancreatitis |
title_fullStr |
Cytokine and chemokine levels in the heart tissue of aged rats following severe acute pancreatitis |
title_full_unstemmed |
Cytokine and chemokine levels in the heart tissue of aged rats following severe acute pancreatitis |
title_sort |
cytokine and chemokine levels in the heart tissue of aged rats following severe acute pancreatitis |
publisher |
SAGE Publishing |
series |
European Journal of Inflammation |
issn |
1721-727X |
publishDate |
2017-08-01 |
description |
Severe acute pancreatitis (AP) is a disease associated with high mortality and characterized by overwhelming systemic inflammation. Older people have a prolonged hospital stay and worst prognosis, when affected by this disease. Our group hypothesized, thus, that the systemic inflammatory response in the elderly would promote more organ damage when compared to the young. We sought to investigate the effect of systemic inflammation on the gene expression of cytokines, chemokines, and growth factors in the hearts of older and younger rats in an animal model of AP. AP was induced in all rats by injection of 0.5 mL of 2.5% taurocholate. There were two healthy age-matched control groups. An array of 79 cytokines, chemokines, and growth factors was measured in samples of cardiac tissue taken from the AP rats after 10 h, and from control rats. Older healthy rats had significantly higher levels of interleukin-10 (IL-10) and CCL1 gene expression than younger ones ( P < 0.05), but all other measurements were similar among the study groups. This study indicates the systemic inflammation may show unique features for different organs in the body, but older animals with systemic inflammation are similar to the young regarding the cardiac inflammatory response. |
url |
https://doi.org/10.1177/1721727X17712398 |
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