Real-Time Polymerase Chain Reaction for Detecting SARS Coronavirus, Beijing, 2003

During the 2003 severe acute respiratory syndrome (SARS) outbreak, a real-time quantitative polymerase chain reaction, which targets the nucleocapsid gene at the 3′-end of the viral genome, was established to detect and identify the SARS-associated coronavirus. We describe the use of this assay to s...

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Main Authors:	Junhui Zhai, Thomas Briese, Erhei Dai, Xiaoyi Wang, Xin Pang, Zongmin Du, Haihong Liu, Jin Wang, Hongxia Wang, Zhaobiao Guo, Zeliang Chen, Lingxiao Jiang, Dongsheng Zhou, Yanping Han, Omar Jabado, Gustavo Palacios, W. Ian Lipkin, Ruifu Yang
Format:	Article
Language:	English
Published:	Centers for Disease Control and Prevention 2004-02-01
Series:	Emerging Infectious Diseases
Subjects:	SARS-CoV PCR diagnostic China
Online Access:	https://wwwnc.cdc.gov/eid/article/10/2/03-0799_article

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spelling	doaj-57a9e0d04f624dca90bacc1491051ec52020-11-25T02:35:53ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592004-02-0110230030310.3201/eid1002.030799Real-Time Polymerase Chain Reaction for Detecting SARS Coronavirus, Beijing, 2003Junhui ZhaiThomas BrieseErhei DaiXiaoyi WangXin PangZongmin DuHaihong LiuJin WangHongxia WangZhaobiao GuoZeliang ChenLingxiao JiangDongsheng ZhouYanping HanOmar JabadoGustavo PalaciosW. Ian LipkinRuifu YangDuring the 2003 severe acute respiratory syndrome (SARS) outbreak, a real-time quantitative polymerase chain reaction, which targets the nucleocapsid gene at the 3′-end of the viral genome, was established to detect and identify the SARS-associated coronavirus. We describe the use of this assay to screen >700 clinical samples.https://wwwnc.cdc.gov/eid/article/10/2/03-0799_articleSARS-CoVPCRdiagnosticChina
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Junhui Zhai Thomas Briese Erhei Dai Xiaoyi Wang Xin Pang Zongmin Du Haihong Liu Jin Wang Hongxia Wang Zhaobiao Guo Zeliang Chen Lingxiao Jiang Dongsheng Zhou Yanping Han Omar Jabado Gustavo Palacios W. Ian Lipkin Ruifu Yang
spellingShingle	Junhui Zhai Thomas Briese Erhei Dai Xiaoyi Wang Xin Pang Zongmin Du Haihong Liu Jin Wang Hongxia Wang Zhaobiao Guo Zeliang Chen Lingxiao Jiang Dongsheng Zhou Yanping Han Omar Jabado Gustavo Palacios W. Ian Lipkin Ruifu Yang Real-Time Polymerase Chain Reaction for Detecting SARS Coronavirus, Beijing, 2003 Emerging Infectious Diseases SARS-CoV PCR diagnostic China
author_facet	Junhui Zhai Thomas Briese Erhei Dai Xiaoyi Wang Xin Pang Zongmin Du Haihong Liu Jin Wang Hongxia Wang Zhaobiao Guo Zeliang Chen Lingxiao Jiang Dongsheng Zhou Yanping Han Omar Jabado Gustavo Palacios W. Ian Lipkin Ruifu Yang
author_sort	Junhui Zhai
title	Real-Time Polymerase Chain Reaction for Detecting SARS Coronavirus, Beijing, 2003
title_short	Real-Time Polymerase Chain Reaction for Detecting SARS Coronavirus, Beijing, 2003
title_full	Real-Time Polymerase Chain Reaction for Detecting SARS Coronavirus, Beijing, 2003
title_fullStr	Real-Time Polymerase Chain Reaction for Detecting SARS Coronavirus, Beijing, 2003
title_full_unstemmed	Real-Time Polymerase Chain Reaction for Detecting SARS Coronavirus, Beijing, 2003
title_sort	real-time polymerase chain reaction for detecting sars coronavirus, beijing, 2003
publisher	Centers for Disease Control and Prevention
series	Emerging Infectious Diseases
issn	1080-6040 1080-6059
publishDate	2004-02-01
description	During the 2003 severe acute respiratory syndrome (SARS) outbreak, a real-time quantitative polymerase chain reaction, which targets the nucleocapsid gene at the 3′-end of the viral genome, was established to detect and identify the SARS-associated coronavirus. We describe the use of this assay to screen >700 clinical samples.
topic	SARS-CoV PCR diagnostic China
url	https://wwwnc.cdc.gov/eid/article/10/2/03-0799_article
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Real-Time Polymerase Chain Reaction for Detecting SARS Coronavirus, Beijing, 2003

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