STRUCTURAL CHANGES IN THE RAT TESTIS CAUSED BY DIAZINON AND SELENIUM
<div style="mso-element: frame; mso-element-frame-width: 498.1pt; mso-element-frame-height: 318.1pt; mso-element-frame-vspace: 3.1pt; mso-element-wrap: around; mso-element-anchor-vertical: page; mso-element-anchor-horizontal: page; mso-element-left: 52.45pt; mso-element-top: 55.2pt; mso-heig...
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doaj-57bfa7594d824e6685004c1f7ae4bba62020-11-25T00:45:19ZengHACCP ConsultingPotravinarstvo 1338-02301337-09602010-05-014281610.5219/44STRUCTURAL CHANGES IN THE RAT TESTIS CAUSED BY DIAZINON AND SELENIUMJozef GolianNorbert LukáčBranislav ŠiškaPeter MassányiMária AdamkovičováRóbert TomanMichal Cabaj<div style="mso-element: frame; mso-element-frame-width: 498.1pt; mso-element-frame-height: 318.1pt; mso-element-frame-vspace: 3.1pt; mso-element-wrap: around; mso-element-anchor-vertical: page; mso-element-anchor-horizontal: page; mso-element-left: 52.45pt; mso-element-top: 55.2pt; mso-height-rule: exactly;"><span style="font-size: medium;"><p class="MsoNormal">The aim of this study was to find the structural changes in the rat testis after a diazinon, selenium and their combined administration. The testis structural changes after the diazinon intraperitoneal administration of 20 mg/kg b.w., selenium 2 mg/kg b.w. and both diazinon 20 mg/kg b.w. and selenium 2 mg/kg b.w. were evaluated by histological and morphometric methods in light microscopy. 36 hours after the diazion i.p. administration, the vacuolization of the seminiferous epithelium, evacuation of germ cells into the tubule lumen, epithelium necrosis and disintegration, interstitium extension and fibrotization were observed. The germ cells released from the basal lamina and subsequently they were visible in the tubule lumen. Blood vessels were damaged and morphometric analysis have shown their significant dilatation (P<0.05). Diazinon causes the damage of the germinal epithelium in the testes leading to the spermatogenesis failure. The infertility can then appear. In selenium treated group disintegration of cellular associations in the seminiferous epithelium, damaged and separating spermatids lines, reduced spermatogenesis and significant vacuolization of seminiferous epithelium (P<0.0001) were observed. Similar changes as in selenium threated group occured, significant vacuolization of seminiferous epithelium (P<0.05) was seen. In combined diazinon and selenium treated group protective effects of selenium in seminiferous epithelium and interstitium were noticed. Further investigation of diazinon and selenium is needed to practical use this results.</p><p class="MsoNormal"> </p><p class="MsoNormal"><span style="font-size: 12px;"><strong>doi:10.5219/44</strong></span></p> <p class="MsoNormal"> </p></span></div>http://www.potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/44diazinon, selenium, testis, histology, morphometry, rat |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jozef Golian Norbert Lukáč Branislav Šiška Peter Massányi Mária Adamkovičová Róbert Toman Michal Cabaj |
spellingShingle |
Jozef Golian Norbert Lukáč Branislav Šiška Peter Massányi Mária Adamkovičová Róbert Toman Michal Cabaj STRUCTURAL CHANGES IN THE RAT TESTIS CAUSED BY DIAZINON AND SELENIUM Potravinarstvo diazinon, selenium, testis, histology, morphometry, rat |
author_facet |
Jozef Golian Norbert Lukáč Branislav Šiška Peter Massányi Mária Adamkovičová Róbert Toman Michal Cabaj |
author_sort |
Jozef Golian |
title |
STRUCTURAL CHANGES IN THE RAT TESTIS CAUSED BY DIAZINON AND SELENIUM |
title_short |
STRUCTURAL CHANGES IN THE RAT TESTIS CAUSED BY DIAZINON AND SELENIUM |
title_full |
STRUCTURAL CHANGES IN THE RAT TESTIS CAUSED BY DIAZINON AND SELENIUM |
title_fullStr |
STRUCTURAL CHANGES IN THE RAT TESTIS CAUSED BY DIAZINON AND SELENIUM |
title_full_unstemmed |
STRUCTURAL CHANGES IN THE RAT TESTIS CAUSED BY DIAZINON AND SELENIUM |
title_sort |
structural changes in the rat testis caused by diazinon and selenium |
publisher |
HACCP Consulting |
series |
Potravinarstvo |
issn |
1338-0230 1337-0960 |
publishDate |
2010-05-01 |
description |
<div style="mso-element: frame; mso-element-frame-width: 498.1pt; mso-element-frame-height: 318.1pt; mso-element-frame-vspace: 3.1pt; mso-element-wrap: around; mso-element-anchor-vertical: page; mso-element-anchor-horizontal: page; mso-element-left: 52.45pt; mso-element-top: 55.2pt; mso-height-rule: exactly;"><span style="font-size: medium;"><p class="MsoNormal">The aim of this study was to find the structural changes in the rat testis after a diazinon, selenium and their combined administration. The testis structural changes after the diazinon intraperitoneal administration of 20 mg/kg b.w., selenium 2 mg/kg b.w. and both diazinon 20 mg/kg b.w. and selenium 2 mg/kg b.w. were evaluated by histological and morphometric methods in light microscopy. 36 hours after the diazion i.p. administration, the vacuolization of the seminiferous epithelium, evacuation of germ cells into the tubule lumen, epithelium necrosis and disintegration, interstitium extension and fibrotization were observed. The germ cells released from the basal lamina and subsequently they were visible in the tubule lumen. Blood vessels were damaged and morphometric analysis have shown their significant dilatation (P<0.05). Diazinon causes the damage of the germinal epithelium in the testes leading to the spermatogenesis failure. The infertility can then appear. In selenium treated group disintegration of cellular associations in the seminiferous epithelium, damaged and separating spermatids lines, reduced spermatogenesis and significant vacuolization of seminiferous epithelium (P<0.0001) were observed. Similar changes as in selenium threated group occured, significant vacuolization of seminiferous epithelium (P<0.05) was seen. In combined diazinon and selenium treated group protective effects of selenium in seminiferous epithelium and interstitium were noticed. Further investigation of diazinon and selenium is needed to practical use this results.</p><p class="MsoNormal"> </p><p class="MsoNormal"><span style="font-size: 12px;"><strong>doi:10.5219/44</strong></span></p> <p class="MsoNormal"> </p></span></div> |
topic |
diazinon, selenium, testis, histology, morphometry, rat |
url |
http://www.potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/44 |
work_keys_str_mv |
AT jozefgolian structuralchangesintherattestiscausedbydiazinonandselenium AT norbertlukac structuralchangesintherattestiscausedbydiazinonandselenium AT branislavsiska structuralchangesintherattestiscausedbydiazinonandselenium AT petermassanyi structuralchangesintherattestiscausedbydiazinonandselenium AT mariaadamkovicova structuralchangesintherattestiscausedbydiazinonandselenium AT roberttoman structuralchangesintherattestiscausedbydiazinonandselenium AT michalcabaj structuralchangesintherattestiscausedbydiazinonandselenium |
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1725270880885407744 |