Mammalian cell entry operons; novel and major subset candidates for diagnostics with special reference to Mycobacterium avium subspecies paratuberculosis infection
Mammalian cell entry (mce) genes are the components of the mce operon and play a vital role in the entry of Mycobacteria into the mammalian cell and their survival within phagocytes and epithelial cells. Mce operons are present in the DNA of Mycobacteria and translate proteins associated with the in...
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doaj-57d558252fa341409c8618de31d48e2a2020-11-25T01:48:50ZengTaylor & Francis GroupVeterinary Quarterly0165-21761875-59412019-01-01391657510.1080/01652176.2019.16417641641764Mammalian cell entry operons; novel and major subset candidates for diagnostics with special reference to Mycobacterium avium subspecies paratuberculosis infectionZahra Hemati0Abdollah Derakhshandeh1Masoud Haghkhah2Kundan Kumar Chaubey3Saurabh Gupta4Manju Singh5Shoorvir V. Singh6Kuldeep Dhama7Shiraz UniversityShiraz UniversityShiraz UniversityGLA UniversityGLA UniversityGLA UniversityGLA UniversityIndian Veterinary Research InstituteMammalian cell entry (mce) genes are the components of the mce operon and play a vital role in the entry of Mycobacteria into the mammalian cell and their survival within phagocytes and epithelial cells. Mce operons are present in the DNA of Mycobacteria and translate proteins associated with the invasion and long-term existence of these pathogens in macrophages. The exact mechanism of action of mce genes and their functions are not clear yet. However, with the loss of these genes Mycobacteria lose their pathogenicity. Mycobacterium avium subspecies paratuberculosis (MAP), the etiological agent of Johne’s disease, is the cause of chronic enteritis of animals and significantly affects economic impact on the livestock industry. Since MAP is not inactivated during pasteurization, human population is continuously at the risk of getting exposed to MAP infection through consumption of dairy products. There is need for new candidate genes and/or proteins for developing improved diagnostic assays for the diagnosis of MAP infection and for the control of disease. Increasing evidences showed that expression of mce genes is important for the virulence of MAP. Whole-genome DNA microarray representing MAP revealed that there are 14 large sequence polymorphisms with LSPP12 being the most widely conserved MAP-specific region that included a cluster of six homologs of mce-family involved in lipid metabolism. On the other hand, LSP11 comprising part of mce2 operon was absent in MAP isolates. This review summarizes the advancement of research on mce genes of Mycobacteria with special reference to the MAP infection.http://dx.doi.org/10.1080/01652176.2019.1641764mce operonmycobacteriaproteindiagnosismapmtbjohne’s disease |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zahra Hemati Abdollah Derakhshandeh Masoud Haghkhah Kundan Kumar Chaubey Saurabh Gupta Manju Singh Shoorvir V. Singh Kuldeep Dhama |
spellingShingle |
Zahra Hemati Abdollah Derakhshandeh Masoud Haghkhah Kundan Kumar Chaubey Saurabh Gupta Manju Singh Shoorvir V. Singh Kuldeep Dhama Mammalian cell entry operons; novel and major subset candidates for diagnostics with special reference to Mycobacterium avium subspecies paratuberculosis infection Veterinary Quarterly mce operon mycobacteria protein diagnosis map mtb johne’s disease |
author_facet |
Zahra Hemati Abdollah Derakhshandeh Masoud Haghkhah Kundan Kumar Chaubey Saurabh Gupta Manju Singh Shoorvir V. Singh Kuldeep Dhama |
author_sort |
Zahra Hemati |
title |
Mammalian cell entry operons; novel and major subset candidates for diagnostics with special reference to Mycobacterium avium subspecies paratuberculosis infection |
title_short |
Mammalian cell entry operons; novel and major subset candidates for diagnostics with special reference to Mycobacterium avium subspecies paratuberculosis infection |
title_full |
Mammalian cell entry operons; novel and major subset candidates for diagnostics with special reference to Mycobacterium avium subspecies paratuberculosis infection |
title_fullStr |
Mammalian cell entry operons; novel and major subset candidates for diagnostics with special reference to Mycobacterium avium subspecies paratuberculosis infection |
title_full_unstemmed |
Mammalian cell entry operons; novel and major subset candidates for diagnostics with special reference to Mycobacterium avium subspecies paratuberculosis infection |
title_sort |
mammalian cell entry operons; novel and major subset candidates for diagnostics with special reference to mycobacterium avium subspecies paratuberculosis infection |
publisher |
Taylor & Francis Group |
series |
Veterinary Quarterly |
issn |
0165-2176 1875-5941 |
publishDate |
2019-01-01 |
description |
Mammalian cell entry (mce) genes are the components of the mce operon and play a vital role in the entry of Mycobacteria into the mammalian cell and their survival within phagocytes and epithelial cells. Mce operons are present in the DNA of Mycobacteria and translate proteins associated with the invasion and long-term existence of these pathogens in macrophages. The exact mechanism of action of mce genes and their functions are not clear yet. However, with the loss of these genes Mycobacteria lose their pathogenicity. Mycobacterium avium subspecies paratuberculosis (MAP), the etiological agent of Johne’s disease, is the cause of chronic enteritis of animals and significantly affects economic impact on the livestock industry. Since MAP is not inactivated during pasteurization, human population is continuously at the risk of getting exposed to MAP infection through consumption of dairy products. There is need for new candidate genes and/or proteins for developing improved diagnostic assays for the diagnosis of MAP infection and for the control of disease. Increasing evidences showed that expression of mce genes is important for the virulence of MAP. Whole-genome DNA microarray representing MAP revealed that there are 14 large sequence polymorphisms with LSPP12 being the most widely conserved MAP-specific region that included a cluster of six homologs of mce-family involved in lipid metabolism. On the other hand, LSP11 comprising part of mce2 operon was absent in MAP isolates. This review summarizes the advancement of research on mce genes of Mycobacteria with special reference to the MAP infection. |
topic |
mce operon mycobacteria protein diagnosis map mtb johne’s disease |
url |
http://dx.doi.org/10.1080/01652176.2019.1641764 |
work_keys_str_mv |
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