Production of recombinant flagellin to develop ELISA-based detection of Salmonella Enteritidis

• Main Authors: Seyed Ali Mirhosseini, Abbas Ali Imani Fooladi, Jafar Amani, Hamid Sedighian
• Format: Article
• Language: English
• Published: Sociedade Brasileira de Microbiologia
• Series: Brazilian Journal of Microbiology
• Subjects: Salmonella Enteritidis; Flagellin C; Indirect ELISA; Bacterial detection; Food contaminated
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400774&lng=en&tlng=en

ABSTRACT Food-borne diseases, caused by the pathogenic bacteria, are highly prevalent in the world. Salmonella is one of the most important bacterial genera responsible for this. Salmonella Enteritidis (SE) is one of the non-typhoid Salmonellae that can be transmitted to human from poultry products, water, and contaminated food. In recent years, new and rapid detection methods such as enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) have been developed. In this study, recombinant FliC (rFliC) was produced to be used as an antigen. The immunization was conducted in mice with the purified recombinant FliC (rFliC). The mice were subcutaneously immunized with rFliC and elicited significant rFliC specific serum IgG antibodies. An indirect ELISA system was established for the detection of Salmonella Enteritidis. Our results confirmed that the recombinant flagellin can be one of the excellent indicators for the detection of Salmonella Enteritidis.