Isolation of Chromatin from Dysfunctional Telomeres Reveals an Important Role for Ring1b in NHEJ-Mediated Chromosome Fusions
When telomeres become critically short, DNA damage response factors are recruited at chromosome ends, initiating a cellular response to DNA damage. We performed proteomic isolation of chromatin fragments (PICh) in order to define changes in chromatin composition that occur upon onset of acute telome...
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doaj-57f82cbc31b8482197a216bc3a0982c72020-11-24T20:58:23ZengElsevierCell Reports2211-12472014-05-01741320133210.1016/j.celrep.2014.04.002Isolation of Chromatin from Dysfunctional Telomeres Reveals an Important Role for Ring1b in NHEJ-Mediated Chromosome FusionsCristina Bartocci0Jolene K. Diedrich1Iliana Ouzounov2Julia Li3Andrea Piunti4Diego Pasini5John R. Yates III6Eros Lazzerini Denchi7Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USADepartment of Chemical Physiology, The Scripps Research Institute, La Jolla, CA 92037, USADepartment of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USADepartment of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USADepartment of Experimental Oncology, European Institute of Oncology, 20146 Milan, ItalyDepartment of Experimental Oncology, European Institute of Oncology, 20146 Milan, ItalyDepartment of Chemical Physiology, The Scripps Research Institute, La Jolla, CA 92037, USADepartment of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USAWhen telomeres become critically short, DNA damage response factors are recruited at chromosome ends, initiating a cellular response to DNA damage. We performed proteomic isolation of chromatin fragments (PICh) in order to define changes in chromatin composition that occur upon onset of acute telomere dysfunction triggered by depletion of the telomere-associated factor TRF2. This unbiased purification of telomere-associated proteins in functional or dysfunctional conditions revealed the dynamic changes in chromatin composition that take place at telomeres upon DNA damage induction. On the basis of our results, we describe a critical role for the polycomb group protein Ring1b in nonhomologous end-joining (NHEJ)-mediated end-to-end chromosome fusions. We show that cells with reduced levels of Ring1b have a reduced ability to repair uncapped telomeric chromatin. Our data represent an unbiased isolation of chromatin undergoing DNA damage and are a valuable resource to map the changes in chromatin composition in response to DNA damage activation.http://www.sciencedirect.com/science/article/pii/S2211124714002897 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Cristina Bartocci Jolene K. Diedrich Iliana Ouzounov Julia Li Andrea Piunti Diego Pasini John R. Yates III Eros Lazzerini Denchi |
spellingShingle |
Cristina Bartocci Jolene K. Diedrich Iliana Ouzounov Julia Li Andrea Piunti Diego Pasini John R. Yates III Eros Lazzerini Denchi Isolation of Chromatin from Dysfunctional Telomeres Reveals an Important Role for Ring1b in NHEJ-Mediated Chromosome Fusions Cell Reports |
author_facet |
Cristina Bartocci Jolene K. Diedrich Iliana Ouzounov Julia Li Andrea Piunti Diego Pasini John R. Yates III Eros Lazzerini Denchi |
author_sort |
Cristina Bartocci |
title |
Isolation of Chromatin from Dysfunctional Telomeres Reveals an Important Role for Ring1b in NHEJ-Mediated Chromosome Fusions |
title_short |
Isolation of Chromatin from Dysfunctional Telomeres Reveals an Important Role for Ring1b in NHEJ-Mediated Chromosome Fusions |
title_full |
Isolation of Chromatin from Dysfunctional Telomeres Reveals an Important Role for Ring1b in NHEJ-Mediated Chromosome Fusions |
title_fullStr |
Isolation of Chromatin from Dysfunctional Telomeres Reveals an Important Role for Ring1b in NHEJ-Mediated Chromosome Fusions |
title_full_unstemmed |
Isolation of Chromatin from Dysfunctional Telomeres Reveals an Important Role for Ring1b in NHEJ-Mediated Chromosome Fusions |
title_sort |
isolation of chromatin from dysfunctional telomeres reveals an important role for ring1b in nhej-mediated chromosome fusions |
publisher |
Elsevier |
series |
Cell Reports |
issn |
2211-1247 |
publishDate |
2014-05-01 |
description |
When telomeres become critically short, DNA damage response factors are recruited at chromosome ends, initiating a cellular response to DNA damage. We performed proteomic isolation of chromatin fragments (PICh) in order to define changes in chromatin composition that occur upon onset of acute telomere dysfunction triggered by depletion of the telomere-associated factor TRF2. This unbiased purification of telomere-associated proteins in functional or dysfunctional conditions revealed the dynamic changes in chromatin composition that take place at telomeres upon DNA damage induction. On the basis of our results, we describe a critical role for the polycomb group protein Ring1b in nonhomologous end-joining (NHEJ)-mediated end-to-end chromosome fusions. We show that cells with reduced levels of Ring1b have a reduced ability to repair uncapped telomeric chromatin. Our data represent an unbiased isolation of chromatin undergoing DNA damage and are a valuable resource to map the changes in chromatin composition in response to DNA damage activation. |
url |
http://www.sciencedirect.com/science/article/pii/S2211124714002897 |
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