Low Frequency Electrical Stimulation Attenuated The Epileptiform Activity-Induced Changes in Action Potential Features in Hippocampal CA1 Pyramidal Neurons
Objective Electrical low frequency stimulation (LFS) is a new therapeutic method that moderates hyperexcitability during epileptic states. Seizure occurrence is accompanied by some changes in action potential (AP) features. In this study, we investigated the inhibitory action of LFS on epileptiform...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Royan Institute (ACECR), Tehran
2018-06-01
|
Series: | Cell Journal |
Subjects: | |
Online Access: | http://celljournal.org/journal/article/21087/download |
id |
doaj-580067c51f6f4716b99d78c725f04bb1 |
---|---|
record_format |
Article |
spelling |
doaj-580067c51f6f4716b99d78c725f04bb12020-11-25T01:37:47ZengRoyan Institute (ACECR), TehranCell Journal2228-58062228-58142018-06-0120335536010.22074/cellj.2018.5443Low Frequency Electrical Stimulation Attenuated The Epileptiform Activity-Induced Changes in Action Potential Features in Hippocampal CA1 Pyramidal NeuronsZahra Ghasemi 0Nima Naderi1Amir Shojaei2Nooshin Ahmadirad3Mohammad Reza Raoufy4Javad Mirnajafi-Zadeh5Department of Physiology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranDepartment of Pharmacology and Toxicology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Physiology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranDepartment of Physiology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranDepartment of Physiology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranDepartment of Physiology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranObjective Electrical low frequency stimulation (LFS) is a new therapeutic method that moderates hyperexcitability during epileptic states. Seizure occurrence is accompanied by some changes in action potential (AP) features. In this study, we investigated the inhibitory action of LFS on epileptiform activity (EA) induced-changes in AP features in hippocampal CA1 pyramidal neurons. Materials and Methods In this experimental study, we induced EA in hippocampal slices by increasing the extracellular potassium (K+) concentration to 12 mM. LFS (1 Hz) was applied to the Schaffer collaterals at different pulse numbers (600 and 900) at the beginning of the EA. Changes in AP features recorded by whole-cell patch clamp recording were compared using phase plot analysis. Results Induction of EA depolarized membrane potential, decreased peak amplitude, as well as the maximum rise and decay slopes of APs. Administration of 1 Hz LFS at the beginning of EA prevented the above mentioned changes in AP features. This suppressive effect of LFS depended on the LFS pulse number, such that application of 900 pulses of LFS had a stronger recovery effect on AP features that changed during EA compared to 600 pulses of LFS. The constructed phase plots of APs revealed that LFS at 900 pulses significantly decreased the changes in resting membrane potential (RMP), peak amplitude, and maximum rise and decay slopes that appeared during EA. Conclusion Increasing the numbers of LFS pulses can magnify its inhibitory effects on EA-induced changes in AP features.http://celljournal.org/journal/article/21087/downloadAction PotentialBrain StimulationHippocampusRat |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zahra Ghasemi Nima Naderi Amir Shojaei Nooshin Ahmadirad Mohammad Reza Raoufy Javad Mirnajafi-Zadeh |
spellingShingle |
Zahra Ghasemi Nima Naderi Amir Shojaei Nooshin Ahmadirad Mohammad Reza Raoufy Javad Mirnajafi-Zadeh Low Frequency Electrical Stimulation Attenuated The Epileptiform Activity-Induced Changes in Action Potential Features in Hippocampal CA1 Pyramidal Neurons Cell Journal Action Potential Brain Stimulation Hippocampus Rat |
author_facet |
Zahra Ghasemi Nima Naderi Amir Shojaei Nooshin Ahmadirad Mohammad Reza Raoufy Javad Mirnajafi-Zadeh |
author_sort |
Zahra Ghasemi |
title |
Low Frequency Electrical Stimulation Attenuated The Epileptiform Activity-Induced Changes in Action Potential Features in Hippocampal CA1 Pyramidal Neurons |
title_short |
Low Frequency Electrical Stimulation Attenuated The Epileptiform Activity-Induced Changes in Action Potential Features in Hippocampal CA1 Pyramidal Neurons |
title_full |
Low Frequency Electrical Stimulation Attenuated The Epileptiform Activity-Induced Changes in Action Potential Features in Hippocampal CA1 Pyramidal Neurons |
title_fullStr |
Low Frequency Electrical Stimulation Attenuated The Epileptiform Activity-Induced Changes in Action Potential Features in Hippocampal CA1 Pyramidal Neurons |
title_full_unstemmed |
Low Frequency Electrical Stimulation Attenuated The Epileptiform Activity-Induced Changes in Action Potential Features in Hippocampal CA1 Pyramidal Neurons |
title_sort |
low frequency electrical stimulation attenuated the epileptiform activity-induced changes in action potential features in hippocampal ca1 pyramidal neurons |
publisher |
Royan Institute (ACECR), Tehran |
series |
Cell Journal |
issn |
2228-5806 2228-5814 |
publishDate |
2018-06-01 |
description |
Objective
Electrical low frequency stimulation (LFS) is a new therapeutic method that moderates hyperexcitability during epileptic states. Seizure occurrence is accompanied by some changes in action potential (AP) features. In this study, we investigated the inhibitory action of LFS on epileptiform activity (EA) induced-changes in AP features in hippocampal CA1 pyramidal neurons.
Materials and Methods
In this experimental study, we induced EA in hippocampal slices by increasing the extracellular potassium (K+) concentration to 12 mM. LFS (1 Hz) was applied to the Schaffer collaterals at different pulse numbers (600 and 900) at the beginning of the EA. Changes in AP features recorded by whole-cell patch clamp recording were compared using phase plot analysis.
Results
Induction of EA depolarized membrane potential, decreased peak amplitude, as well as the maximum rise and decay slopes of APs. Administration of 1 Hz LFS at the beginning of EA prevented the above mentioned changes in AP features. This suppressive effect of LFS depended on the LFS pulse number, such that application of 900 pulses of LFS had a stronger recovery effect on AP features that changed during EA compared to 600 pulses of LFS. The constructed phase plots of APs revealed that LFS at 900 pulses significantly decreased the changes in resting membrane potential (RMP), peak amplitude, and maximum rise and decay slopes that appeared during EA.
Conclusion
Increasing the numbers of LFS pulses can magnify its inhibitory effects on EA-induced changes in AP features. |
topic |
Action Potential Brain Stimulation Hippocampus Rat |
url |
http://celljournal.org/journal/article/21087/download |
work_keys_str_mv |
AT zahraghasemi lowfrequencyelectricalstimulationattenuatedtheepileptiformactivityinducedchangesinactionpotentialfeaturesinhippocampalca1pyramidalneurons AT nimanaderi lowfrequencyelectricalstimulationattenuatedtheepileptiformactivityinducedchangesinactionpotentialfeaturesinhippocampalca1pyramidalneurons AT amirshojaei lowfrequencyelectricalstimulationattenuatedtheepileptiformactivityinducedchangesinactionpotentialfeaturesinhippocampalca1pyramidalneurons AT nooshinahmadirad lowfrequencyelectricalstimulationattenuatedtheepileptiformactivityinducedchangesinactionpotentialfeaturesinhippocampalca1pyramidalneurons AT mohammadrezaraoufy lowfrequencyelectricalstimulationattenuatedtheepileptiformactivityinducedchangesinactionpotentialfeaturesinhippocampalca1pyramidalneurons AT javadmirnajafizadeh lowfrequencyelectricalstimulationattenuatedtheepileptiformactivityinducedchangesinactionpotentialfeaturesinhippocampalca1pyramidalneurons |
_version_ |
1725057444486316032 |