Heat Shock Protein Augmentation of Root Hot Water Extract on Adipogenic Differentiation in Murine 3T3-L1 Preadipocytes
There is a high association of heat shock on the alteration of energy and lipid metabolism. The alterations associated with thermal stress are composed of gene expression changes and adaptation through biochemical responses. Previous study showed that Angelica gigas Nakai (AGN) root extract promoted...
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Asian-Australasian Association of Animal Production Societies
2016-03-01
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doaj-580a8f1603ea486999c426783eb28cb52020-11-24T21:54:40ZengAsian-Australasian Association of Animal Production SocietiesAsian-Australasian Journal of Animal Sciences1011-23671976-55172016-03-0129341942710.5713/ajas.15.067723433Heat Shock Protein Augmentation of Root Hot Water Extract on Adipogenic Differentiation in Murine 3T3-L1 PreadipocytesWenchie Marie L. Lumbera0Joseph dela Cruz1Seung-Hak Yang2Seong Gu Hwang College of Veterinary Medicine, University of the Philippines Los Banos, 4030, .Philippines College of Veterinary Medicine, University of the Philippines Los Banos, 4030, .Philippines National Institute of Animal Science, Rural Development Administration, Wanju 55365, .KoreaThere is a high association of heat shock on the alteration of energy and lipid metabolism. The alterations associated with thermal stress are composed of gene expression changes and adaptation through biochemical responses. Previous study showed that Angelica gigas Nakai (AGN) root extract promoted adipogenic differentiation in murine 3T3-L1 preadipocytes under the normal temperature condition. However, its effect in heat shocked 3T3-L1 cells has not been established. In this study, we investigated the effect of AGN root hot water extract in the adipogenic differentiation of murine 3T3-L1 preadipocytes following heat shock and its possible mechanism of action. Thermal stress procedure was executed within the same stage of preadipocyte confluence (G0) through incubation at 42°C for one hour and then allowed to recover at normal incubation temperature of 37°C for another hour before AGN treatment for both cell viability assay and Oil Red O. Cell viability assay showed that AGN was able to dose dependently (0 to 400 μg/mL) increase cell proliferation under normal incubation temperature and also was able to prevent cytotoxicity due to heat shock accompanied by cell proliferation. Confluent preadipocytes were subjected into heat shock procedure, recovery and then AGN treatment prior to stimulation with the differentiation solution. Heat shocked preadipocytes exhibited reduced differentiation as supported by decreased amount of lipid accumulation in Oil Red O staining and triglyceride measurement. However, those heat shocked preadipocytes that then were given AGN extract showed a dose dependent increase in lipid accumulation as shown by both evaluation procedures. In line with these results, real-time polymerase chain reaction (RT-PCR) and Western blot analysis showed that AGN increased adipogenic differentiation by upregulating heat shock protection related genes and proteins together with the adipogenic markers. These findings imply the potential of AGN in heat shock amelioration among 3T3-L1 preadipocytes through heat shock factor and proteins augmentation and enhanced adipogenic marker expression.http://www.ajas.info/upload/pdf/ajas-29-3-419.pdfHeat ShockHeat Shock Protein3T3-L1Adipogenesis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wenchie Marie L. Lumbera Joseph dela Cruz Seung-Hak Yang Seong Gu Hwang |
spellingShingle |
Wenchie Marie L. Lumbera Joseph dela Cruz Seung-Hak Yang Seong Gu Hwang Heat Shock Protein Augmentation of Root Hot Water Extract on Adipogenic Differentiation in Murine 3T3-L1 Preadipocytes Asian-Australasian Journal of Animal Sciences Heat Shock Heat Shock Protein 3T3-L1 Adipogenesis |
author_facet |
Wenchie Marie L. Lumbera Joseph dela Cruz Seung-Hak Yang Seong Gu Hwang |
author_sort |
Wenchie Marie L. Lumbera |
title |
Heat Shock Protein Augmentation of Root Hot Water Extract on Adipogenic Differentiation in Murine 3T3-L1 Preadipocytes |
title_short |
Heat Shock Protein Augmentation of Root Hot Water Extract on Adipogenic Differentiation in Murine 3T3-L1 Preadipocytes |
title_full |
Heat Shock Protein Augmentation of Root Hot Water Extract on Adipogenic Differentiation in Murine 3T3-L1 Preadipocytes |
title_fullStr |
Heat Shock Protein Augmentation of Root Hot Water Extract on Adipogenic Differentiation in Murine 3T3-L1 Preadipocytes |
title_full_unstemmed |
Heat Shock Protein Augmentation of Root Hot Water Extract on Adipogenic Differentiation in Murine 3T3-L1 Preadipocytes |
title_sort |
heat shock protein augmentation of root hot water extract on adipogenic differentiation in murine 3t3-l1 preadipocytes |
publisher |
Asian-Australasian Association of Animal Production Societies |
series |
Asian-Australasian Journal of Animal Sciences |
issn |
1011-2367 1976-5517 |
publishDate |
2016-03-01 |
description |
There is a high association of heat shock on the alteration of energy and lipid metabolism. The alterations associated with thermal stress are composed of gene expression changes and adaptation through biochemical responses. Previous study showed that Angelica gigas Nakai (AGN) root extract promoted adipogenic differentiation in murine 3T3-L1 preadipocytes under the normal temperature condition. However, its effect in heat shocked 3T3-L1 cells has not been established. In this study, we investigated the effect of AGN root hot water extract in the adipogenic differentiation of murine 3T3-L1 preadipocytes following heat shock and its possible mechanism of action. Thermal stress procedure was executed within the same stage of preadipocyte confluence (G0) through incubation at 42°C for one hour and then allowed to recover at normal incubation temperature of 37°C for another hour before AGN treatment for both cell viability assay and Oil Red O. Cell viability assay showed that AGN was able to dose dependently (0 to 400 μg/mL) increase cell proliferation under normal incubation temperature and also was able to prevent cytotoxicity due to heat shock accompanied by cell proliferation. Confluent preadipocytes were subjected into heat shock procedure, recovery and then AGN treatment prior to stimulation with the differentiation solution. Heat shocked preadipocytes exhibited reduced differentiation as supported by decreased amount of lipid accumulation in Oil Red O staining and triglyceride measurement. However, those heat shocked preadipocytes that then were given AGN extract showed a dose dependent increase in lipid accumulation as shown by both evaluation procedures. In line with these results, real-time polymerase chain reaction (RT-PCR) and Western blot analysis showed that AGN increased adipogenic differentiation by upregulating heat shock protection related genes and proteins together with the adipogenic markers. These findings imply the potential of AGN in heat shock amelioration among 3T3-L1 preadipocytes through heat shock factor and proteins augmentation and enhanced adipogenic marker expression. |
topic |
Heat Shock Heat Shock Protein 3T3-L1 Adipogenesis |
url |
http://www.ajas.info/upload/pdf/ajas-29-3-419.pdf |
work_keys_str_mv |
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