The Potential Mechanism of ZFX Involvement in the Cell Growth
Background:The zinc-finger X linked (ZFX) gene encodes a transcription factor that acts as a regulator of self-renewal of stem cells. Due to the role of ZFX in cell growth, understanding ZFX protein-protein interactions helps to clarify its proper biological functions in signaling pathways. The aim...
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Mazandaran University of Medical Sciences and Health Services
2016-04-01
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doaj-580b3c61294d46af93961ead60036dfe2020-11-25T03:29:33ZengMazandaran University of Medical Sciences and Health ServicesResearch in Molecular Medicine2322-13482322-133X2016-04-01422429The Potential Mechanism of ZFX Involvement in the Cell GrowthMahboube Ganji arjenaki0Parvaneh Nikpour1Mojtaba Emadi-bygi2 Department of Genetics, Faculty of Basic Sciences,University ofShahrekord, Shahrekord, Iran Research Institute of Biotechnology, University of Shahrekord, Shahrekord, Iran Applied Physiology Research Center, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran Background:The zinc-finger X linked (ZFX) gene encodes a transcription factor that acts as a regulator of self-renewal of stem cells. Due to the role of ZFX in cell growth, understanding ZFX protein-protein interactions helps to clarify its proper biological functions in signaling pathways. The aim of this study is to define ZFX protein-protein interactions and the role of ZFX in cell growth. Materials and Methods: The PIPs output includes three interacting proteins with ZFX: eukaryotic translation initiation factor 3 subunit I(EIF3I), eukaryotic translation initiation factor 3 subunit G(EIF3G) and protein nuclear pore and COPII coat complex component homolog isoform 3 (SEC13L1). Results: As a cargo and transmembrane protein interacting with Sec13,eIF3I and eIF3G, ZFX mediates cargo sorting in COPII vesicles at ER exit sites. While traveling to cis-Golgi, eIF3I is phosphorylated by the mechanistic target of rapamycin (mTOR). Proteins transport by COPI vesicles to the nucleusouter site layer containing SEC13 via the contribution of microtubules. EIF3G and eIF3I interact with coatomer protein complex subunit beta 2 (COPB2) that helps to enclose ZFX in COPI vesicle. ZFX and eIF3G enter nucleolus where activation of transcription from pre rDNA genes occurs. Conclusion:We proposed a model in which ZFX is involved in cell growth by promoting the transcription of rDNA genes.http://rmm.mazums.ac.ir/browse.php?a_code=A-10-795-2&slc_lang=en&sid=1ZFX Cell growth Cancer stem cells rDNA genes Protein-protein interactions |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mahboube Ganji arjenaki Parvaneh Nikpour Mojtaba Emadi-bygi |
spellingShingle |
Mahboube Ganji arjenaki Parvaneh Nikpour Mojtaba Emadi-bygi The Potential Mechanism of ZFX Involvement in the Cell Growth Research in Molecular Medicine ZFX Cell growth Cancer stem cells rDNA genes Protein-protein interactions |
author_facet |
Mahboube Ganji arjenaki Parvaneh Nikpour Mojtaba Emadi-bygi |
author_sort |
Mahboube Ganji arjenaki |
title |
The Potential Mechanism of ZFX Involvement in the Cell Growth |
title_short |
The Potential Mechanism of ZFX Involvement in the Cell Growth |
title_full |
The Potential Mechanism of ZFX Involvement in the Cell Growth |
title_fullStr |
The Potential Mechanism of ZFX Involvement in the Cell Growth |
title_full_unstemmed |
The Potential Mechanism of ZFX Involvement in the Cell Growth |
title_sort |
potential mechanism of zfx involvement in the cell growth |
publisher |
Mazandaran University of Medical Sciences and Health Services |
series |
Research in Molecular Medicine |
issn |
2322-1348 2322-133X |
publishDate |
2016-04-01 |
description |
Background:The zinc-finger X linked (ZFX) gene encodes a transcription factor that acts as a regulator of self-renewal of stem cells. Due to the role of ZFX in cell growth, understanding ZFX protein-protein interactions helps to clarify its proper biological functions in signaling pathways. The aim of this study is to define ZFX protein-protein interactions and the role of ZFX in cell growth.
Materials and Methods: The PIPs output includes three interacting proteins with ZFX: eukaryotic translation initiation factor 3 subunit I(EIF3I), eukaryotic translation initiation factor 3 subunit G(EIF3G) and protein nuclear pore and COPII coat complex component homolog isoform 3 (SEC13L1).
Results: As a cargo and transmembrane protein interacting with Sec13,eIF3I and eIF3G, ZFX mediates cargo sorting in COPII vesicles at ER exit sites. While traveling to cis-Golgi, eIF3I is phosphorylated by the mechanistic target of rapamycin (mTOR). Proteins transport by COPI vesicles to the nucleusouter site layer containing SEC13 via the contribution of microtubules. EIF3G and eIF3I interact with coatomer protein complex subunit beta 2 (COPB2) that helps to enclose ZFX in COPI vesicle. ZFX and eIF3G enter nucleolus where activation of transcription from pre rDNA genes occurs.
Conclusion:We proposed a model in which ZFX is involved in cell growth by promoting the transcription of rDNA genes. |
topic |
ZFX Cell growth Cancer stem cells rDNA genes Protein-protein interactions |
url |
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-795-2&slc_lang=en&sid=1 |
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