Xyloglucan Oligosaccharides Hydrolysis by Exo-Acting Glycoside Hydrolases from Hyperthermophilic Microorganism <i>Saccharolobus solfataricus</i>
In the field of biocatalysis and the development of a bio-based economy, hemicellulases have attracted great interest for various applications in industrial processes. However, the study of the catalytic activity of the lignocellulose-degrading enzymes needs to be improved to achieve the efficient h...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2021-03-01
|
Series: | International Journal of Molecular Sciences |
Subjects: | |
Online Access: | https://www.mdpi.com/1422-0067/22/7/3325 |
id |
doaj-581f63fc9b5a447c9ce4f1b37e7d6ba6 |
---|---|
record_format |
Article |
spelling |
doaj-581f63fc9b5a447c9ce4f1b37e7d6ba62021-03-25T00:04:22ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-03-01223325332510.3390/ijms22073325Xyloglucan Oligosaccharides Hydrolysis by Exo-Acting Glycoside Hydrolases from Hyperthermophilic Microorganism <i>Saccharolobus solfataricus</i>Nicola Curci0Andrea Strazzulli1Roberta Iacono2Federica De Lise3Luisa Maurelli4Mauro Di Fenza5Beatrice Cobucci-Ponzano6Marco Moracci7Department of Biology, University of Naples “Federico II”, Complesso Universitario di Monte S. Angelo, 80126 Naples, ItalyDepartment of Biology, University of Naples “Federico II”, Complesso Universitario di Monte S. Angelo, 80126 Naples, ItalyDepartment of Biology, University of Naples “Federico II”, Complesso Universitario di Monte S. Angelo, 80126 Naples, ItalyInstitute of Biosciences and BioResources—National Research Council of Italy, 80131 Naples, ItalyInstitute of Biosciences and BioResources—National Research Council of Italy, 80131 Naples, ItalyInstitute of Biosciences and BioResources—National Research Council of Italy, 80131 Naples, ItalyInstitute of Biosciences and BioResources—National Research Council of Italy, 80131 Naples, ItalyDepartment of Biology, University of Naples “Federico II”, Complesso Universitario di Monte S. Angelo, 80126 Naples, ItalyIn the field of biocatalysis and the development of a bio-based economy, hemicellulases have attracted great interest for various applications in industrial processes. However, the study of the catalytic activity of the lignocellulose-degrading enzymes needs to be improved to achieve the efficient hydrolysis of plant biomasses. In this framework, hemicellulases from hyperthermophilic archaea show interesting features as biocatalysts and provide many advantages in industrial applications thanks to their stability in the harsh conditions encountered during the pretreatment process. However, the hemicellulases from archaea are less studied compared to their bacterial counterpart, and the activity of most of them has been barely tested on natural substrates. Here, we investigated the hydrolysis of xyloglucan oligosaccharides from two different plants by using, both synergistically and individually, three glycoside hydrolases from <i>Saccharolobus solfataricus</i>: a GH1 β-gluco-/β-galactosidase, a α-fucosidase belonging to GH29, and a α-xylosidase from GH31. The results showed that the three enzymes were able to release monosaccharides from xyloglucan oligosaccharides after incubation at 65 °C. The concerted actions of β-gluco-/β-galactosidase and the α-xylosidase on both xyloglucan oligosaccharides have been observed, while the α-fucosidase was capable of releasing all α-linked fucose units from xyloglucan from apple pomace, representing the first GH29 enzyme belonging to subfamily A that is active on xyloglucan.https://www.mdpi.com/1422-0067/22/7/3325glycoside hydrolases<i>Saccharolobus solfataricus</i>xyloglucanpolysaccharide degradationarchaea |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Nicola Curci Andrea Strazzulli Roberta Iacono Federica De Lise Luisa Maurelli Mauro Di Fenza Beatrice Cobucci-Ponzano Marco Moracci |
spellingShingle |
Nicola Curci Andrea Strazzulli Roberta Iacono Federica De Lise Luisa Maurelli Mauro Di Fenza Beatrice Cobucci-Ponzano Marco Moracci Xyloglucan Oligosaccharides Hydrolysis by Exo-Acting Glycoside Hydrolases from Hyperthermophilic Microorganism <i>Saccharolobus solfataricus</i> International Journal of Molecular Sciences glycoside hydrolases <i>Saccharolobus solfataricus</i> xyloglucan polysaccharide degradation archaea |
author_facet |
Nicola Curci Andrea Strazzulli Roberta Iacono Federica De Lise Luisa Maurelli Mauro Di Fenza Beatrice Cobucci-Ponzano Marco Moracci |
author_sort |
Nicola Curci |
title |
Xyloglucan Oligosaccharides Hydrolysis by Exo-Acting Glycoside Hydrolases from Hyperthermophilic Microorganism <i>Saccharolobus solfataricus</i> |
title_short |
Xyloglucan Oligosaccharides Hydrolysis by Exo-Acting Glycoside Hydrolases from Hyperthermophilic Microorganism <i>Saccharolobus solfataricus</i> |
title_full |
Xyloglucan Oligosaccharides Hydrolysis by Exo-Acting Glycoside Hydrolases from Hyperthermophilic Microorganism <i>Saccharolobus solfataricus</i> |
title_fullStr |
Xyloglucan Oligosaccharides Hydrolysis by Exo-Acting Glycoside Hydrolases from Hyperthermophilic Microorganism <i>Saccharolobus solfataricus</i> |
title_full_unstemmed |
Xyloglucan Oligosaccharides Hydrolysis by Exo-Acting Glycoside Hydrolases from Hyperthermophilic Microorganism <i>Saccharolobus solfataricus</i> |
title_sort |
xyloglucan oligosaccharides hydrolysis by exo-acting glycoside hydrolases from hyperthermophilic microorganism <i>saccharolobus solfataricus</i> |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2021-03-01 |
description |
In the field of biocatalysis and the development of a bio-based economy, hemicellulases have attracted great interest for various applications in industrial processes. However, the study of the catalytic activity of the lignocellulose-degrading enzymes needs to be improved to achieve the efficient hydrolysis of plant biomasses. In this framework, hemicellulases from hyperthermophilic archaea show interesting features as biocatalysts and provide many advantages in industrial applications thanks to their stability in the harsh conditions encountered during the pretreatment process. However, the hemicellulases from archaea are less studied compared to their bacterial counterpart, and the activity of most of them has been barely tested on natural substrates. Here, we investigated the hydrolysis of xyloglucan oligosaccharides from two different plants by using, both synergistically and individually, three glycoside hydrolases from <i>Saccharolobus solfataricus</i>: a GH1 β-gluco-/β-galactosidase, a α-fucosidase belonging to GH29, and a α-xylosidase from GH31. The results showed that the three enzymes were able to release monosaccharides from xyloglucan oligosaccharides after incubation at 65 °C. The concerted actions of β-gluco-/β-galactosidase and the α-xylosidase on both xyloglucan oligosaccharides have been observed, while the α-fucosidase was capable of releasing all α-linked fucose units from xyloglucan from apple pomace, representing the first GH29 enzyme belonging to subfamily A that is active on xyloglucan. |
topic |
glycoside hydrolases <i>Saccharolobus solfataricus</i> xyloglucan polysaccharide degradation archaea |
url |
https://www.mdpi.com/1422-0067/22/7/3325 |
work_keys_str_mv |
AT nicolacurci xyloglucanoligosaccharideshydrolysisbyexoactingglycosidehydrolasesfromhyperthermophilicmicroorganismisaccharolobussolfataricusi AT andreastrazzulli xyloglucanoligosaccharideshydrolysisbyexoactingglycosidehydrolasesfromhyperthermophilicmicroorganismisaccharolobussolfataricusi AT robertaiacono xyloglucanoligosaccharideshydrolysisbyexoactingglycosidehydrolasesfromhyperthermophilicmicroorganismisaccharolobussolfataricusi AT federicadelise xyloglucanoligosaccharideshydrolysisbyexoactingglycosidehydrolasesfromhyperthermophilicmicroorganismisaccharolobussolfataricusi AT luisamaurelli xyloglucanoligosaccharideshydrolysisbyexoactingglycosidehydrolasesfromhyperthermophilicmicroorganismisaccharolobussolfataricusi AT maurodifenza xyloglucanoligosaccharideshydrolysisbyexoactingglycosidehydrolasesfromhyperthermophilicmicroorganismisaccharolobussolfataricusi AT beatricecobucciponzano xyloglucanoligosaccharideshydrolysisbyexoactingglycosidehydrolasesfromhyperthermophilicmicroorganismisaccharolobussolfataricusi AT marcomoracci xyloglucanoligosaccharideshydrolysisbyexoactingglycosidehydrolasesfromhyperthermophilicmicroorganismisaccharolobussolfataricusi |
_version_ |
1724204183884660736 |