Alzheimer’s Disease Mouse as a Model of Testis Degeneration
Pituitary adenylate cyclase activating polypeptide (PACAP) is a neuropeptide with protective functions in the central nervous system and various peripheral organs. PACAP has the highest expression level in the testes, among the peripheral organs, and has a positive regulative role in spermatogenesis...
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doaj-5825c1b5496c46b8906f1e065789d16a2020-11-25T03:55:42ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-08-01215726572610.3390/ijms21165726Alzheimer’s Disease Mouse as a Model of Testis DegenerationVince Szegeczki0Gabriella Horváth1Helga Perényi2Andrea Tamás3Zsolt Radák4Dóra Ábrahám5Róza Zákány6Dora Reglodi7Tamás Juhász8Department of Anatomy, Histology and Embryology, Faculty of Medicine, University of Debrecen, Nagyerdei krt. 98, H-4032 Debrecen, HungaryDepartment of Anatomy, PTE-MTA PACAP Research Team, University of Pécs Medical School, Szigeti út 12, H-7624 Pécs, HungaryDepartment of Anatomy, Histology and Embryology, Faculty of Medicine, University of Debrecen, Nagyerdei krt. 98, H-4032 Debrecen, HungaryDepartment of Anatomy, PTE-MTA PACAP Research Team, University of Pécs Medical School, Szigeti út 12, H-7624 Pécs, HungaryResearch Institute of Sport Science, University of Physical Education, Budapest, Alkotas út 44, H-1123 Budapest, HungaryResearch Institute of Sport Science, University of Physical Education, Budapest, Alkotas út 44, H-1123 Budapest, HungaryDepartment of Anatomy, Histology and Embryology, Faculty of Medicine, University of Debrecen, Nagyerdei krt. 98, H-4032 Debrecen, HungaryDepartment of Anatomy, PTE-MTA PACAP Research Team, University of Pécs Medical School, Szigeti út 12, H-7624 Pécs, HungaryDepartment of Anatomy, Histology and Embryology, Faculty of Medicine, University of Debrecen, Nagyerdei krt. 98, H-4032 Debrecen, HungaryPituitary adenylate cyclase activating polypeptide (PACAP) is a neuropeptide with protective functions in the central nervous system and various peripheral organs. PACAP has the highest expression level in the testes, among the peripheral organs, and has a positive regulative role in spermatogenesis and in sperm motility. In the present study, we explored testicular degenerative alterations in a mouse model of Alzheimer’s disease (AD) (B6C3-Tg(APPswe,PSEN1dE9)85Dbo/J) and demonstrated changes in PACAP-regulated signaling pathways. In addition, the effects of increased physical activity of AD (trained AD (TAD)) mice on testis were also followed. Reduced cell number and decreased thickness of basement membrane were detected in AD samples. These changes were compensated by physical activity. Expression of PACAP receptors and canonical signaling elements such as PKA, P-PKA, PP2A significantly decreased in AD mice, and altered Sox transcription factor expression was also detected. Via this signaling mechanism, physical activity compensated the negative effects of AD on the expression of type IV collagen. Our findings suggest that the testes of AD mice can be a good model of testis degeneration. Moreover, it can be an appropriate organ to follow the effects of various interventions such as physical activity on tissue regeneration and signaling alterations.https://www.mdpi.com/1422-0067/21/16/5726testis degenerationSox9Alzheimer’s diseasecollagen type IVphysical activity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Vince Szegeczki Gabriella Horváth Helga Perényi Andrea Tamás Zsolt Radák Dóra Ábrahám Róza Zákány Dora Reglodi Tamás Juhász |
spellingShingle |
Vince Szegeczki Gabriella Horváth Helga Perényi Andrea Tamás Zsolt Radák Dóra Ábrahám Róza Zákány Dora Reglodi Tamás Juhász Alzheimer’s Disease Mouse as a Model of Testis Degeneration International Journal of Molecular Sciences testis degeneration Sox9 Alzheimer’s disease collagen type IV physical activity |
author_facet |
Vince Szegeczki Gabriella Horváth Helga Perényi Andrea Tamás Zsolt Radák Dóra Ábrahám Róza Zákány Dora Reglodi Tamás Juhász |
author_sort |
Vince Szegeczki |
title |
Alzheimer’s Disease Mouse as a Model of Testis Degeneration |
title_short |
Alzheimer’s Disease Mouse as a Model of Testis Degeneration |
title_full |
Alzheimer’s Disease Mouse as a Model of Testis Degeneration |
title_fullStr |
Alzheimer’s Disease Mouse as a Model of Testis Degeneration |
title_full_unstemmed |
Alzheimer’s Disease Mouse as a Model of Testis Degeneration |
title_sort |
alzheimer’s disease mouse as a model of testis degeneration |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2020-08-01 |
description |
Pituitary adenylate cyclase activating polypeptide (PACAP) is a neuropeptide with protective functions in the central nervous system and various peripheral organs. PACAP has the highest expression level in the testes, among the peripheral organs, and has a positive regulative role in spermatogenesis and in sperm motility. In the present study, we explored testicular degenerative alterations in a mouse model of Alzheimer’s disease (AD) (B6C3-Tg(APPswe,PSEN1dE9)85Dbo/J) and demonstrated changes in PACAP-regulated signaling pathways. In addition, the effects of increased physical activity of AD (trained AD (TAD)) mice on testis were also followed. Reduced cell number and decreased thickness of basement membrane were detected in AD samples. These changes were compensated by physical activity. Expression of PACAP receptors and canonical signaling elements such as PKA, P-PKA, PP2A significantly decreased in AD mice, and altered Sox transcription factor expression was also detected. Via this signaling mechanism, physical activity compensated the negative effects of AD on the expression of type IV collagen. Our findings suggest that the testes of AD mice can be a good model of testis degeneration. Moreover, it can be an appropriate organ to follow the effects of various interventions such as physical activity on tissue regeneration and signaling alterations. |
topic |
testis degeneration Sox9 Alzheimer’s disease collagen type IV physical activity |
url |
https://www.mdpi.com/1422-0067/21/16/5726 |
work_keys_str_mv |
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