Identification of odorant-receptor interactions by global mapping of the human odorome.
The human olfactory system recognizes a broad spectrum of odorants using approximately 400 different olfactory receptors (hORs). Although significant improvements of heterologous expression systems used to study interactions between ORs and odorant molecules have been made, screening the olfactory r...
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doaj-582892b549754019885fef4dc8a619fc2020-11-24T21:50:43ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0194e9303710.1371/journal.pone.0093037Identification of odorant-receptor interactions by global mapping of the human odorome.Karine AudouzeAnne TromelinAnne Marie Le BonChristine BelloirRasmus Koefoed PetersenKarsten KristiansenSøren BrunakOlivier TaboureauThe human olfactory system recognizes a broad spectrum of odorants using approximately 400 different olfactory receptors (hORs). Although significant improvements of heterologous expression systems used to study interactions between ORs and odorant molecules have been made, screening the olfactory repertoire of hORs remains a tremendous challenge. We therefore developed a chemical systems level approach based on protein-protein association network to investigate novel hOR-odorant relationships. Using this new approach, we proposed and validated new bioactivities for odorant molecules and OR2W1, OR51E1 and OR5P3. As it remains largely unknown how human perception of odorants influence or prevent diseases, we also developed an odorant-protein matrix to explore global relationships between chemicals, biological targets and disease susceptibilities. We successfully experimentally demonstrated interactions between odorants and the cannabinoid receptor 1 (CB1) and the peroxisome proliferator-activated receptor gamma (PPARγ). Overall, these results illustrate the potential of integrative systems chemical biology to explore the impact of odorant molecules on human health, i.e. human odorome.http://europepmc.org/articles/PMC3973694?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Karine Audouze Anne Tromelin Anne Marie Le Bon Christine Belloir Rasmus Koefoed Petersen Karsten Kristiansen Søren Brunak Olivier Taboureau |
spellingShingle |
Karine Audouze Anne Tromelin Anne Marie Le Bon Christine Belloir Rasmus Koefoed Petersen Karsten Kristiansen Søren Brunak Olivier Taboureau Identification of odorant-receptor interactions by global mapping of the human odorome. PLoS ONE |
author_facet |
Karine Audouze Anne Tromelin Anne Marie Le Bon Christine Belloir Rasmus Koefoed Petersen Karsten Kristiansen Søren Brunak Olivier Taboureau |
author_sort |
Karine Audouze |
title |
Identification of odorant-receptor interactions by global mapping of the human odorome. |
title_short |
Identification of odorant-receptor interactions by global mapping of the human odorome. |
title_full |
Identification of odorant-receptor interactions by global mapping of the human odorome. |
title_fullStr |
Identification of odorant-receptor interactions by global mapping of the human odorome. |
title_full_unstemmed |
Identification of odorant-receptor interactions by global mapping of the human odorome. |
title_sort |
identification of odorant-receptor interactions by global mapping of the human odorome. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2014-01-01 |
description |
The human olfactory system recognizes a broad spectrum of odorants using approximately 400 different olfactory receptors (hORs). Although significant improvements of heterologous expression systems used to study interactions between ORs and odorant molecules have been made, screening the olfactory repertoire of hORs remains a tremendous challenge. We therefore developed a chemical systems level approach based on protein-protein association network to investigate novel hOR-odorant relationships. Using this new approach, we proposed and validated new bioactivities for odorant molecules and OR2W1, OR51E1 and OR5P3. As it remains largely unknown how human perception of odorants influence or prevent diseases, we also developed an odorant-protein matrix to explore global relationships between chemicals, biological targets and disease susceptibilities. We successfully experimentally demonstrated interactions between odorants and the cannabinoid receptor 1 (CB1) and the peroxisome proliferator-activated receptor gamma (PPARγ). Overall, these results illustrate the potential of integrative systems chemical biology to explore the impact of odorant molecules on human health, i.e. human odorome. |
url |
http://europepmc.org/articles/PMC3973694?pdf=render |
work_keys_str_mv |
AT karineaudouze identificationofodorantreceptorinteractionsbyglobalmappingofthehumanodorome AT annetromelin identificationofodorantreceptorinteractionsbyglobalmappingofthehumanodorome AT annemarielebon identificationofodorantreceptorinteractionsbyglobalmappingofthehumanodorome AT christinebelloir identificationofodorantreceptorinteractionsbyglobalmappingofthehumanodorome AT rasmuskoefoedpetersen identificationofodorantreceptorinteractionsbyglobalmappingofthehumanodorome AT karstenkristiansen identificationofodorantreceptorinteractionsbyglobalmappingofthehumanodorome AT sørenbrunak identificationofodorantreceptorinteractionsbyglobalmappingofthehumanodorome AT oliviertaboureau identificationofodorantreceptorinteractionsbyglobalmappingofthehumanodorome |
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