Dispersive Solid Phase Extraction Using Magnetic NanoparticlesPerformed in a Narrow-Bored Tube for Extraction of Atorvastatin,Losartan, and Valsartan in Plasma
Purpose: In this investigation, a new version of magnetic solid phase extraction (MSPE) performedin a narrow–bore tube has been proposed. In this study, hydrophobic octyl (C8) functionalizedFe3O4 magnetic nanoparticles (MNPs) stabilized by SiOH groups (Fe3O4@SiO2@C8) are used asmagnetic nano–sorbent...
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Tabriz University of Medical Sciences
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doaj-5829653abfb94333a8d92025fa6c213f2020-11-25T01:03:00ZengTabriz University of Medical Sciences Advanced Pharmaceutical Bulletin2228-58812251-73082019-02-019113814610.15171/apb.2019.017apb-19617Dispersive Solid Phase Extraction Using Magnetic NanoparticlesPerformed in a Narrow-Bored Tube for Extraction of Atorvastatin,Losartan, and Valsartan in PlasmaMir Ali Farajzadeh0Adeleh Yadeghari1Maryam Abbaspour2Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran.Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran.Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran.Purpose: In this investigation, a new version of magnetic solid phase extraction (MSPE) performedin a narrow–bore tube has been proposed. In this study, hydrophobic octyl (C8) functionalizedFe3O4 magnetic nanoparticles (MNPs) stabilized by SiOH groups (Fe3O4@SiO2@C8) are used asmagnetic nano–sorbents for the extraction of cardiovascular drugs from human plasma prior totheir determination by high performance liquid chromatography–photodiode array detection.Methods: After precipitation of the plasma proteins, the supernatant is diluted with deionizedwater and filled into the narrow–bore tube. Then mg–level of the sorbent is added into the tube.The sorbent is dispersed and moved down through the solution instead of passing the solutionfrom the cartridge. Using an external magnet, the collected nano–sorbents at the bottom of thetube are transferred on top of the solution and released to move down through the solution forthree times to increase the extraction efficiency.Results: The linearity of the assay was ranging from 0.4–500 mg mL-1. The limits of detectionand quantification of the method were obtained in the ranges of 0.05–0.07 and 0.16–0.24 mgL-1, respectively. The extraction recoveries were obtained in the range of 31–49%. Intra– andinter–day precisions were calculated and obtained in the ranges of 5–8 and 7%–9% for 0.5 mgL-1 of each analyte, and 5–6 and 6%–8% for 2 mg L-1 of each analyte, respectively.Conclusion: The proposed method was successfully used in determination of the studied drugsin patient’s plasmas.https://apb.tbzmed.ac.ir/PDF/apb-19617High–performance liquid chromatographyMagnetic solid phase extractionPlasmaAtorvastatinLosartanValsartan |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mir Ali Farajzadeh Adeleh Yadeghari Maryam Abbaspour |
spellingShingle |
Mir Ali Farajzadeh Adeleh Yadeghari Maryam Abbaspour Dispersive Solid Phase Extraction Using Magnetic NanoparticlesPerformed in a Narrow-Bored Tube for Extraction of Atorvastatin,Losartan, and Valsartan in Plasma Advanced Pharmaceutical Bulletin High–performance liquid chromatography Magnetic solid phase extraction Plasma Atorvastatin Losartan Valsartan |
author_facet |
Mir Ali Farajzadeh Adeleh Yadeghari Maryam Abbaspour |
author_sort |
Mir Ali Farajzadeh |
title |
Dispersive Solid Phase Extraction Using Magnetic NanoparticlesPerformed in a Narrow-Bored Tube for Extraction of Atorvastatin,Losartan, and Valsartan in Plasma |
title_short |
Dispersive Solid Phase Extraction Using Magnetic NanoparticlesPerformed in a Narrow-Bored Tube for Extraction of Atorvastatin,Losartan, and Valsartan in Plasma |
title_full |
Dispersive Solid Phase Extraction Using Magnetic NanoparticlesPerformed in a Narrow-Bored Tube for Extraction of Atorvastatin,Losartan, and Valsartan in Plasma |
title_fullStr |
Dispersive Solid Phase Extraction Using Magnetic NanoparticlesPerformed in a Narrow-Bored Tube for Extraction of Atorvastatin,Losartan, and Valsartan in Plasma |
title_full_unstemmed |
Dispersive Solid Phase Extraction Using Magnetic NanoparticlesPerformed in a Narrow-Bored Tube for Extraction of Atorvastatin,Losartan, and Valsartan in Plasma |
title_sort |
dispersive solid phase extraction using magnetic nanoparticlesperformed in a narrow-bored tube for extraction of atorvastatin,losartan, and valsartan in plasma |
publisher |
Tabriz University of Medical Sciences |
series |
Advanced Pharmaceutical Bulletin |
issn |
2228-5881 2251-7308 |
publishDate |
2019-02-01 |
description |
Purpose: In this investigation, a new version of magnetic solid phase extraction (MSPE) performedin a narrow–bore tube has been proposed. In this study, hydrophobic octyl (C8) functionalizedFe3O4 magnetic nanoparticles (MNPs) stabilized by SiOH groups (Fe3O4@SiO2@C8) are used asmagnetic nano–sorbents for the extraction of cardiovascular drugs from human plasma prior totheir determination by high performance liquid chromatography–photodiode array detection.Methods: After precipitation of the plasma proteins, the supernatant is diluted with deionizedwater and filled into the narrow–bore tube. Then mg–level of the sorbent is added into the tube.The sorbent is dispersed and moved down through the solution instead of passing the solutionfrom the cartridge. Using an external magnet, the collected nano–sorbents at the bottom of thetube are transferred on top of the solution and released to move down through the solution forthree times to increase the extraction efficiency.Results: The linearity of the assay was ranging from 0.4–500 mg mL-1. The limits of detectionand quantification of the method were obtained in the ranges of 0.05–0.07 and 0.16–0.24 mgL-1, respectively. The extraction recoveries were obtained in the range of 31–49%. Intra– andinter–day precisions were calculated and obtained in the ranges of 5–8 and 7%–9% for 0.5 mgL-1 of each analyte, and 5–6 and 6%–8% for 2 mg L-1 of each analyte, respectively.Conclusion: The proposed method was successfully used in determination of the studied drugsin patient’s plasmas. |
topic |
High–performance liquid chromatography Magnetic solid phase extraction Plasma Atorvastatin Losartan Valsartan |
url |
https://apb.tbzmed.ac.ir/PDF/apb-19617 |
work_keys_str_mv |
AT miralifarajzadeh dispersivesolidphaseextractionusingmagneticnanoparticlesperformedinanarrowboredtubeforextractionofatorvastatinlosartanandvalsartaninplasma AT adelehyadeghari dispersivesolidphaseextractionusingmagneticnanoparticlesperformedinanarrowboredtubeforextractionofatorvastatinlosartanandvalsartaninplasma AT maryamabbaspour dispersivesolidphaseextractionusingmagneticnanoparticlesperformedinanarrowboredtubeforextractionofatorvastatinlosartanandvalsartaninplasma |
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