Effect of Lactobacillus spp. on adhesion, invasion, and translocation of Campylobacter jejuni in chicken and pig small-intestinal epithelial cell lines
Abstract Background Campylobacter spp. are a major cause of bacterial food-borne diarrhoeal disease. This mainly arises through contamination of meat products during processing. For infection, Campylobacter spp. must adhere to epithelial cells of the mucus layer, survive conditions of the gastrointe...
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doaj-582af30fd01e49e8b50c15aa63644b582021-02-07T12:17:17ZengBMCBMC Veterinary Research1746-61482020-02-0116111410.1186/s12917-020-2238-5Effect of Lactobacillus spp. on adhesion, invasion, and translocation of Campylobacter jejuni in chicken and pig small-intestinal epithelial cell linesMaja Šikić Pogačar0Tomaž Langerholc1Dušanka Mičetić-Turk2Sonja Smole Možina3Anja Klančnik4Faculty of Medicine, University of MariborDepartment of Microbiology, Biochemistry, Molecular Biology and Biotechnology, Faculty of Agriculture and Life Science, University of MariborFaculty of Medicine, University of MariborDepartment of Food Science and Technology, Biotechnical Faculty, University of LjubljanaDepartment of Food Science and Technology, Biotechnical Faculty, University of LjubljanaAbstract Background Campylobacter spp. are a major cause of bacterial food-borne diarrhoeal disease. This mainly arises through contamination of meat products during processing. For infection, Campylobacter spp. must adhere to epithelial cells of the mucus layer, survive conditions of the gastrointestinal tract, and colonise the intestine of the host. Addition of probiotic bacteria might promote competitive adhesion to epithelial cells, consequently reducing Campylobacter jejuni colonisation. Effect of Lactobacillus spp. (PCS20, PCS22, PCS25, LGG, PCK9) on C. jejuni adhesion, invasion and translocation in pig (PSI cl.1) and chicken (B1OXI) small-intestine cell lines, as well as pig enterocytes (CLAB) was investigated. Results Overall, in competitive adhesion assays with PSI cl.1 and CLAB cell monolayers, the addition of Lactobacillus spp. reduced C. jejuni adherence to the cell surface, and negatively affected the C. jejuni invasion. Interestingly, Lactobacillus spp. significantly impaired C. jejuni adhesion in three-dimensional functional PSI cl.1 and B1OXI cell models. Also, C. jejuni did not translocate across PSI cl.1 and B1OXI cell monolayers when co-incubated with probiotics. Among selected probiotics, Lactobacillus rhamnosus LGG was the strain that reduced adhesion efficacy of C. jejuni most significantly under co-culture conditions. Conclusion The addition of Lactobacillus spp. to feed additives in livestock nutrition might be an effective novel strategy that targets Campylobacter adhesion to epithelial cells, and thus prevents colonisation, reduces the transmission, and finally lowers the incidence of human campylobacteriosis.https://doi.org/10.1186/s12917-020-2238-5Campylobacter jejuniLactobacillus spp.Chicken and pig cell lineAdhesionInvasionTranslocation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Maja Šikić Pogačar Tomaž Langerholc Dušanka Mičetić-Turk Sonja Smole Možina Anja Klančnik |
spellingShingle |
Maja Šikić Pogačar Tomaž Langerholc Dušanka Mičetić-Turk Sonja Smole Možina Anja Klančnik Effect of Lactobacillus spp. on adhesion, invasion, and translocation of Campylobacter jejuni in chicken and pig small-intestinal epithelial cell lines BMC Veterinary Research Campylobacter jejuni Lactobacillus spp. Chicken and pig cell line Adhesion Invasion Translocation |
author_facet |
Maja Šikić Pogačar Tomaž Langerholc Dušanka Mičetić-Turk Sonja Smole Možina Anja Klančnik |
author_sort |
Maja Šikić Pogačar |
title |
Effect of Lactobacillus spp. on adhesion, invasion, and translocation of Campylobacter jejuni in chicken and pig small-intestinal epithelial cell lines |
title_short |
Effect of Lactobacillus spp. on adhesion, invasion, and translocation of Campylobacter jejuni in chicken and pig small-intestinal epithelial cell lines |
title_full |
Effect of Lactobacillus spp. on adhesion, invasion, and translocation of Campylobacter jejuni in chicken and pig small-intestinal epithelial cell lines |
title_fullStr |
Effect of Lactobacillus spp. on adhesion, invasion, and translocation of Campylobacter jejuni in chicken and pig small-intestinal epithelial cell lines |
title_full_unstemmed |
Effect of Lactobacillus spp. on adhesion, invasion, and translocation of Campylobacter jejuni in chicken and pig small-intestinal epithelial cell lines |
title_sort |
effect of lactobacillus spp. on adhesion, invasion, and translocation of campylobacter jejuni in chicken and pig small-intestinal epithelial cell lines |
publisher |
BMC |
series |
BMC Veterinary Research |
issn |
1746-6148 |
publishDate |
2020-02-01 |
description |
Abstract Background Campylobacter spp. are a major cause of bacterial food-borne diarrhoeal disease. This mainly arises through contamination of meat products during processing. For infection, Campylobacter spp. must adhere to epithelial cells of the mucus layer, survive conditions of the gastrointestinal tract, and colonise the intestine of the host. Addition of probiotic bacteria might promote competitive adhesion to epithelial cells, consequently reducing Campylobacter jejuni colonisation. Effect of Lactobacillus spp. (PCS20, PCS22, PCS25, LGG, PCK9) on C. jejuni adhesion, invasion and translocation in pig (PSI cl.1) and chicken (B1OXI) small-intestine cell lines, as well as pig enterocytes (CLAB) was investigated. Results Overall, in competitive adhesion assays with PSI cl.1 and CLAB cell monolayers, the addition of Lactobacillus spp. reduced C. jejuni adherence to the cell surface, and negatively affected the C. jejuni invasion. Interestingly, Lactobacillus spp. significantly impaired C. jejuni adhesion in three-dimensional functional PSI cl.1 and B1OXI cell models. Also, C. jejuni did not translocate across PSI cl.1 and B1OXI cell monolayers when co-incubated with probiotics. Among selected probiotics, Lactobacillus rhamnosus LGG was the strain that reduced adhesion efficacy of C. jejuni most significantly under co-culture conditions. Conclusion The addition of Lactobacillus spp. to feed additives in livestock nutrition might be an effective novel strategy that targets Campylobacter adhesion to epithelial cells, and thus prevents colonisation, reduces the transmission, and finally lowers the incidence of human campylobacteriosis. |
topic |
Campylobacter jejuni Lactobacillus spp. Chicken and pig cell line Adhesion Invasion Translocation |
url |
https://doi.org/10.1186/s12917-020-2238-5 |
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