Extracellular vesicles provide a capsid-free vector for oncolytic adenoviral DNA delivery
Extracellular vesicles (EVs) have been showcased as auspicious candidates for delivering therapeutic cargo, including oncolytic viruses for cancer treatment. Delivery of oncolytic viruses in EVs could provide considerable advantages, hiding the viruses from the immune system and providing alternativ...
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2020-01-01
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Series: | Journal of Extracellular Vesicles |
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Online Access: | http://dx.doi.org/10.1080/20013078.2020.1747206 |
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doaj-582b3204dde84f99b2c2e8126bd263632020-11-25T02:20:41ZengTaylor & Francis GroupJournal of Extracellular Vesicles2001-30782020-01-019110.1080/20013078.2020.17472061747206Extracellular vesicles provide a capsid-free vector for oncolytic adenoviral DNA deliveryHeikki Saari0Tiia Turunen1Andres Lõhmus2Mikko Turunen3Matti Jalasvuori4Sarah J. Butcher5Seppo Ylä-Herttuala6Tapani Viitala7Vincenzo Cerullo8Pia R. M. Siljander9Marjo Yliperttula10University of HelsinkiUniversity of Eastern FinlandUniversity of HelsinkiUniversity of Eastern FinlandUniversity of JyväskyläUniversity of HelsinkiUniversity of Eastern FinlandUniversity of HelsinkiUniversity of HelsinkiUniversity of HelsinkiUniversity of HelsinkiExtracellular vesicles (EVs) have been showcased as auspicious candidates for delivering therapeutic cargo, including oncolytic viruses for cancer treatment. Delivery of oncolytic viruses in EVs could provide considerable advantages, hiding the viruses from the immune system and providing alternative entry pathways into cancer cells. Here we describe the formation and viral cargo of EVs secreted by cancer cells infected with an oncolytic adenovirus (IEVs, infected cell-derived EVs) as a function of time after infection. IEVs were secreted already before the lytic release of virions and their structure resembled normally secreted EVs, suggesting that they were not just apoptotic fragments of infected cells. IEVs were able to carry the viral genome and induce infection in other cancer cells. As such, the role of EVs in the life cycle of adenoviruses may be an important part of a successful infection and may also be harnessed for cancer- and gene therapy.http://dx.doi.org/10.1080/20013078.2020.1747206extracellular vesiclesadenoviruscancer therapydna delivery |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Heikki Saari Tiia Turunen Andres Lõhmus Mikko Turunen Matti Jalasvuori Sarah J. Butcher Seppo Ylä-Herttuala Tapani Viitala Vincenzo Cerullo Pia R. M. Siljander Marjo Yliperttula |
spellingShingle |
Heikki Saari Tiia Turunen Andres Lõhmus Mikko Turunen Matti Jalasvuori Sarah J. Butcher Seppo Ylä-Herttuala Tapani Viitala Vincenzo Cerullo Pia R. M. Siljander Marjo Yliperttula Extracellular vesicles provide a capsid-free vector for oncolytic adenoviral DNA delivery Journal of Extracellular Vesicles extracellular vesicles adenovirus cancer therapy dna delivery |
author_facet |
Heikki Saari Tiia Turunen Andres Lõhmus Mikko Turunen Matti Jalasvuori Sarah J. Butcher Seppo Ylä-Herttuala Tapani Viitala Vincenzo Cerullo Pia R. M. Siljander Marjo Yliperttula |
author_sort |
Heikki Saari |
title |
Extracellular vesicles provide a capsid-free vector for oncolytic adenoviral DNA delivery |
title_short |
Extracellular vesicles provide a capsid-free vector for oncolytic adenoviral DNA delivery |
title_full |
Extracellular vesicles provide a capsid-free vector for oncolytic adenoviral DNA delivery |
title_fullStr |
Extracellular vesicles provide a capsid-free vector for oncolytic adenoviral DNA delivery |
title_full_unstemmed |
Extracellular vesicles provide a capsid-free vector for oncolytic adenoviral DNA delivery |
title_sort |
extracellular vesicles provide a capsid-free vector for oncolytic adenoviral dna delivery |
publisher |
Taylor & Francis Group |
series |
Journal of Extracellular Vesicles |
issn |
2001-3078 |
publishDate |
2020-01-01 |
description |
Extracellular vesicles (EVs) have been showcased as auspicious candidates for delivering therapeutic cargo, including oncolytic viruses for cancer treatment. Delivery of oncolytic viruses in EVs could provide considerable advantages, hiding the viruses from the immune system and providing alternative entry pathways into cancer cells. Here we describe the formation and viral cargo of EVs secreted by cancer cells infected with an oncolytic adenovirus (IEVs, infected cell-derived EVs) as a function of time after infection. IEVs were secreted already before the lytic release of virions and their structure resembled normally secreted EVs, suggesting that they were not just apoptotic fragments of infected cells. IEVs were able to carry the viral genome and induce infection in other cancer cells. As such, the role of EVs in the life cycle of adenoviruses may be an important part of a successful infection and may also be harnessed for cancer- and gene therapy. |
topic |
extracellular vesicles adenovirus cancer therapy dna delivery |
url |
http://dx.doi.org/10.1080/20013078.2020.1747206 |
work_keys_str_mv |
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