Research of T-SOD and MDA in rabbit lens at the early period after vitrectomy combined with nonexpansile perfluoropropane gas

• Main Authors: Yan-Fang Yang, Song-Bo Jia, Luo-Sheng Tang
• Format: Article
• Language: English
• Published: Press of International Journal of Ophthalmology (IJO PRESS) 2015-11-01
• Series: Guoji Yanke Zazhi
• Subjects: vitrectomy; perfluoropropane gas; cataract; oxidative damage
• Online Access: http://ies.ijo.cn/cn_publish/2015/11/201511009.pdf
• ISSN: 1672-5123; 1672-5123

AIM:To study the relationship between oxidative damage and the early pathological changes in rabbit lens after vitrectomy combined with nonexpansile perfluoropropane gas, by detecting T-SOD activity and MDA content at different stages after the operation. <p>METHODS:Fourty five healthy new zealand rabbits were divided randomly to three groups:normal group, control group and experimental group. The control group was just performed vitrectomy with BSS; the experimental group was performed vitrectomy combined with nonexpansile perfluoropropane gas. The lens were made into tissue homogenates to detect T-SOD activity and MDA content by using spectrophotometry on the 1st,3th,8th,35th and 45th day after the operation. <p>RESULTS:T-SOD activity: that in normal group was the hightest, that in experimental group was the lowest, and there was significant difference(<i>P</i><0.01)among three groups in every periods after operation. MDA content: that in normal group was the lowest, that in experimental group was the hightest, and there was significant difference(<i>P</i><0.01)among three groups after the operation except the 1st day. <p>CONCLUSION:Oxidative damage mechanism may be involved in the early damge of rabbit lens after vitrectomy combined with nonexpansile perfluoropropane gas.