Isolation, characterization, cloning and bioinformatics analysis of a novel receptor from black cut worm (Agrotis ipsilon) of Bacillus thuringiensis vip 3Aa toxins

• Main Authors: Gamal H. Osman, Raya Soltane, Ibrahim Saleh, Hussein H. Abulreesh, Khaled S. Gazi, Ibrahim A. Arif, Ahmed M. Ramadan, Hussien F. Alameldin, Yehia A. Osman, Mamdouh Idriss
• Format: Article
• Language: English
• Published: Elsevier 2019-07-01
• Series: Saudi Journal of Biological Sciences
• Online Access: http://www.sciencedirect.com/science/article/pii/S1319562X18301438
• ISSN: 1319-562X

Black cutworm (BCW) is an economically important lepidopteran insect. The control of this insect by a Bt toxin and the understanding of the interaction between the Bt toxin and its receptor molecule were the objectives of this research work. A gene coding for a Vip3A receptor molecule was identified, characterized, and cloned, from the brush border membrane vesicles (BBMV) of the BCW. The nucleotide sequence analysis of the cloned putative Vip3A-receptor gene revealed that the gene was 1.3-kb long and exhibited no homology with any gene in the gene bank. We succeeded in identifying and characterizing most of the Vip3A-receptor gene sequence; and the nucleotide sequence analysis of the cloned putative Vip3A-receptor gene (accession no. KX858809) revealed about 92% of the expected sequence was recovered, which exhibited no homology with any gene in the GenBank. Keywords: Receptor, Vip3A, Bacillus thuringiensis, BBMV