Structural basis of ABCF-mediated resistance to pleuromutilin, lincosamide, and streptogramin A antibiotics in Gram-positive pathogens

Structural basis of ABCF-mediated resistance to pleuromutilin, lincosamide, and streptogramin A antibiotics in Gram-positive pathogens

Mitochondrial ribosomes (mitoribosomes) are characterized by a distinct architecture and thus biogenesis pathway. Here, cryo-EM structures of mitoribosome large subunit assembly intermediates elucidate final steps of 16 S rRNA folding, methylation and peptidyl transferase centre (PTC) completion, as...

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Main Authors: Caillan Crowe-McAuliffe, Victoriia Murina, Kathryn Jane Turnbull, Marje Kasari, Merianne Mohamad, Christine Polte, Hiraku Takada, Karolis Vaitkevicius, Jörgen Johansson, Zoya Ignatova, Gemma C. Atkinson, Alex J. O’Neill, Vasili Hauryliuk, Daniel N. Wilson
Format: Article
Language:English
Published: Nature Publishing Group 2021-06-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-021-23753-1
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spelling doaj-5839d6ab4dd34e3b9dd6b83dd05b0b332021-06-13T11:17:45ZengNature Publishing GroupNature Communications2041-17232021-06-0112111410.1038/s41467-021-23753-1Structural basis of ABCF-mediated resistance to pleuromutilin, lincosamide, and streptogramin A antibiotics in Gram-positive pathogensCaillan Crowe-McAuliffe0Victoriia Murina1Kathryn Jane Turnbull2Marje Kasari3Merianne Mohamad4Christine Polte5Hiraku Takada6Karolis Vaitkevicius7Jörgen Johansson8Zoya Ignatova9Gemma C. Atkinson10Alex J. O’Neill11Vasili Hauryliuk12Daniel N. Wilson13Institute for Biochemistry and Molecular Biology, University of HamburgDepartment of Molecular Biology, Umeå UniversityDepartment of Molecular Biology, Umeå UniversityUniversity of Tartu, Institute of TechnologyAstbury Centre for Structural Molecular Biology, School of Molecular & Cellular Biology, Faculty of Biological Sciences, University of LeedsInstitute for Biochemistry and Molecular Biology, University of HamburgDepartment of Molecular Biology, Umeå UniversityDepartment of Molecular Biology, Umeå UniversityDepartment of Molecular Biology, Umeå UniversityInstitute for Biochemistry and Molecular Biology, University of HamburgDepartment of Molecular Biology, Umeå UniversityAstbury Centre for Structural Molecular Biology, School of Molecular & Cellular Biology, Faculty of Biological Sciences, University of LeedsDepartment of Molecular Biology, Umeå UniversityInstitute for Biochemistry and Molecular Biology, University of HamburgMitochondrial ribosomes (mitoribosomes) are characterized by a distinct architecture and thus biogenesis pathway. Here, cryo-EM structures of mitoribosome large subunit assembly intermediates elucidate final steps of 16 S rRNA folding, methylation and peptidyl transferase centre (PTC) completion, as well as functions of several mitoribosome assembly factors.https://doi.org/10.1038/s41467-021-23753-1
collection DOAJ
language English
format Article
sources DOAJ
author Caillan Crowe-McAuliffe
Victoriia Murina
Kathryn Jane Turnbull
Marje Kasari
Merianne Mohamad
Christine Polte
Hiraku Takada
Karolis Vaitkevicius
Jörgen Johansson
Zoya Ignatova
Gemma C. Atkinson
Alex J. O’Neill
Vasili Hauryliuk
Daniel N. Wilson
spellingShingle Caillan Crowe-McAuliffe
Victoriia Murina
Kathryn Jane Turnbull
Marje Kasari
Merianne Mohamad
Christine Polte
Hiraku Takada
Karolis Vaitkevicius
Jörgen Johansson
Zoya Ignatova
Gemma C. Atkinson
Alex J. O’Neill
Vasili Hauryliuk
Daniel N. Wilson
Structural basis of ABCF-mediated resistance to pleuromutilin, lincosamide, and streptogramin A antibiotics in Gram-positive pathogens
Nature Communications
author_facet Caillan Crowe-McAuliffe
Victoriia Murina
Kathryn Jane Turnbull
Marje Kasari
Merianne Mohamad
Christine Polte
Hiraku Takada
Karolis Vaitkevicius
Jörgen Johansson
Zoya Ignatova
Gemma C. Atkinson
Alex J. O’Neill
Vasili Hauryliuk
Daniel N. Wilson
author_sort Caillan Crowe-McAuliffe
title Structural basis of ABCF-mediated resistance to pleuromutilin, lincosamide, and streptogramin A antibiotics in Gram-positive pathogens
title_short Structural basis of ABCF-mediated resistance to pleuromutilin, lincosamide, and streptogramin A antibiotics in Gram-positive pathogens
title_full Structural basis of ABCF-mediated resistance to pleuromutilin, lincosamide, and streptogramin A antibiotics in Gram-positive pathogens
title_fullStr Structural basis of ABCF-mediated resistance to pleuromutilin, lincosamide, and streptogramin A antibiotics in Gram-positive pathogens
title_full_unstemmed Structural basis of ABCF-mediated resistance to pleuromutilin, lincosamide, and streptogramin A antibiotics in Gram-positive pathogens
title_sort structural basis of abcf-mediated resistance to pleuromutilin, lincosamide, and streptogramin a antibiotics in gram-positive pathogens
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2021-06-01
description Mitochondrial ribosomes (mitoribosomes) are characterized by a distinct architecture and thus biogenesis pathway. Here, cryo-EM structures of mitoribosome large subunit assembly intermediates elucidate final steps of 16 S rRNA folding, methylation and peptidyl transferase centre (PTC) completion, as well as functions of several mitoribosome assembly factors.
url https://doi.org/10.1038/s41467-021-23753-1
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