ApoA-II modulates the association of HDL with class B scavenger receptors SR-BI and CD36
The class B scavenger receptors SR-BI and CD36 exhibit a broad ligand binding specificity. SR-BI is well characterized as a HDL receptor that mediates selective cholesteryl ester uptake from HDL. CD36, a receptor for oxidized LDL, also binds HDL and mediates selective cholesteryl ester uptake, altho...
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doaj-583f3c19afdf479cb0ec5afef3d4fded2021-04-27T04:41:00ZengElsevierJournal of Lipid Research0022-22752004-04-01454706715ApoA-II modulates the association of HDL with class B scavenger receptors SR-BI and CD36Maria C. de Beer0Lawrence W. Castellani1Lei Cai2Arnold J. Stromberg3Frederick C. de Beer4Deneys R. van der Westhuyzen5Department of Internal Medicine, University of Kentucky Medical Center, Lexington, KY 40536, and Department of Veterans Affairs Medical Center, Lexington, KY, 40511; Department of Medicine, University of California, Los Angeles, CA 90095; Department of Statistics, University of Kentucky, Lexington, KY 40506Department of Internal Medicine, University of Kentucky Medical Center, Lexington, KY 40536, and Department of Veterans Affairs Medical Center, Lexington, KY, 40511; Department of Medicine, University of California, Los Angeles, CA 90095; Department of Statistics, University of Kentucky, Lexington, KY 40506Department of Internal Medicine, University of Kentucky Medical Center, Lexington, KY 40536, and Department of Veterans Affairs Medical Center, Lexington, KY, 40511; Department of Medicine, University of California, Los Angeles, CA 90095; Department of Statistics, University of Kentucky, Lexington, KY 40506Department of Internal Medicine, University of Kentucky Medical Center, Lexington, KY 40536, and Department of Veterans Affairs Medical Center, Lexington, KY, 40511; Department of Medicine, University of California, Los Angeles, CA 90095; Department of Statistics, University of Kentucky, Lexington, KY 40506Department of Internal Medicine, University of Kentucky Medical Center, Lexington, KY 40536, and Department of Veterans Affairs Medical Center, Lexington, KY, 40511; Department of Medicine, University of California, Los Angeles, CA 90095; Department of Statistics, University of Kentucky, Lexington, KY 40506Department of Internal Medicine, University of Kentucky Medical Center, Lexington, KY 40536, and Department of Veterans Affairs Medical Center, Lexington, KY, 40511; Department of Medicine, University of California, Los Angeles, CA 90095; Department of Statistics, University of Kentucky, Lexington, KY 40506The class B scavenger receptors SR-BI and CD36 exhibit a broad ligand binding specificity. SR-BI is well characterized as a HDL receptor that mediates selective cholesteryl ester uptake from HDL. CD36, a receptor for oxidized LDL, also binds HDL and mediates selective cholesteryl ester uptake, although much less efficiently than SR-BI. Apolipoprotein A-II (apoA-II), the second most abundant HDL protein, is considered to be proatherogenic, but the underlying mechanisms are unclear. We previously showed that apoA-II modulates SR-BI-dependent binding and selective uptake of cholesteryl ester from reconstituted HDL. To investigate the effect of apoA-II in naturally occurring HDL on these processes, we compared HDL without apoA-II (from apoA-II null mice) with HDLs containing differing amounts of apoA-II (from C57BL/6 mice and transgenic mice expressing a mouse apoA-II transgene). The level of apoA-II in HDL was inversely correlated with HDL binding and selective cholesteryl ester uptake by both scavenger receptors, particularly CD36. Interestingly, for HDL lacking apoA-II, the efficiency with which CD36 mediated selective uptake reached a level similar to that of SR-BI.These results demonstrate that apoA-II exerts a marked effect on HDL binding and selective lipid uptake by the class B scavenger receptors and establishes a potentially important relationship between apoA-II and CD36.http://www.sciencedirect.com/science/article/pii/S0022227520318551lipoprotein metabolismselective lipid uptakeatherosclerosisapolipoprotein A-IIhigh density lipoprotein |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Maria C. de Beer Lawrence W. Castellani Lei Cai Arnold J. Stromberg Frederick C. de Beer Deneys R. van der Westhuyzen |
spellingShingle |
Maria C. de Beer Lawrence W. Castellani Lei Cai Arnold J. Stromberg Frederick C. de Beer Deneys R. van der Westhuyzen ApoA-II modulates the association of HDL with class B scavenger receptors SR-BI and CD36 Journal of Lipid Research lipoprotein metabolism selective lipid uptake atherosclerosis apolipoprotein A-II high density lipoprotein |
author_facet |
Maria C. de Beer Lawrence W. Castellani Lei Cai Arnold J. Stromberg Frederick C. de Beer Deneys R. van der Westhuyzen |
author_sort |
Maria C. de Beer |
title |
ApoA-II modulates the association of HDL with class B scavenger receptors SR-BI and CD36 |
title_short |
ApoA-II modulates the association of HDL with class B scavenger receptors SR-BI and CD36 |
title_full |
ApoA-II modulates the association of HDL with class B scavenger receptors SR-BI and CD36 |
title_fullStr |
ApoA-II modulates the association of HDL with class B scavenger receptors SR-BI and CD36 |
title_full_unstemmed |
ApoA-II modulates the association of HDL with class B scavenger receptors SR-BI and CD36 |
title_sort |
apoa-ii modulates the association of hdl with class b scavenger receptors sr-bi and cd36 |
publisher |
Elsevier |
series |
Journal of Lipid Research |
issn |
0022-2275 |
publishDate |
2004-04-01 |
description |
The class B scavenger receptors SR-BI and CD36 exhibit a broad ligand binding specificity. SR-BI is well characterized as a HDL receptor that mediates selective cholesteryl ester uptake from HDL. CD36, a receptor for oxidized LDL, also binds HDL and mediates selective cholesteryl ester uptake, although much less efficiently than SR-BI. Apolipoprotein A-II (apoA-II), the second most abundant HDL protein, is considered to be proatherogenic, but the underlying mechanisms are unclear. We previously showed that apoA-II modulates SR-BI-dependent binding and selective uptake of cholesteryl ester from reconstituted HDL. To investigate the effect of apoA-II in naturally occurring HDL on these processes, we compared HDL without apoA-II (from apoA-II null mice) with HDLs containing differing amounts of apoA-II (from C57BL/6 mice and transgenic mice expressing a mouse apoA-II transgene). The level of apoA-II in HDL was inversely correlated with HDL binding and selective cholesteryl ester uptake by both scavenger receptors, particularly CD36. Interestingly, for HDL lacking apoA-II, the efficiency with which CD36 mediated selective uptake reached a level similar to that of SR-BI.These results demonstrate that apoA-II exerts a marked effect on HDL binding and selective lipid uptake by the class B scavenger receptors and establishes a potentially important relationship between apoA-II and CD36. |
topic |
lipoprotein metabolism selective lipid uptake atherosclerosis apolipoprotein A-II high density lipoprotein |
url |
http://www.sciencedirect.com/science/article/pii/S0022227520318551 |
work_keys_str_mv |
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