The tree shrew is a promising model for the study of influenza B virus infection
Abstract Background Influenza B virus is a main causative pathogen of annual influenza epidemics, however, research on influenza B virus in general lags behind that on influenza A viruses, one of the important reasons is studies on influenza B viruses in animal models are limited. Here we investigat...
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2019-06-01
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Online Access: | http://link.springer.com/article/10.1186/s12985-019-1171-3 |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Bing Yuan Chunguang Yang Xueshan Xia Mark Zanin Sook-san Wong Fan Yang Jixiang Chang Zhitong Mai Jin Zhao Yunhui Zhang Runfeng Li Nanshan Zhong Zifeng Yang |
spellingShingle |
Bing Yuan Chunguang Yang Xueshan Xia Mark Zanin Sook-san Wong Fan Yang Jixiang Chang Zhitong Mai Jin Zhao Yunhui Zhang Runfeng Li Nanshan Zhong Zifeng Yang The tree shrew is a promising model for the study of influenza B virus infection Virology Journal Influenza B virus Tree shrew Ferret Mouse Animal model List of Abbreviations. |
author_facet |
Bing Yuan Chunguang Yang Xueshan Xia Mark Zanin Sook-san Wong Fan Yang Jixiang Chang Zhitong Mai Jin Zhao Yunhui Zhang Runfeng Li Nanshan Zhong Zifeng Yang |
author_sort |
Bing Yuan |
title |
The tree shrew is a promising model for the study of influenza B virus infection |
title_short |
The tree shrew is a promising model for the study of influenza B virus infection |
title_full |
The tree shrew is a promising model for the study of influenza B virus infection |
title_fullStr |
The tree shrew is a promising model for the study of influenza B virus infection |
title_full_unstemmed |
The tree shrew is a promising model for the study of influenza B virus infection |
title_sort |
tree shrew is a promising model for the study of influenza b virus infection |
publisher |
BMC |
series |
Virology Journal |
issn |
1743-422X |
publishDate |
2019-06-01 |
description |
Abstract Background Influenza B virus is a main causative pathogen of annual influenza epidemics, however, research on influenza B virus in general lags behind that on influenza A viruses, one of the important reasons is studies on influenza B viruses in animal models are limited. Here we investigated the tree shrew as a potential model for influenza B virus studies. Methods Tree shrews and ferrets were inoculated with either a Yamagata or Victoria lineage influenza B virus. Symptoms including nasal discharge and weight loss were observed. Nasal wash and respiratory tissues were collected at 2, 4 and 6 days post inoculation (DPI). Viral titers were measured in nasal washes and tissues were used for pathological examination and extraction of mRNA for measurement of cytokine expression. Results Clinical signs and pathological changes were also evident in the respiratory tracts of tree shrews and ferrets. Although nasal symptoms including sneezing and rhinorrhea were evident in ferrets infected with influenza B virus, tree shrews showed no significant respiratory symptoms, only milder nasal secretions appeared. Weight loss was observed in tree shrews but not ferrets. V0215 and Y12 replicated in all three animal (ferrets, tree shrews and mice) models with peak titers evident on 2DPI. There were no significant differences in peak viral titers in ferrets and tree shrews inoculated with Y12 at 2 and 4DPI, but viral titers were detected at 6DPI in tree shrews. Tree shrews infected with influenza B virus showed similar seroconversion and respiratory tract pathology to ferrets. Elevated levels of cytokines were detected in the tissues isolated from the respiratory tract after infection with either V0215 or Y12 compared to the levels in the uninfected control in both animals. Overall, the tree shrew was sensitive to infection and disease by influenza B virus. Conclusion The tree shrew to be a promising model for influenza B virus research. |
topic |
Influenza B virus Tree shrew Ferret Mouse Animal model List of Abbreviations. |
url |
http://link.springer.com/article/10.1186/s12985-019-1171-3 |
work_keys_str_mv |
AT bingyuan thetreeshrewisapromisingmodelforthestudyofinfluenzabvirusinfection AT chunguangyang thetreeshrewisapromisingmodelforthestudyofinfluenzabvirusinfection AT xueshanxia thetreeshrewisapromisingmodelforthestudyofinfluenzabvirusinfection AT markzanin thetreeshrewisapromisingmodelforthestudyofinfluenzabvirusinfection AT sooksanwong thetreeshrewisapromisingmodelforthestudyofinfluenzabvirusinfection AT fanyang thetreeshrewisapromisingmodelforthestudyofinfluenzabvirusinfection AT jixiangchang thetreeshrewisapromisingmodelforthestudyofinfluenzabvirusinfection AT zhitongmai thetreeshrewisapromisingmodelforthestudyofinfluenzabvirusinfection AT jinzhao thetreeshrewisapromisingmodelforthestudyofinfluenzabvirusinfection AT yunhuizhang thetreeshrewisapromisingmodelforthestudyofinfluenzabvirusinfection AT runfengli thetreeshrewisapromisingmodelforthestudyofinfluenzabvirusinfection AT nanshanzhong thetreeshrewisapromisingmodelforthestudyofinfluenzabvirusinfection AT zifengyang thetreeshrewisapromisingmodelforthestudyofinfluenzabvirusinfection AT bingyuan treeshrewisapromisingmodelforthestudyofinfluenzabvirusinfection AT chunguangyang treeshrewisapromisingmodelforthestudyofinfluenzabvirusinfection AT xueshanxia treeshrewisapromisingmodelforthestudyofinfluenzabvirusinfection AT markzanin treeshrewisapromisingmodelforthestudyofinfluenzabvirusinfection AT sooksanwong treeshrewisapromisingmodelforthestudyofinfluenzabvirusinfection AT fanyang treeshrewisapromisingmodelforthestudyofinfluenzabvirusinfection AT jixiangchang treeshrewisapromisingmodelforthestudyofinfluenzabvirusinfection AT zhitongmai treeshrewisapromisingmodelforthestudyofinfluenzabvirusinfection AT jinzhao treeshrewisapromisingmodelforthestudyofinfluenzabvirusinfection AT yunhuizhang treeshrewisapromisingmodelforthestudyofinfluenzabvirusinfection AT runfengli treeshrewisapromisingmodelforthestudyofinfluenzabvirusinfection AT nanshanzhong treeshrewisapromisingmodelforthestudyofinfluenzabvirusinfection AT zifengyang treeshrewisapromisingmodelforthestudyofinfluenzabvirusinfection |
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doaj-5854a0309fb74d7ca5a68351eb8e30fc2020-11-25T03:29:46ZengBMCVirology Journal1743-422X2019-06-0116111210.1186/s12985-019-1171-3The tree shrew is a promising model for the study of influenza B virus infectionBing Yuan0Chunguang Yang1Xueshan Xia2Mark Zanin3Sook-san Wong4Fan Yang5Jixiang Chang6Zhitong Mai7Jin Zhao8Yunhui Zhang9Runfeng Li10Nanshan Zhong11Zifeng Yang12Department of Respiration, the First People’s Hospital of Yunnan ProvinceState Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical UniversityFaculty of Life Science and Technology, Kunming University of Science And TechnologyState Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical UniversityState Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical UniversityMedical Faculty, Kunming University of Science And TechnologyMedical Faculty, Kunming University of Science And TechnologyState Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical UniversityState Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical UniversityDepartment of Respiration, the First People’s Hospital of Yunnan ProvinceState Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical UniversityState Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical UniversityState Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical UniversityAbstract Background Influenza B virus is a main causative pathogen of annual influenza epidemics, however, research on influenza B virus in general lags behind that on influenza A viruses, one of the important reasons is studies on influenza B viruses in animal models are limited. Here we investigated the tree shrew as a potential model for influenza B virus studies. Methods Tree shrews and ferrets were inoculated with either a Yamagata or Victoria lineage influenza B virus. Symptoms including nasal discharge and weight loss were observed. Nasal wash and respiratory tissues were collected at 2, 4 and 6 days post inoculation (DPI). Viral titers were measured in nasal washes and tissues were used for pathological examination and extraction of mRNA for measurement of cytokine expression. Results Clinical signs and pathological changes were also evident in the respiratory tracts of tree shrews and ferrets. Although nasal symptoms including sneezing and rhinorrhea were evident in ferrets infected with influenza B virus, tree shrews showed no significant respiratory symptoms, only milder nasal secretions appeared. Weight loss was observed in tree shrews but not ferrets. V0215 and Y12 replicated in all three animal (ferrets, tree shrews and mice) models with peak titers evident on 2DPI. There were no significant differences in peak viral titers in ferrets and tree shrews inoculated with Y12 at 2 and 4DPI, but viral titers were detected at 6DPI in tree shrews. Tree shrews infected with influenza B virus showed similar seroconversion and respiratory tract pathology to ferrets. Elevated levels of cytokines were detected in the tissues isolated from the respiratory tract after infection with either V0215 or Y12 compared to the levels in the uninfected control in both animals. Overall, the tree shrew was sensitive to infection and disease by influenza B virus. Conclusion The tree shrew to be a promising model for influenza B virus research.http://link.springer.com/article/10.1186/s12985-019-1171-3Influenza B virusTree shrewFerretMouseAnimal modelList of Abbreviations. |