Fluorescent Nanodiamonds Enable Long-Term Detection of Human Adipose-Derived Stem/Stromal Cells in an In Vivo Chondrogenesis Model Using Decellularized Extracellular Matrices and Fibrin Glue Polymer
Clinically available materials, including allogeneic irradiated costal cartilage and fibrin glue polymer, were used as scaffolds for in vivo chondrogenic differentiation of human adipose-derived stem/stromal cells (hASCs) in the attempt to develop a more efficient treatment over current methods. Cur...
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2019-08-01
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doaj-587b18dfa544489bb3eb0ea3ee048f2c2020-11-24T21:26:47ZengMDPI AGPolymers2073-43602019-08-01119139110.3390/polym11091391polym11091391Fluorescent Nanodiamonds Enable Long-Term Detection of Human Adipose-Derived Stem/Stromal Cells in an In Vivo Chondrogenesis Model Using Decellularized Extracellular Matrices and Fibrin Glue PolymerYi-Chia Wu0Ya-Chin Wang1Wei-Ting Wang2Hui-Min David Wang3Hsin-Hung Lin4Long-Jyun Su5Yur-Ren Kuo6Chung-Sheng Lai7Mei-Ling Ho8John Yu9Institute of Stem Cell and Translational Cancer Research, Chang Gung Memorial Hospital at Linko, Taoyuan 333, TaiwanRegeneration Medicine and Cell Therapy Research Center, Kaohsiung Medical University, Kaohsiung 807 TaiwanCenter of Teaching and Research, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung 801, TaiwanRegeneration Medicine and Cell Therapy Research Center, Kaohsiung Medical University, Kaohsiung 807 TaiwanInstitute of Atomic and Molecular Sciences, Academia Sinica, Taipei 106, TaiwanInstitute of Atomic and Molecular Sciences, Academia Sinica, Taipei 106, TaiwanRegeneration Medicine and Cell Therapy Research Center, Kaohsiung Medical University, Kaohsiung 807 TaiwanDivision of Plastic Surgery, Department of Surgery, Kaohsiung Medical University Hospital, Kaohsiung 807, TaiwanRegeneration Medicine and Cell Therapy Research Center, Kaohsiung Medical University, Kaohsiung 807 TaiwanInstitute of Stem Cell and Translational Cancer Research, Chang Gung Memorial Hospital at Linko, Taoyuan 333, TaiwanClinically available materials, including allogeneic irradiated costal cartilage and fibrin glue polymer, were used as scaffolds for in vivo chondrogenic differentiation of human adipose-derived stem/stromal cells (hASCs) in the attempt to develop a more efficient treatment over current methods. Current studies include the use of growth-factor stimulation, tissue engineering, and biocompatible materials; however, most methods involve complicated processes and pose clinical limitations. In this report, the xenografts in the experimental group composed of a diced decellularized cartilage extracellular matrix (ECM), hASCs, and fibrin glue polymer were implanted into the subcutaneous layer of nude mice, and the results were compared with two groups of controls; one control group received implantation of decellularized cartilage ECM and fibrin glue polymer, and the other control group received implantation of hASCs mixed with fibrin glue polymer. To evaluate whether hASCs had in vivo chondrogenesis in the xenografts, hASCs were labeled with fluorescent nanodiamonds (FNDs), a biocompatible and photostable nanomaterial, to allow for long-term detection and histological analysis. Increased cellularity, glycosaminoglycan, and collagen deposition were found by the histological examination in the experimental group compared with control groups. With the background-free detection technique and time-gated fluorescence imaging, the numbers and locations of the FND-labeled hASCs could be detected by confocal microscopy. The chondrocyte-specific markers, such as aggrecan and type II collagen, were colocalized with cells containing signals of FNDs which indicated in vivo chondrogenesis of hASCs. Taken together, functional in vivo chondrogenesis of the hASCs could be achieved by clinically available decellularized cartilage ECM and fibrin glue polymer in the nude mice model without in vitro chondrogenic induction. The fluorescent signals of FNDs in hASCs can be detected in histological analysis, such as hematoxylin and eosin staining (H&E staining) without the interference of the autofluorescence. Our study may warrant future clinical applications of the combination of decellular cartilage ECM, fibrin glue polymer, and hASCs for cartilage repair.https://www.mdpi.com/2073-4360/11/9/1391human adipose-derived stem/stromal cells (hASCs)in vivo chondrogenesisdecellularized cartilage ECMfibrin glue polymerfluorescent nanodiamonds (FNDs)long-term detection |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yi-Chia Wu Ya-Chin Wang Wei-Ting Wang Hui-Min David Wang Hsin-Hung Lin Long-Jyun Su Yur-Ren Kuo Chung-Sheng Lai Mei-Ling Ho John Yu |
spellingShingle |
Yi-Chia Wu Ya-Chin Wang Wei-Ting Wang Hui-Min David Wang Hsin-Hung Lin Long-Jyun Su Yur-Ren Kuo Chung-Sheng Lai Mei-Ling Ho John Yu Fluorescent Nanodiamonds Enable Long-Term Detection of Human Adipose-Derived Stem/Stromal Cells in an In Vivo Chondrogenesis Model Using Decellularized Extracellular Matrices and Fibrin Glue Polymer Polymers human adipose-derived stem/stromal cells (hASCs) in vivo chondrogenesis decellularized cartilage ECM fibrin glue polymer fluorescent nanodiamonds (FNDs) long-term detection |
author_facet |
Yi-Chia Wu Ya-Chin Wang Wei-Ting Wang Hui-Min David Wang Hsin-Hung Lin Long-Jyun Su Yur-Ren Kuo Chung-Sheng Lai Mei-Ling Ho John Yu |
author_sort |
Yi-Chia Wu |
title |
Fluorescent Nanodiamonds Enable Long-Term Detection of Human Adipose-Derived Stem/Stromal Cells in an In Vivo Chondrogenesis Model Using Decellularized Extracellular Matrices and Fibrin Glue Polymer |
title_short |
Fluorescent Nanodiamonds Enable Long-Term Detection of Human Adipose-Derived Stem/Stromal Cells in an In Vivo Chondrogenesis Model Using Decellularized Extracellular Matrices and Fibrin Glue Polymer |
title_full |
Fluorescent Nanodiamonds Enable Long-Term Detection of Human Adipose-Derived Stem/Stromal Cells in an In Vivo Chondrogenesis Model Using Decellularized Extracellular Matrices and Fibrin Glue Polymer |
title_fullStr |
Fluorescent Nanodiamonds Enable Long-Term Detection of Human Adipose-Derived Stem/Stromal Cells in an In Vivo Chondrogenesis Model Using Decellularized Extracellular Matrices and Fibrin Glue Polymer |
title_full_unstemmed |
Fluorescent Nanodiamonds Enable Long-Term Detection of Human Adipose-Derived Stem/Stromal Cells in an In Vivo Chondrogenesis Model Using Decellularized Extracellular Matrices and Fibrin Glue Polymer |
title_sort |
fluorescent nanodiamonds enable long-term detection of human adipose-derived stem/stromal cells in an in vivo chondrogenesis model using decellularized extracellular matrices and fibrin glue polymer |
publisher |
MDPI AG |
series |
Polymers |
issn |
2073-4360 |
publishDate |
2019-08-01 |
description |
Clinically available materials, including allogeneic irradiated costal cartilage and fibrin glue polymer, were used as scaffolds for in vivo chondrogenic differentiation of human adipose-derived stem/stromal cells (hASCs) in the attempt to develop a more efficient treatment over current methods. Current studies include the use of growth-factor stimulation, tissue engineering, and biocompatible materials; however, most methods involve complicated processes and pose clinical limitations. In this report, the xenografts in the experimental group composed of a diced decellularized cartilage extracellular matrix (ECM), hASCs, and fibrin glue polymer were implanted into the subcutaneous layer of nude mice, and the results were compared with two groups of controls; one control group received implantation of decellularized cartilage ECM and fibrin glue polymer, and the other control group received implantation of hASCs mixed with fibrin glue polymer. To evaluate whether hASCs had in vivo chondrogenesis in the xenografts, hASCs were labeled with fluorescent nanodiamonds (FNDs), a biocompatible and photostable nanomaterial, to allow for long-term detection and histological analysis. Increased cellularity, glycosaminoglycan, and collagen deposition were found by the histological examination in the experimental group compared with control groups. With the background-free detection technique and time-gated fluorescence imaging, the numbers and locations of the FND-labeled hASCs could be detected by confocal microscopy. The chondrocyte-specific markers, such as aggrecan and type II collagen, were colocalized with cells containing signals of FNDs which indicated in vivo chondrogenesis of hASCs. Taken together, functional in vivo chondrogenesis of the hASCs could be achieved by clinically available decellularized cartilage ECM and fibrin glue polymer in the nude mice model without in vitro chondrogenic induction. The fluorescent signals of FNDs in hASCs can be detected in histological analysis, such as hematoxylin and eosin staining (H&E staining) without the interference of the autofluorescence. Our study may warrant future clinical applications of the combination of decellular cartilage ECM, fibrin glue polymer, and hASCs for cartilage repair. |
topic |
human adipose-derived stem/stromal cells (hASCs) in vivo chondrogenesis decellularized cartilage ECM fibrin glue polymer fluorescent nanodiamonds (FNDs) long-term detection |
url |
https://www.mdpi.com/2073-4360/11/9/1391 |
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