| Summary: | Pregnadiene-11-hydroxy-16α,17α-epoxy-3,20-dione-1 (PYED-1), a heterocyclic corticosteroid derivative of deflazacort, exhibits broad-spectrum antibacterial activity against Gram-negative and Gram-positive bacteria. Here, we investigated the effect of PYED-1 on the biofilms of <i>Staphylococcus aureus</i>, an etiological agent of biofilm-based chronic infections such as osteomyelitis, indwelling medical device infections, periodontitis, chronic wound infections, and endocarditis. PYED-1 caused a strong reduction in biofilm formation in a concentration dependent manner. Furthermore, it was also able to completely remove the preformed biofilm. Transcriptional analysis performed on the established biofilm revealed that PYED-1 downregulates the expression of genes related to quorum sensing (<i>agrA</i>, <i>RNAIII</i>, <i>hld</i>, <i>psm</i>, and <i>sarA</i>), surface proteins (<i>clfB</i> and <i>fnbB</i>), secreted toxins (<i>hla</i>, <i>hlb</i><i>, </i>and<i> </i><i>lukD</i>), and capsular polysaccharides (<i>capC</i>). The expression of genes that encode two main global regulators, <i>sigB</i> and <i>saeR</i>, was also significantly inhibited after treatment with PYED-1. In conclusion, PYED-1 not only effectively inhibited biofilm formation, but also eradicated preformed biofilms of <i>S. aureus</i>, modulating the expression of genes related to quorum sensing, surface and secreted proteins, and capsular polysaccharides. These results indicated that PYED-1 may have great potential as an effective antibiofilm agent to prevent<i> </i><i>S. aureus</i> biofilm-associated infections.
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