PYED-1 Inhibits Biofilm Formation and Disrupts the Preformed Biofilm of <i>Staphylococcus aureus</i>
Pregnadiene-11-hydroxy-16α,17α-epoxy-3,20-dione-1 (PYED-1), a heterocyclic corticosteroid derivative of deflazacort, exhibits broad-spectrum antibacterial activity against Gram-negative and Gram-positive bacteria. Here, we investigated the effect of PYED-1 on the biofilms of <i>Staphylococcus...
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doaj-58825b583efd4120822f0b99b87101912020-11-25T02:04:33ZengMDPI AGAntibiotics2079-63822020-05-01924024010.3390/antibiotics9050240PYED-1 Inhibits Biofilm Formation and Disrupts the Preformed Biofilm of <i>Staphylococcus aureus</i>Adriana Vollaro0Anna Esposito1Eliana Pia Esposito2Raffaele Zarrilli3Annalisa Guaragna4Eliana De Gregorio5Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, 80131 Naples, ItalyDepartment of Chemical Sciences, University of Naples Federico II, 80126 Naples, ItalyDepartment of Public Health, University of Naples Federico II, 80131 Naples, ItalyDepartment of Public Health, University of Naples Federico II, 80131 Naples, ItalyDepartment of Chemical Sciences, University of Naples Federico II, 80126 Naples, ItalyDepartment of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, 80131 Naples, ItalyPregnadiene-11-hydroxy-16α,17α-epoxy-3,20-dione-1 (PYED-1), a heterocyclic corticosteroid derivative of deflazacort, exhibits broad-spectrum antibacterial activity against Gram-negative and Gram-positive bacteria. Here, we investigated the effect of PYED-1 on the biofilms of <i>Staphylococcus aureus</i>, an etiological agent of biofilm-based chronic infections such as osteomyelitis, indwelling medical device infections, periodontitis, chronic wound infections, and endocarditis. PYED-1 caused a strong reduction in biofilm formation in a concentration dependent manner. Furthermore, it was also able to completely remove the preformed biofilm. Transcriptional analysis performed on the established biofilm revealed that PYED-1 downregulates the expression of genes related to quorum sensing (<i>agrA</i>, <i>RNAIII</i>, <i>hld</i>, <i>psm</i>, and <i>sarA</i>), surface proteins (<i>clfB</i> and <i>fnbB</i>), secreted toxins (<i>hla</i>, <i>hlb</i><i>, </i>and<i> </i><i>lukD</i>), and capsular polysaccharides (<i>capC</i>). The expression of genes that encode two main global regulators, <i>sigB</i> and <i>saeR</i>, was also significantly inhibited after treatment with PYED-1. In conclusion, PYED-1 not only effectively inhibited biofilm formation, but also eradicated preformed biofilms of <i>S. aureus</i>, modulating the expression of genes related to quorum sensing, surface and secreted proteins, and capsular polysaccharides. These results indicated that PYED-1 may have great potential as an effective antibiofilm agent to prevent<i> </i><i>S. aureus</i> biofilm-associated infections.https://www.mdpi.com/2079-6382/9/5/240biofilm formationpreformed biofilmantibiofilm activitybiofilm eradication agentStaphylococcus aureuscorticosteroid |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Adriana Vollaro Anna Esposito Eliana Pia Esposito Raffaele Zarrilli Annalisa Guaragna Eliana De Gregorio |
spellingShingle |
Adriana Vollaro Anna Esposito Eliana Pia Esposito Raffaele Zarrilli Annalisa Guaragna Eliana De Gregorio PYED-1 Inhibits Biofilm Formation and Disrupts the Preformed Biofilm of <i>Staphylococcus aureus</i> Antibiotics biofilm formation preformed biofilm antibiofilm activity biofilm eradication agent Staphylococcus aureus corticosteroid |
author_facet |
Adriana Vollaro Anna Esposito Eliana Pia Esposito Raffaele Zarrilli Annalisa Guaragna Eliana De Gregorio |
author_sort |
Adriana Vollaro |
title |
PYED-1 Inhibits Biofilm Formation and Disrupts the Preformed Biofilm of <i>Staphylococcus aureus</i> |
title_short |
PYED-1 Inhibits Biofilm Formation and Disrupts the Preformed Biofilm of <i>Staphylococcus aureus</i> |
title_full |
PYED-1 Inhibits Biofilm Formation and Disrupts the Preformed Biofilm of <i>Staphylococcus aureus</i> |
title_fullStr |
PYED-1 Inhibits Biofilm Formation and Disrupts the Preformed Biofilm of <i>Staphylococcus aureus</i> |
title_full_unstemmed |
PYED-1 Inhibits Biofilm Formation and Disrupts the Preformed Biofilm of <i>Staphylococcus aureus</i> |
title_sort |
pyed-1 inhibits biofilm formation and disrupts the preformed biofilm of <i>staphylococcus aureus</i> |
publisher |
MDPI AG |
series |
Antibiotics |
issn |
2079-6382 |
publishDate |
2020-05-01 |
description |
Pregnadiene-11-hydroxy-16α,17α-epoxy-3,20-dione-1 (PYED-1), a heterocyclic corticosteroid derivative of deflazacort, exhibits broad-spectrum antibacterial activity against Gram-negative and Gram-positive bacteria. Here, we investigated the effect of PYED-1 on the biofilms of <i>Staphylococcus aureus</i>, an etiological agent of biofilm-based chronic infections such as osteomyelitis, indwelling medical device infections, periodontitis, chronic wound infections, and endocarditis. PYED-1 caused a strong reduction in biofilm formation in a concentration dependent manner. Furthermore, it was also able to completely remove the preformed biofilm. Transcriptional analysis performed on the established biofilm revealed that PYED-1 downregulates the expression of genes related to quorum sensing (<i>agrA</i>, <i>RNAIII</i>, <i>hld</i>, <i>psm</i>, and <i>sarA</i>), surface proteins (<i>clfB</i> and <i>fnbB</i>), secreted toxins (<i>hla</i>, <i>hlb</i><i>, </i>and<i> </i><i>lukD</i>), and capsular polysaccharides (<i>capC</i>). The expression of genes that encode two main global regulators, <i>sigB</i> and <i>saeR</i>, was also significantly inhibited after treatment with PYED-1. In conclusion, PYED-1 not only effectively inhibited biofilm formation, but also eradicated preformed biofilms of <i>S. aureus</i>, modulating the expression of genes related to quorum sensing, surface and secreted proteins, and capsular polysaccharides. These results indicated that PYED-1 may have great potential as an effective antibiofilm agent to prevent<i> </i><i>S. aureus</i> biofilm-associated infections. |
topic |
biofilm formation preformed biofilm antibiofilm activity biofilm eradication agent Staphylococcus aureus corticosteroid |
url |
https://www.mdpi.com/2079-6382/9/5/240 |
work_keys_str_mv |
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