Antibacterial Activity of Chromomycins from a Marine-Derived <i>Streptomyces microflavus</i>
A marine-derived actinomycete (<i>Streptomyces</i> sp. MBTI36) exhibiting antibacterial activities was investigated in the present study. The strain was identified using genetic techniques. The 16S rDNA sequence of the isolate indicated that it was most closely related to <i>Strept...
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doaj-588b37137e6044f78403f192998c986a2020-11-25T03:03:53ZengMDPI AGMarine Drugs1660-33972020-10-011852252210.3390/md18100522Antibacterial Activity of Chromomycins from a Marine-Derived <i>Streptomyces microflavus</i>Eunji Cho0Oh-Seok Kwon1Beomkoo Chung2Jayho Lee3Jeongyoon Sun4Jongheon Shin5Ki-Bong Oh6Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, KoreaNatural Products Research Institute, College of Pharmacy, Seoul National University, Seoul 08826, KoreaDepartment of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, KoreaDepartment of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, KoreaDepartment of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, KoreaNatural Products Research Institute, College of Pharmacy, Seoul National University, Seoul 08826, KoreaDepartment of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, KoreaA marine-derived actinomycete (<i>Streptomyces</i> sp. MBTI36) exhibiting antibacterial activities was investigated in the present study. The strain was identified using genetic techniques. The 16S rDNA sequence of the isolate indicated that it was most closely related to <i>Streptomyces microflavus</i>. Furthermore, a new chromomycin A<sub>9</sub> (<b>1</b>), along with chromomycin Ap (<b>2</b>), chromomycin A<sub>2</sub> (<b>3</b>), and chromomycin A<sub>3</sub> (<b>4</b>), were isolated from the ethyl acetate extract. Their structures were determined using extensive spectroscopic methods including 1D and 2D NMR, and HRMS, as well as comparisons with previously reported data. Compounds <b>1</b>–<b>4 </b>showed potent antibacterial activities against Gram-positive bacteria including methicillin-resistant <i>Staphylococcus aureus</i> (MRSA). During a passage experiment, minimum inhibitory concentration (MIC) values for compounds <b>1</b>–<b>4 </b>showed no more than a 4-fold increase from the starting MIC value, indicating that no resistance was detected over the 21 passages.https://www.mdpi.com/1660-3397/18/10/522chromomycinsmarine actinomycete<i>Streptomyces microflavus</i>antimicrobial activityMRSAresistance |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Eunji Cho Oh-Seok Kwon Beomkoo Chung Jayho Lee Jeongyoon Sun Jongheon Shin Ki-Bong Oh |
spellingShingle |
Eunji Cho Oh-Seok Kwon Beomkoo Chung Jayho Lee Jeongyoon Sun Jongheon Shin Ki-Bong Oh Antibacterial Activity of Chromomycins from a Marine-Derived <i>Streptomyces microflavus</i> Marine Drugs chromomycins marine actinomycete <i>Streptomyces microflavus</i> antimicrobial activity MRSA resistance |
author_facet |
Eunji Cho Oh-Seok Kwon Beomkoo Chung Jayho Lee Jeongyoon Sun Jongheon Shin Ki-Bong Oh |
author_sort |
Eunji Cho |
title |
Antibacterial Activity of Chromomycins from a Marine-Derived <i>Streptomyces microflavus</i> |
title_short |
Antibacterial Activity of Chromomycins from a Marine-Derived <i>Streptomyces microflavus</i> |
title_full |
Antibacterial Activity of Chromomycins from a Marine-Derived <i>Streptomyces microflavus</i> |
title_fullStr |
Antibacterial Activity of Chromomycins from a Marine-Derived <i>Streptomyces microflavus</i> |
title_full_unstemmed |
Antibacterial Activity of Chromomycins from a Marine-Derived <i>Streptomyces microflavus</i> |
title_sort |
antibacterial activity of chromomycins from a marine-derived <i>streptomyces microflavus</i> |
publisher |
MDPI AG |
series |
Marine Drugs |
issn |
1660-3397 |
publishDate |
2020-10-01 |
description |
A marine-derived actinomycete (<i>Streptomyces</i> sp. MBTI36) exhibiting antibacterial activities was investigated in the present study. The strain was identified using genetic techniques. The 16S rDNA sequence of the isolate indicated that it was most closely related to <i>Streptomyces microflavus</i>. Furthermore, a new chromomycin A<sub>9</sub> (<b>1</b>), along with chromomycin Ap (<b>2</b>), chromomycin A<sub>2</sub> (<b>3</b>), and chromomycin A<sub>3</sub> (<b>4</b>), were isolated from the ethyl acetate extract. Their structures were determined using extensive spectroscopic methods including 1D and 2D NMR, and HRMS, as well as comparisons with previously reported data. Compounds <b>1</b>–<b>4 </b>showed potent antibacterial activities against Gram-positive bacteria including methicillin-resistant <i>Staphylococcus aureus</i> (MRSA). During a passage experiment, minimum inhibitory concentration (MIC) values for compounds <b>1</b>–<b>4 </b>showed no more than a 4-fold increase from the starting MIC value, indicating that no resistance was detected over the 21 passages. |
topic |
chromomycins marine actinomycete <i>Streptomyces microflavus</i> antimicrobial activity MRSA resistance |
url |
https://www.mdpi.com/1660-3397/18/10/522 |
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