Non-Invasive Characterization of the Pancreas During Bariatric Surgery via Circulating Pancreatic Specific Cell-free Messenger RNA
Bariatric surgery results in sustained weight loss and improvement in glucose homeostasis. However, the lack of accessible non-invasive tools to examine molecular alterations occurring in the pancreas limits our understanding of the causes and recovery of glucose homeostasis. Here, we describe the u...
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Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2021.742496/full |
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doaj-588ca370372f4a01bc4f9f2159836d9a2021-10-11T04:30:39ZengFrontiers Media S.A.Frontiers in Genetics1664-80212021-10-011210.3389/fgene.2021.742496742496Non-Invasive Characterization of the Pancreas During Bariatric Surgery via Circulating Pancreatic Specific Cell-free Messenger RNAKong Kiat Whye0E ShyongTai1E ShyongTai2Asim Shabbir3Asim Shabbir4Chin Meng Khoo5Chin Meng Khoo6Winston Koh7Molecular Engineering Lab, Institute of Molecular and Cell Biology, A*STAR, Singapore, SingaporeDivision of Endocrinology, Department of Medicine, National University Hospital, Singapore, SingaporeYong Loo Lin School of Medicine, National University of Singapore, Singapore, SingaporeYong Loo Lin School of Medicine, National University of Singapore, Singapore, SingaporeDepartment of Surgery, National University Hospital, Singapore, SingaporeDivision of Endocrinology, Department of Medicine, National University Hospital, Singapore, SingaporeYong Loo Lin School of Medicine, National University of Singapore, Singapore, SingaporeMolecular Engineering Lab, Institute of Molecular and Cell Biology, A*STAR, Singapore, SingaporeBariatric surgery results in sustained weight loss and improvement in glucose homeostasis. However, the lack of accessible non-invasive tools to examine molecular alterations occurring in the pancreas limits our understanding of the causes and recovery of glucose homeostasis. Here, we describe the use of a circulating cell free mRNA (cfmRNA) based multiplex qPCR assay to selectively amplify and quantify circulating pancreatic specific transcripts levels within plasma. We applied this assay to a cohort of 58 plasma samples consisting of 10 patients that tracks multiple time points including pre and post-bariatric surgery. In our targeted multiplex screen of 14 selected pancreatic specific circulating transcripts, we identified 13 pancreatic specific transcripts that can be amplified from plasma. Furthermore, when quantifying the amplicons obtained in the short-term post-surgery (2 weeks–1 month) and long-term (3–12 months), we observed a consistent reduction of circulating GCG transcripts during short term post-surgery. Across the cohort, GCG cfmRNA levels correlated significantly with common metrics of improvement following bariatric surgery such as: haemoglobin A1c levels (R: −0.41, p-value: 0.0039) and percentage of excess weight loss (R: 0.29, p-value: 0.046).https://www.frontiersin.org/articles/10.3389/fgene.2021.742496/fullcfmRNAliquid biospsybariatric surgerypancreasmetabolic health |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kong Kiat Whye E ShyongTai E ShyongTai Asim Shabbir Asim Shabbir Chin Meng Khoo Chin Meng Khoo Winston Koh |
spellingShingle |
Kong Kiat Whye E ShyongTai E ShyongTai Asim Shabbir Asim Shabbir Chin Meng Khoo Chin Meng Khoo Winston Koh Non-Invasive Characterization of the Pancreas During Bariatric Surgery via Circulating Pancreatic Specific Cell-free Messenger RNA Frontiers in Genetics cfmRNA liquid biospsy bariatric surgery pancreas metabolic health |
author_facet |
Kong Kiat Whye E ShyongTai E ShyongTai Asim Shabbir Asim Shabbir Chin Meng Khoo Chin Meng Khoo Winston Koh |
author_sort |
Kong Kiat Whye |
title |
Non-Invasive Characterization of the Pancreas During Bariatric Surgery via Circulating Pancreatic Specific Cell-free Messenger RNA |
title_short |
Non-Invasive Characterization of the Pancreas During Bariatric Surgery via Circulating Pancreatic Specific Cell-free Messenger RNA |
title_full |
Non-Invasive Characterization of the Pancreas During Bariatric Surgery via Circulating Pancreatic Specific Cell-free Messenger RNA |
title_fullStr |
Non-Invasive Characterization of the Pancreas During Bariatric Surgery via Circulating Pancreatic Specific Cell-free Messenger RNA |
title_full_unstemmed |
Non-Invasive Characterization of the Pancreas During Bariatric Surgery via Circulating Pancreatic Specific Cell-free Messenger RNA |
title_sort |
non-invasive characterization of the pancreas during bariatric surgery via circulating pancreatic specific cell-free messenger rna |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Genetics |
issn |
1664-8021 |
publishDate |
2021-10-01 |
description |
Bariatric surgery results in sustained weight loss and improvement in glucose homeostasis. However, the lack of accessible non-invasive tools to examine molecular alterations occurring in the pancreas limits our understanding of the causes and recovery of glucose homeostasis. Here, we describe the use of a circulating cell free mRNA (cfmRNA) based multiplex qPCR assay to selectively amplify and quantify circulating pancreatic specific transcripts levels within plasma. We applied this assay to a cohort of 58 plasma samples consisting of 10 patients that tracks multiple time points including pre and post-bariatric surgery. In our targeted multiplex screen of 14 selected pancreatic specific circulating transcripts, we identified 13 pancreatic specific transcripts that can be amplified from plasma. Furthermore, when quantifying the amplicons obtained in the short-term post-surgery (2 weeks–1 month) and long-term (3–12 months), we observed a consistent reduction of circulating GCG transcripts during short term post-surgery. Across the cohort, GCG cfmRNA levels correlated significantly with common metrics of improvement following bariatric surgery such as: haemoglobin A1c levels (R: −0.41, p-value: 0.0039) and percentage of excess weight loss (R: 0.29, p-value: 0.046). |
topic |
cfmRNA liquid biospsy bariatric surgery pancreas metabolic health |
url |
https://www.frontiersin.org/articles/10.3389/fgene.2021.742496/full |
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