Modified hyaluronic acid hydrogels with chemical groups that facilitate adhesion to host tissues enhance cartilage regeneration
Stable integration of hydrogel implants with host tissues is of critical importance to cartilage tissue engineering. Designing and fabricating hydrogels with high adhesive strength, stability and regeneration potential are major challenges to be overcome. This study fabricated injectable adhesive hy...
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KeAi Communications Co., Ltd.
2021-06-01
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doaj-5891584eaa6d43b0b5f3ea4b7b8020132021-03-22T12:51:30ZengKeAi Communications Co., Ltd.Bioactive Materials2452-199X2021-06-016616891698Modified hyaluronic acid hydrogels with chemical groups that facilitate adhesion to host tissues enhance cartilage regenerationJiaqing Chen0Jiabei Yang1Li Wang2Xuewei Zhang3Boon Chin Heng4Dong-An Wang5Zigang Ge6Department of Biomedical Engineering, College of Engineering, Peking University, Beijing, 100871, PR ChinaDepartment of Biomedical Engineering, College of Engineering, Peking University, Beijing, 100871, PR ChinaDepartment of Biomedical Engineering, College of Engineering, Peking University, Beijing, 100871, PR ChinaDepartment of Biomedical Engineering, College of Engineering, Peking University, Beijing, 100871, PR ChinaSchool of Stomatology, Peking University, Beijing, 100081, PR ChinaDepartment of Biomedical Engineering, College of Engineering, City University of Hong Kong, Hong Kong SAR, PR ChinaDepartment of Biomedical Engineering, College of Engineering, Peking University, Beijing, 100871, PR China; Corresponding author.Stable integration of hydrogel implants with host tissues is of critical importance to cartilage tissue engineering. Designing and fabricating hydrogels with high adhesive strength, stability and regeneration potential are major challenges to be overcome. This study fabricated injectable adhesive hyaluronic acid (HA) hydrogel modified by aldehyde groups and methacrylate (AHAMA) on the polysaccharide backbone with multiple anchoring mechanisms (amide bond through the dynamic Schiff base reaction, hydrogen bond and physical interpenetration). AHAMA hydrogel exhibited significantly improved durability and stability within a humid environment (at least 7 days), together with higher adhesive strength (43 KPa to skin and 52 KPa to glass), as compared to commercial fibrin glue (nearly 10 KPa) and HAMA hydrogel (nearly 20 KPa). The results showed that AHAMA hydrogel was biocompatible and could be easily and rapidly prepared in situ. In vitro cell culture experiments showed that AHAMA hydrogel could enhance proliferation (1.2-folds after 3 days) and migration (1.5-folds after 12 h) of bone marrow stem cells (BMSCs), as compared to cells cultured in a culture dish. Furthermore, in a rat osteochondral defect model, implanted AHAMA hydrogel significantly promoted integration between neo-cartilage and host tissues, and significantly improved cartilage regeneration (modified O'Driscoll histological scores of 16.0 ± 4.1 and 18.3 ± 4.6 after 4 and 12-weeks of post-implantation in AHAMA groups respectively, 12.0 ± 2.7 and 12.2 ± 2.8 respectively in HAMA groups, 9.8 ± 2.4 and 11.5 ± 2.1 respectively in untreated groups). Hence, AHAMA hydrogel is a promising adhesive biomaterial for clinical cartilage regeneration and other biomedical applications.http://www.sciencedirect.com/science/article/pii/S2452199X20303078Cartilage regenerationHydrogelAdhesionSchiff base reactionHyaluronic acidAldehyde |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jiaqing Chen Jiabei Yang Li Wang Xuewei Zhang Boon Chin Heng Dong-An Wang Zigang Ge |
spellingShingle |
Jiaqing Chen Jiabei Yang Li Wang Xuewei Zhang Boon Chin Heng Dong-An Wang Zigang Ge Modified hyaluronic acid hydrogels with chemical groups that facilitate adhesion to host tissues enhance cartilage regeneration Bioactive Materials Cartilage regeneration Hydrogel Adhesion Schiff base reaction Hyaluronic acid Aldehyde |
author_facet |
Jiaqing Chen Jiabei Yang Li Wang Xuewei Zhang Boon Chin Heng Dong-An Wang Zigang Ge |
author_sort |
Jiaqing Chen |
title |
Modified hyaluronic acid hydrogels with chemical groups that facilitate adhesion to host tissues enhance cartilage regeneration |
title_short |
Modified hyaluronic acid hydrogels with chemical groups that facilitate adhesion to host tissues enhance cartilage regeneration |
title_full |
Modified hyaluronic acid hydrogels with chemical groups that facilitate adhesion to host tissues enhance cartilage regeneration |
title_fullStr |
Modified hyaluronic acid hydrogels with chemical groups that facilitate adhesion to host tissues enhance cartilage regeneration |
title_full_unstemmed |
Modified hyaluronic acid hydrogels with chemical groups that facilitate adhesion to host tissues enhance cartilage regeneration |
title_sort |
modified hyaluronic acid hydrogels with chemical groups that facilitate adhesion to host tissues enhance cartilage regeneration |
publisher |
KeAi Communications Co., Ltd. |
series |
Bioactive Materials |
issn |
2452-199X |
publishDate |
2021-06-01 |
description |
Stable integration of hydrogel implants with host tissues is of critical importance to cartilage tissue engineering. Designing and fabricating hydrogels with high adhesive strength, stability and regeneration potential are major challenges to be overcome. This study fabricated injectable adhesive hyaluronic acid (HA) hydrogel modified by aldehyde groups and methacrylate (AHAMA) on the polysaccharide backbone with multiple anchoring mechanisms (amide bond through the dynamic Schiff base reaction, hydrogen bond and physical interpenetration). AHAMA hydrogel exhibited significantly improved durability and stability within a humid environment (at least 7 days), together with higher adhesive strength (43 KPa to skin and 52 KPa to glass), as compared to commercial fibrin glue (nearly 10 KPa) and HAMA hydrogel (nearly 20 KPa). The results showed that AHAMA hydrogel was biocompatible and could be easily and rapidly prepared in situ. In vitro cell culture experiments showed that AHAMA hydrogel could enhance proliferation (1.2-folds after 3 days) and migration (1.5-folds after 12 h) of bone marrow stem cells (BMSCs), as compared to cells cultured in a culture dish. Furthermore, in a rat osteochondral defect model, implanted AHAMA hydrogel significantly promoted integration between neo-cartilage and host tissues, and significantly improved cartilage regeneration (modified O'Driscoll histological scores of 16.0 ± 4.1 and 18.3 ± 4.6 after 4 and 12-weeks of post-implantation in AHAMA groups respectively, 12.0 ± 2.7 and 12.2 ± 2.8 respectively in HAMA groups, 9.8 ± 2.4 and 11.5 ± 2.1 respectively in untreated groups). Hence, AHAMA hydrogel is a promising adhesive biomaterial for clinical cartilage regeneration and other biomedical applications. |
topic |
Cartilage regeneration Hydrogel Adhesion Schiff base reaction Hyaluronic acid Aldehyde |
url |
http://www.sciencedirect.com/science/article/pii/S2452199X20303078 |
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