Development and evaluation of a rapid CRISPR-based diagnostic for COVID-19.
The recent outbreak of human infections caused by SARS-CoV-2, the third zoonotic coronavirus has raised great public health concern globally. Rapid and accurate diagnosis of this novel pathogen posts great challenges not only clinically but also technologically. Metagenomic next-generation sequencin...
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2020-08-01
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Online Access: | https://doi.org/10.1371/journal.ppat.1008705 |
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doaj-58957edba2c6476488228001794581e82021-04-21T17:42:41ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742020-08-01168e100870510.1371/journal.ppat.1008705Development and evaluation of a rapid CRISPR-based diagnostic for COVID-19.Tieying HouWeiqi ZengMinling YangWenjing ChenLili RenJingwen AiJi WuYalong LiaoXuejing GouYongjun LiXiaorui WangHang SuBing GuJianwei WangTeng XuThe recent outbreak of human infections caused by SARS-CoV-2, the third zoonotic coronavirus has raised great public health concern globally. Rapid and accurate diagnosis of this novel pathogen posts great challenges not only clinically but also technologically. Metagenomic next-generation sequencing (mNGS) and reverse-transcription PCR (RT-PCR) have been the most commonly used molecular methodologies. However, each has their own limitations. In this study, we developed an isothermal, CRISPR-based diagnostic for COVID-19 with near single-copy sensitivity. The diagnostic performances of all three technology platforms were also compared. Our study aimed to provide more insights into the molecular detection of SARS-CoV-2, and also to present a novel diagnostic option for this new emerging virus.https://doi.org/10.1371/journal.ppat.1008705 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tieying Hou Weiqi Zeng Minling Yang Wenjing Chen Lili Ren Jingwen Ai Ji Wu Yalong Liao Xuejing Gou Yongjun Li Xiaorui Wang Hang Su Bing Gu Jianwei Wang Teng Xu |
spellingShingle |
Tieying Hou Weiqi Zeng Minling Yang Wenjing Chen Lili Ren Jingwen Ai Ji Wu Yalong Liao Xuejing Gou Yongjun Li Xiaorui Wang Hang Su Bing Gu Jianwei Wang Teng Xu Development and evaluation of a rapid CRISPR-based diagnostic for COVID-19. PLoS Pathogens |
author_facet |
Tieying Hou Weiqi Zeng Minling Yang Wenjing Chen Lili Ren Jingwen Ai Ji Wu Yalong Liao Xuejing Gou Yongjun Li Xiaorui Wang Hang Su Bing Gu Jianwei Wang Teng Xu |
author_sort |
Tieying Hou |
title |
Development and evaluation of a rapid CRISPR-based diagnostic for COVID-19. |
title_short |
Development and evaluation of a rapid CRISPR-based diagnostic for COVID-19. |
title_full |
Development and evaluation of a rapid CRISPR-based diagnostic for COVID-19. |
title_fullStr |
Development and evaluation of a rapid CRISPR-based diagnostic for COVID-19. |
title_full_unstemmed |
Development and evaluation of a rapid CRISPR-based diagnostic for COVID-19. |
title_sort |
development and evaluation of a rapid crispr-based diagnostic for covid-19. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS Pathogens |
issn |
1553-7366 1553-7374 |
publishDate |
2020-08-01 |
description |
The recent outbreak of human infections caused by SARS-CoV-2, the third zoonotic coronavirus has raised great public health concern globally. Rapid and accurate diagnosis of this novel pathogen posts great challenges not only clinically but also technologically. Metagenomic next-generation sequencing (mNGS) and reverse-transcription PCR (RT-PCR) have been the most commonly used molecular methodologies. However, each has their own limitations. In this study, we developed an isothermal, CRISPR-based diagnostic for COVID-19 with near single-copy sensitivity. The diagnostic performances of all three technology platforms were also compared. Our study aimed to provide more insights into the molecular detection of SARS-CoV-2, and also to present a novel diagnostic option for this new emerging virus. |
url |
https://doi.org/10.1371/journal.ppat.1008705 |
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