Ubiquity of <i>Euglena mutabilis</i> Population in Three Ecologically Distinct Acidic Habitats in Southwestern Japan

Ubiquity of <i>Euglena mutabilis</i> Population in Three Ecologically Distinct Acidic Habitats in Southwestern Japan

Three strains of <i>Euglena mutabilis</i> were isolated from sediments in acidic inland water systems (pH = 3.4–4.7), in Southwestern Japan—acid mine drainage in Sensui (Fukuoka), cold sulfidic spring in Bougatsuru (Oita), and a temporal pool in the Ebinokogen volcanic area (Miyazaki). A...

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Main Authors: Katsunori Yanagawa, Akira Haraguchi, Kai Yoshitake, Katsuhiro Asamatsu, Masanari Harano, Kei Yamashita, Jun-ichiro Ishibashi
Format: Article
Language:English
Published: MDPI AG 2021-06-01
Series:Water
Subjects:
acid mine drainage
benthic <i>Euglena</i>
volcanic spring
Online Access:https://www.mdpi.com/2073-4441/13/11/1570
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spelling doaj-58aa563ae7cb44c7930e95a76f72ec992021-06-30T23:04:31ZengMDPI AGWater2073-44412021-06-01131570157010.3390/w13111570Ubiquity of <i>Euglena mutabilis</i> Population in Three Ecologically Distinct Acidic Habitats in Southwestern JapanKatsunori Yanagawa0Akira Haraguchi1Kai Yoshitake2Katsuhiro Asamatsu3Masanari Harano4Kei Yamashita5Jun-ichiro Ishibashi6Faculty of Environmental Engineering, The University of Kitakyushu, Kitakyushu 808-0135, JapanFaculty of Environmental Engineering, The University of Kitakyushu, Kitakyushu 808-0135, JapanFaculty of Environmental Engineering, The University of Kitakyushu, Kitakyushu 808-0135, JapanFaculty of Environmental Engineering, The University of Kitakyushu, Kitakyushu 808-0135, JapanFaculty of Environmental Engineering, The University of Kitakyushu, Kitakyushu 808-0135, JapanDepartment of Earth and Planetary Sciences, Faculty of Science, Kyushu University, Fukuoka 819-0395, JapanDepartment of Earth and Planetary Sciences, Faculty of Science, Kyushu University, Fukuoka 819-0395, JapanThree strains of <i>Euglena mutabilis</i> were isolated from sediments in acidic inland water systems (pH = 3.4–4.7), in Southwestern Japan—acid mine drainage in Sensui (Fukuoka), cold sulfidic spring in Bougatsuru (Oita), and a temporal pool in the Ebinokogen volcanic area (Miyazaki). All strains grew well in acidic media at pH 3.07. Phylogenetic analysis among these three strains showed high similarities to plastid SSU and nuclear SSU rRNA gene sequences (99.86% and 99.76%, respectively). They were closely related to the cultured isolates from other highly acidic habitats (pH = 2.0–5.9). Concentration of sulfate, aluminum, calcium, and iron had 7–70 fold of differences among the three studied habitats. Our results imply that the rRNA genes of <i>E. mutabilis</i> have compensated for their low genetic diversity by adapting to a wide pH range, as well as various water chemistry of habitats.https://www.mdpi.com/2073-4441/13/11/1570acid mine drainagebenthic <i>Euglena</i>volcanic spring
collection DOAJ
language English
format Article
sources DOAJ
author Katsunori Yanagawa
Akira Haraguchi
Kai Yoshitake
Katsuhiro Asamatsu
Masanari Harano
Kei Yamashita
Jun-ichiro Ishibashi
spellingShingle Katsunori Yanagawa
Akira Haraguchi
Kai Yoshitake
Katsuhiro Asamatsu
Masanari Harano
Kei Yamashita
Jun-ichiro Ishibashi
Ubiquity of <i>Euglena mutabilis</i> Population in Three Ecologically Distinct Acidic Habitats in Southwestern Japan
Water
acid mine drainage
benthic <i>Euglena</i>
volcanic spring
author_facet Katsunori Yanagawa
Akira Haraguchi
Kai Yoshitake
Katsuhiro Asamatsu
Masanari Harano
Kei Yamashita
Jun-ichiro Ishibashi
author_sort Katsunori Yanagawa
title Ubiquity of <i>Euglena mutabilis</i> Population in Three Ecologically Distinct Acidic Habitats in Southwestern Japan
title_short Ubiquity of <i>Euglena mutabilis</i> Population in Three Ecologically Distinct Acidic Habitats in Southwestern Japan
title_full Ubiquity of <i>Euglena mutabilis</i> Population in Three Ecologically Distinct Acidic Habitats in Southwestern Japan
title_fullStr Ubiquity of <i>Euglena mutabilis</i> Population in Three Ecologically Distinct Acidic Habitats in Southwestern Japan
title_full_unstemmed Ubiquity of <i>Euglena mutabilis</i> Population in Three Ecologically Distinct Acidic Habitats in Southwestern Japan
title_sort ubiquity of <i>euglena mutabilis</i> population in three ecologically distinct acidic habitats in southwestern japan
publisher MDPI AG
series Water
issn 2073-4441
publishDate 2021-06-01
description Three strains of <i>Euglena mutabilis</i> were isolated from sediments in acidic inland water systems (pH = 3.4–4.7), in Southwestern Japan—acid mine drainage in Sensui (Fukuoka), cold sulfidic spring in Bougatsuru (Oita), and a temporal pool in the Ebinokogen volcanic area (Miyazaki). All strains grew well in acidic media at pH 3.07. Phylogenetic analysis among these three strains showed high similarities to plastid SSU and nuclear SSU rRNA gene sequences (99.86% and 99.76%, respectively). They were closely related to the cultured isolates from other highly acidic habitats (pH = 2.0–5.9). Concentration of sulfate, aluminum, calcium, and iron had 7–70 fold of differences among the three studied habitats. Our results imply that the rRNA genes of <i>E. mutabilis</i> have compensated for their low genetic diversity by adapting to a wide pH range, as well as various water chemistry of habitats.
topic acid mine drainage
benthic <i>Euglena</i>
volcanic spring
url https://www.mdpi.com/2073-4441/13/11/1570
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