An engineered micropattern to reduce bacterial colonization, platelet adhesion and fibrin sheath formation for improved biocompatibility of central venous catheters
Abstract BackgroundCatheter‐related bloodstream infections (CRBSIs) and catheter‐related thrombosis (CRT) are common complications of central venous catheters (CVC), which are used to monitor patient health and deliver medications. CVCs are subject to protein adsorption and platelet adhesion as well...
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doaj-58b9f56d5b8b47fb9e89afa8545e3a8a2020-11-25T02:50:37ZengWileyClinical and Translational Medicine2001-13262015-12-0141n/an/a10.1186/s40169-015-0050-9An engineered micropattern to reduce bacterial colonization, platelet adhesion and fibrin sheath formation for improved biocompatibility of central venous cathetersRhea M May0Chelsea M Magin1Ethan E Mann2Michael C Drinker3John C Fraser4Christopher A Siedlecki5Anthony B Brennan6Shravanthi T Reddy7Sharklet TechnologiesInc12635 E. Montview Blvd. Suite 155CO 80045AuroraCOUSASharklet TechnologiesInc12635 E. Montview Blvd. Suite 155CO 80045AuroraCOUSASharklet TechnologiesInc12635 E. Montview Blvd. Suite 155CO 80045AuroraCOUSASharklet TechnologiesInc12635 E. Montview Blvd. Suite 155CO 80045AuroraCOUSASharklet TechnologiesInc12635 E. Montview Blvd. Suite 155CO 80045AuroraCOUSADepartments of Bioengineering and SurgeryPennsylvania State UniversityHersheyPAUSADepartments of Materials Science and Engineering and Biomedical Engineering University of Florida32611GainesvilleFLUSASharklet TechnologiesInc12635 E. Montview Blvd. Suite 155CO 80045AuroraCOUSAAbstract BackgroundCatheter‐related bloodstream infections (CRBSIs) and catheter‐related thrombosis (CRT) are common complications of central venous catheters (CVC), which are used to monitor patient health and deliver medications. CVCs are subject to protein adsorption and platelet adhesion as well as colonization by the natural skin flora (i.e. Staphylococcus aureus and Staphylococcus epidermidis). Antimicrobial and antithrombotic drugs can prevent infections and thrombosis‐related complications, but have associated resistance and safety risks. Surface topographies have shown promise in limiting platelet and bacterial adhesion, so it was hypothesized that an engineered Sharklet micropattern, inspired by shark‐skin, may provide a combined approach as it has wide reaching anti‐fouling capabilities. To assess the feasibility for this micropattern to improve CVC‐related healthcare outcomes, bacterial colonization and platelet interactions were analyzed in vitro on a material common for vascular access devices. MethodsTo evaluate bacterial inhibition after simulated vascular exposure, micropatterned thermoplastic polyurethane surfaces were preconditioned with blood proteins in vitro then subjected to a bacterial challenge for 1 and 18 h. Platelet adhesion was assessed with fluorescent microscopy after incubation of the surfaces with platelet‐rich plasma (PRP) supplemented with calcium. Platelet activation was further assessed by monitoring fibrin formation with fluorescent microscopy after exposure of the surfaces to platelet‐rich plasma (PRP) supplemented with calcium in a flow‐cell. Results are reported as percent reductions and significance is based on t‐tests and ANOVA models of log reductions. All experiments were replicated at least three times. ResultsBlood and serum conditioned micropatterned surfaces reduced 18 h S. aureus and S. epidermidis colonization by 70% (p ≤ 0.05) and 71% (p < 0.01), respectively, when compared to preconditioned unpatterned controls. Additionally, platelet adhesion and fibrin sheath formation were reduced by 86% and 80% (p < 0.05), respectively, on the micropattern, when compared to controls. ConclusionsThe Sharklet micropattern, in a CVC‐relevant thermoplastic polyurethane, significantly reduced bacterial colonization and relevant platelet interactions after simulated vascular exposure. These results suggest that the incorporation of the Sharklet micropattern on the surface of a CVC may inhibit the initial events that lead to CRBSI and CRT.https://doi.org/10.1186/s40169-015-0050-9SharkletMicrotopographyPlatelet activationBlood compatibilityInfectionCRT |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rhea M May Chelsea M Magin Ethan E Mann Michael C Drinker John C Fraser Christopher A Siedlecki Anthony B Brennan Shravanthi T Reddy |
spellingShingle |
Rhea M May Chelsea M Magin Ethan E Mann Michael C Drinker John C Fraser Christopher A Siedlecki Anthony B Brennan Shravanthi T Reddy An engineered micropattern to reduce bacterial colonization, platelet adhesion and fibrin sheath formation for improved biocompatibility of central venous catheters Clinical and Translational Medicine Sharklet Microtopography Platelet activation Blood compatibility Infection CRT |
author_facet |
Rhea M May Chelsea M Magin Ethan E Mann Michael C Drinker John C Fraser Christopher A Siedlecki Anthony B Brennan Shravanthi T Reddy |
author_sort |
Rhea M May |
title |
An engineered micropattern to reduce bacterial colonization, platelet adhesion and fibrin sheath formation for improved biocompatibility of central venous catheters |
title_short |
An engineered micropattern to reduce bacterial colonization, platelet adhesion and fibrin sheath formation for improved biocompatibility of central venous catheters |
title_full |
An engineered micropattern to reduce bacterial colonization, platelet adhesion and fibrin sheath formation for improved biocompatibility of central venous catheters |
title_fullStr |
An engineered micropattern to reduce bacterial colonization, platelet adhesion and fibrin sheath formation for improved biocompatibility of central venous catheters |
title_full_unstemmed |
An engineered micropattern to reduce bacterial colonization, platelet adhesion and fibrin sheath formation for improved biocompatibility of central venous catheters |
title_sort |
engineered micropattern to reduce bacterial colonization, platelet adhesion and fibrin sheath formation for improved biocompatibility of central venous catheters |
publisher |
Wiley |
series |
Clinical and Translational Medicine |
issn |
2001-1326 |
publishDate |
2015-12-01 |
description |
Abstract BackgroundCatheter‐related bloodstream infections (CRBSIs) and catheter‐related thrombosis (CRT) are common complications of central venous catheters (CVC), which are used to monitor patient health and deliver medications. CVCs are subject to protein adsorption and platelet adhesion as well as colonization by the natural skin flora (i.e. Staphylococcus aureus and Staphylococcus epidermidis). Antimicrobial and antithrombotic drugs can prevent infections and thrombosis‐related complications, but have associated resistance and safety risks. Surface topographies have shown promise in limiting platelet and bacterial adhesion, so it was hypothesized that an engineered Sharklet micropattern, inspired by shark‐skin, may provide a combined approach as it has wide reaching anti‐fouling capabilities. To assess the feasibility for this micropattern to improve CVC‐related healthcare outcomes, bacterial colonization and platelet interactions were analyzed in vitro on a material common for vascular access devices. MethodsTo evaluate bacterial inhibition after simulated vascular exposure, micropatterned thermoplastic polyurethane surfaces were preconditioned with blood proteins in vitro then subjected to a bacterial challenge for 1 and 18 h. Platelet adhesion was assessed with fluorescent microscopy after incubation of the surfaces with platelet‐rich plasma (PRP) supplemented with calcium. Platelet activation was further assessed by monitoring fibrin formation with fluorescent microscopy after exposure of the surfaces to platelet‐rich plasma (PRP) supplemented with calcium in a flow‐cell. Results are reported as percent reductions and significance is based on t‐tests and ANOVA models of log reductions. All experiments were replicated at least three times. ResultsBlood and serum conditioned micropatterned surfaces reduced 18 h S. aureus and S. epidermidis colonization by 70% (p ≤ 0.05) and 71% (p < 0.01), respectively, when compared to preconditioned unpatterned controls. Additionally, platelet adhesion and fibrin sheath formation were reduced by 86% and 80% (p < 0.05), respectively, on the micropattern, when compared to controls. ConclusionsThe Sharklet micropattern, in a CVC‐relevant thermoplastic polyurethane, significantly reduced bacterial colonization and relevant platelet interactions after simulated vascular exposure. These results suggest that the incorporation of the Sharklet micropattern on the surface of a CVC may inhibit the initial events that lead to CRBSI and CRT. |
topic |
Sharklet Microtopography Platelet activation Blood compatibility Infection CRT |
url |
https://doi.org/10.1186/s40169-015-0050-9 |
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