ATP driven clathrin dependent entry of carbon nanospheres prefer cells with glucose receptors
<p>Abstract</p> <p>Background</p> <p>Intrinsically fluorescent glucose derived carbon nanospheres (CSP) efficiently enter mammalian cells and also cross the blood brain barrier (BBB). However, the mechanistic details of CSP entry inside mammalian cells and its specifici...
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doaj-58e9a20d88884af989c4825cb3a7d5772020-11-24T22:08:17ZengBMCJournal of Nanobiotechnology1477-31552012-08-011013510.1186/1477-3155-10-35ATP driven clathrin dependent entry of carbon nanospheres prefer cells with glucose receptorsSelvi Ruthrotha BChatterjee SnehajyotiJagadeesan DineshChaturbedy PiyushSuma BangaloreEswaramoorthy MuthusamyKundu Tapas K<p>Abstract</p> <p>Background</p> <p>Intrinsically fluorescent glucose derived carbon nanospheres (CSP) efficiently enter mammalian cells and also cross the blood brain barrier (BBB). However, the mechanistic details of CSP entry inside mammalian cells and its specificity are not known.</p> <p>Results</p> <p>In this report, the biochemical and cellular mechanism of CSP entry into the living cell have been investigated. By employing confocal imaging we show that CSP entry into the mammalian cells is an ATP-dependent clathrin mediated endocytosis process. Zeta potential studies suggest that it has a strong preference for cells which possess high levels of glucose transporters such as the glial cells, thereby enabling it to target individual organs/tissues such as the brain with increased specificity.</p> <p>Conclusion</p> <p>The endocytosis of Glucose derived CSP into mammalian cells is an ATP dependent process mediated by clathrin coated pits. CSPs utilize the surface functional groups to target cells containing glucose transporters on its membrane thereby implicating a potential application for specific targeting of the brain or cancer cells.</p> http://www.jnanobiotechnology.com/content/10/1/35 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Selvi Ruthrotha B Chatterjee Snehajyoti Jagadeesan Dinesh Chaturbedy Piyush Suma Bangalore Eswaramoorthy Muthusamy Kundu Tapas K |
spellingShingle |
Selvi Ruthrotha B Chatterjee Snehajyoti Jagadeesan Dinesh Chaturbedy Piyush Suma Bangalore Eswaramoorthy Muthusamy Kundu Tapas K ATP driven clathrin dependent entry of carbon nanospheres prefer cells with glucose receptors Journal of Nanobiotechnology |
author_facet |
Selvi Ruthrotha B Chatterjee Snehajyoti Jagadeesan Dinesh Chaturbedy Piyush Suma Bangalore Eswaramoorthy Muthusamy Kundu Tapas K |
author_sort |
Selvi Ruthrotha B |
title |
ATP driven clathrin dependent entry of carbon nanospheres prefer cells with glucose receptors |
title_short |
ATP driven clathrin dependent entry of carbon nanospheres prefer cells with glucose receptors |
title_full |
ATP driven clathrin dependent entry of carbon nanospheres prefer cells with glucose receptors |
title_fullStr |
ATP driven clathrin dependent entry of carbon nanospheres prefer cells with glucose receptors |
title_full_unstemmed |
ATP driven clathrin dependent entry of carbon nanospheres prefer cells with glucose receptors |
title_sort |
atp driven clathrin dependent entry of carbon nanospheres prefer cells with glucose receptors |
publisher |
BMC |
series |
Journal of Nanobiotechnology |
issn |
1477-3155 |
publishDate |
2012-08-01 |
description |
<p>Abstract</p> <p>Background</p> <p>Intrinsically fluorescent glucose derived carbon nanospheres (CSP) efficiently enter mammalian cells and also cross the blood brain barrier (BBB). However, the mechanistic details of CSP entry inside mammalian cells and its specificity are not known.</p> <p>Results</p> <p>In this report, the biochemical and cellular mechanism of CSP entry into the living cell have been investigated. By employing confocal imaging we show that CSP entry into the mammalian cells is an ATP-dependent clathrin mediated endocytosis process. Zeta potential studies suggest that it has a strong preference for cells which possess high levels of glucose transporters such as the glial cells, thereby enabling it to target individual organs/tissues such as the brain with increased specificity.</p> <p>Conclusion</p> <p>The endocytosis of Glucose derived CSP into mammalian cells is an ATP dependent process mediated by clathrin coated pits. CSPs utilize the surface functional groups to target cells containing glucose transporters on its membrane thereby implicating a potential application for specific targeting of the brain or cancer cells.</p> |
url |
http://www.jnanobiotechnology.com/content/10/1/35 |
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