Molecular characterisation and nucleotide sequence analysis of canine parvovirus strains in vaccines in India

• Main Authors: Sukdeb Nandi, Rajendra Anbazhagan, Manoj Kumar
• Format: Article
• Language: English
• Published: Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale 2010-03-01
• Series: Veterinaria Italiana
• Subjects: Canine parvovirus; DNA; Dog; Feline panleukopenia virus; Gastroenteritis; India; Parvovirus; PCR; Polymerase chain reaction; Sequencing; Vaccine; Virus.
• Online Access: http://www.izs.it/vet_italiana/2010/46_1/69.pdf

Canine parvovirus 2 (CPV‑2) is one of the most important viruses that causes haemorrhagic gastroenteritis and myocarditis of dogs worldwide. The picture has been complicated further due to the emergence of new mutants of CPV, namely: CPV‑2a, CPV‑2b and CPV‑2c. In this study, the molecular characterisation of strains present in the CPV vaccines available on the Indian market was performed using polymerase chain reaction and DNA sequencing. The VP1/VP2 genes of two vaccine strains and a field strain (Bhopal) were sequenced and the nucleotide and the deduced amino acid sequences were compared. The results indicated that the isolate belonged to CPV type 2b and the strains in the vaccines belonged to type CPV‑2. From the study, it is inferred that the CPV strain used in commercially available vaccine preparation differed from the strains present in CPV infection in dogs in India