Genistein From Fructus sophorae Protects Mice From Radiation-Induced Intestinal Injury
The development of an effective pharmacological countermeasure is needed to reduce the morbidity and mortality in high-dose ionizing radiation-induced acute damage. Genistein has shown bioactivity in alleviating radiation damage and is currently synthesized by chemosynthetic methods. Due to concerns...
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Format: | Article |
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Frontiers Media S.A.
2021-05-01
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Series: | Frontiers in Pharmacology |
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Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2021.655652/full |
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doaj-58ecb2d5fa594f4a9b92bbb5d6aea446 |
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record_format |
Article |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jieyu Zhang Jieyu Zhang Zhijun Pang Yuting Zhang Yuting Zhang Jiaxin Liu Jiaxin Liu Zhaowei Wang Chuanyang Xu Lei He Weina Li Kuo Zhang Wangqian Zhang Shuning Wang Cun Zhang Qiang Hao Yingqi Zhang Meng Li Zhengmin Li |
spellingShingle |
Jieyu Zhang Jieyu Zhang Zhijun Pang Yuting Zhang Yuting Zhang Jiaxin Liu Jiaxin Liu Zhaowei Wang Chuanyang Xu Lei He Weina Li Kuo Zhang Wangqian Zhang Shuning Wang Cun Zhang Qiang Hao Yingqi Zhang Meng Li Zhengmin Li Genistein From Fructus sophorae Protects Mice From Radiation-Induced Intestinal Injury Frontiers in Pharmacology Fructus sophorae X-ray irradiation iirradiation-induced intestinal injury active pharmaceutical ingredient genistein |
author_facet |
Jieyu Zhang Jieyu Zhang Zhijun Pang Yuting Zhang Yuting Zhang Jiaxin Liu Jiaxin Liu Zhaowei Wang Chuanyang Xu Lei He Weina Li Kuo Zhang Wangqian Zhang Shuning Wang Cun Zhang Qiang Hao Yingqi Zhang Meng Li Zhengmin Li |
author_sort |
Jieyu Zhang |
title |
Genistein From Fructus sophorae Protects Mice From Radiation-Induced Intestinal Injury |
title_short |
Genistein From Fructus sophorae Protects Mice From Radiation-Induced Intestinal Injury |
title_full |
Genistein From Fructus sophorae Protects Mice From Radiation-Induced Intestinal Injury |
title_fullStr |
Genistein From Fructus sophorae Protects Mice From Radiation-Induced Intestinal Injury |
title_full_unstemmed |
Genistein From Fructus sophorae Protects Mice From Radiation-Induced Intestinal Injury |
title_sort |
genistein from fructus sophorae protects mice from radiation-induced intestinal injury |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Pharmacology |
issn |
1663-9812 |
publishDate |
2021-05-01 |
description |
The development of an effective pharmacological countermeasure is needed to reduce the morbidity and mortality in high-dose ionizing radiation-induced acute damage. Genistein has shown bioactivity in alleviating radiation damage and is currently synthesized by chemosynthetic methods. Due to concerns about chemical residues and high costs, the clinical application of genistein is still a major challenge. In this study, we aimed to establish an efficient method for the extraction of genistein from Fructus sophorae. The effects of extracted genistein (FSGen) on preventing intestinal injury from radiation were further investigated in this study. C57/BL mice were exposed to 7.5 Gy whole body irradiation with and without FSGen treatments. Histological analysis demonstrated significant structural and functional restitution of the intestine and bone marrow in FSGen-pretreated cohorts after irradiation. Through mRNA expression, protein expression, and small interfering RNA analyses, we demonstrated that FSGen protects IEC-6 cells against radiation damage by upregulating the Rassf1a and Ercc1 genes to effectively attenuate DNA irradiation damage. Together, our data established an effective method to extract genistein from the Fructus sophorae plant with high purity, and validated the beneficial roles of the FSGen in protecting the radiation damage. These results promise the future applications of Fructus sophorae extracted genistein in the protection of radiation related damages. |
topic |
Fructus sophorae X-ray irradiation iirradiation-induced intestinal injury active pharmaceutical ingredient genistein |
url |
https://www.frontiersin.org/articles/10.3389/fphar.2021.655652/full |
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doaj-58ecb2d5fa594f4a9b92bbb5d6aea4462021-05-21T05:12:57ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122021-05-011210.3389/fphar.2021.655652655652Genistein From Fructus sophorae Protects Mice From Radiation-Induced Intestinal InjuryJieyu Zhang0Jieyu Zhang1Zhijun Pang2Yuting Zhang3Yuting Zhang4Jiaxin Liu5Jiaxin Liu6Zhaowei Wang7Chuanyang Xu8Lei He9Weina Li10Kuo Zhang11Wangqian Zhang12Shuning Wang13Cun Zhang14Qiang Hao15Yingqi Zhang16Meng Li17Zhengmin Li18The State Key Laboratory of Cancer Biology, Department of Biopharmaceutics, School of Pharmacy, Fourth Military Medical University, Xi’an, ChinaDepartment of Dermatology, Xijing Hospital, Fourth Military Medical University, Xi’an, ChinaDepartment of Laboratory Medicine, The 971th Naval Hospital, Shandong, ChinaThe State Key Laboratory of Cancer Biology, Department of Biopharmaceutics, School of Pharmacy, Fourth Military Medical University, Xi’an, ChinaSecond Battalion of Basic Medical College, Fourth Military Medical University, Xi’an, ChinaThe State Key Laboratory of Cancer Biology, Department of Biopharmaceutics, School of Pharmacy, Fourth Military Medical University, Xi’an, ChinaSecond Battalion of Basic Medical College, Fourth Military Medical University, Xi’an, ChinaThe State Key Laboratory of Cancer Biology, Department of Biopharmaceutics, School of Pharmacy, Fourth Military Medical University, Xi’an, ChinaThe State Key Laboratory of Cancer Biology, Department of Biopharmaceutics, School of Pharmacy, Fourth Military Medical University, Xi’an, ChinaThe State Key Laboratory of Cancer Biology, Department of Biopharmaceutics, School of Pharmacy, Fourth Military Medical University, Xi’an, ChinaThe State Key Laboratory of Cancer Biology, Department of Biopharmaceutics, School of Pharmacy, Fourth Military Medical University, Xi’an, ChinaThe State Key Laboratory of Cancer Biology, Department of Biopharmaceutics, School of Pharmacy, Fourth Military Medical University, Xi’an, ChinaThe State Key Laboratory of Cancer Biology, Department of Biopharmaceutics, School of Pharmacy, Fourth Military Medical University, Xi’an, ChinaThe State Key Laboratory of Cancer Biology, Department of Biopharmaceutics, School of Pharmacy, Fourth Military Medical University, Xi’an, ChinaThe State Key Laboratory of Cancer Biology, Department of Biopharmaceutics, School of Pharmacy, Fourth Military Medical University, Xi’an, ChinaThe State Key Laboratory of Cancer Biology, Department of Biopharmaceutics, School of Pharmacy, Fourth Military Medical University, Xi’an, ChinaThe State Key Laboratory of Cancer Biology, Department of Biopharmaceutics, School of Pharmacy, Fourth Military Medical University, Xi’an, ChinaThe State Key Laboratory of Cancer Biology, Department of Biopharmaceutics, School of Pharmacy, Fourth Military Medical University, Xi’an, ChinaDepartment of Anesthesiology, Tangdu Hospital, Fourth Military Medical University, Xi’an, ChinaThe development of an effective pharmacological countermeasure is needed to reduce the morbidity and mortality in high-dose ionizing radiation-induced acute damage. Genistein has shown bioactivity in alleviating radiation damage and is currently synthesized by chemosynthetic methods. Due to concerns about chemical residues and high costs, the clinical application of genistein is still a major challenge. In this study, we aimed to establish an efficient method for the extraction of genistein from Fructus sophorae. The effects of extracted genistein (FSGen) on preventing intestinal injury from radiation were further investigated in this study. C57/BL mice were exposed to 7.5 Gy whole body irradiation with and without FSGen treatments. Histological analysis demonstrated significant structural and functional restitution of the intestine and bone marrow in FSGen-pretreated cohorts after irradiation. Through mRNA expression, protein expression, and small interfering RNA analyses, we demonstrated that FSGen protects IEC-6 cells against radiation damage by upregulating the Rassf1a and Ercc1 genes to effectively attenuate DNA irradiation damage. Together, our data established an effective method to extract genistein from the Fructus sophorae plant with high purity, and validated the beneficial roles of the FSGen in protecting the radiation damage. These results promise the future applications of Fructus sophorae extracted genistein in the protection of radiation related damages.https://www.frontiersin.org/articles/10.3389/fphar.2021.655652/fullFructus sophoraeX-ray irradiationiirradiation-induced intestinal injuryactive pharmaceutical ingredientgenistein |