Generation of two isogenic knockout PKD2 iPS cell lines, IRFMNi003-A-1 and IRFMNi003-A-2, using CRISPR/Cas9 technology

Generation of two isogenic knockout PKD2 iPS cell lines, IRFMNi003-A-1 and IRFMNi003-A-2, using CRISPR/Cas9 technology

Autosomal dominant polycystic kidney disease (ADPKD) is the most prevalent inherited renal disease, characterized by multiple cysts that can lead to kidney failure resulting in end-stage renal disease. ADPKD is mainly caused by mutations in either the PKD1 and PKD2 genes, encoding for polycystin-1 a...

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Main Authors: Piera Trionfini, Osele Ciampi, Elena Romano, Ariela Benigni, Susanna Tomasoni
Format: Article
Language:English
Published: Elsevier 2020-01-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506119302971
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spelling doaj-58ed6469b4ac464d870a597e20d670902020-11-24T23:49:37ZengElsevierStem Cell Research1873-50612020-01-0142Generation of two isogenic knockout PKD2 iPS cell lines, IRFMNi003-A-1 and IRFMNi003-A-2, using CRISPR/Cas9 technologyPiera Trionfini0Osele Ciampi1Elena Romano2Ariela Benigni3Susanna Tomasoni4Istituto di Ricerche Farmacologiche Mario Negri IRCCS, Centro Anna Maria Astori, Science and Technology Park Kilometro Rosso, Via Stezzano, 87, 24126 Bergamo, ItalyIstituto di Ricerche Farmacologiche Mario Negri IRCCS, Centro Anna Maria Astori, Science and Technology Park Kilometro Rosso, Via Stezzano, 87, 24126 Bergamo, ItalyIstituto di Ricerche Farmacologiche Mario Negri IRCCS, Centro Anna Maria Astori, Science and Technology Park Kilometro Rosso, Via Stezzano, 87, 24126 Bergamo, ItalyIstituto di Ricerche Farmacologiche Mario Negri IRCCS, Centro Anna Maria Astori, Science and Technology Park Kilometro Rosso, Via Stezzano, 87, 24126 Bergamo, ItalyCorresponding author.; Istituto di Ricerche Farmacologiche Mario Negri IRCCS, Centro Anna Maria Astori, Science and Technology Park Kilometro Rosso, Via Stezzano, 87, 24126 Bergamo, ItalyAutosomal dominant polycystic kidney disease (ADPKD) is the most prevalent inherited renal disease, characterized by multiple cysts that can lead to kidney failure resulting in end-stage renal disease. ADPKD is mainly caused by mutations in either the PKD1 and PKD2 genes, encoding for polycystin-1 and polycystin-2, respectively. In order to clarify the disease mechanisms, here we describe the generation of two isogenic induced pluripotent stem cell (iPSC) lines in which the PKD2 gene was deleted using CRISPR/Cas9 technology. The PKD2−/− iPSCs expressed the main pluripotency markers, were able to differentiate into the three germ layers and had a normal karyotype.http://www.sciencedirect.com/science/article/pii/S1873506119302971
collection DOAJ
language English
format Article
sources DOAJ
author Piera Trionfini
Osele Ciampi
Elena Romano
Ariela Benigni
Susanna Tomasoni
spellingShingle Piera Trionfini
Osele Ciampi
Elena Romano
Ariela Benigni
Susanna Tomasoni
Generation of two isogenic knockout PKD2 iPS cell lines, IRFMNi003-A-1 and IRFMNi003-A-2, using CRISPR/Cas9 technology
Stem Cell Research
author_facet Piera Trionfini
Osele Ciampi
Elena Romano
Ariela Benigni
Susanna Tomasoni
author_sort Piera Trionfini
title Generation of two isogenic knockout PKD2 iPS cell lines, IRFMNi003-A-1 and IRFMNi003-A-2, using CRISPR/Cas9 technology
title_short Generation of two isogenic knockout PKD2 iPS cell lines, IRFMNi003-A-1 and IRFMNi003-A-2, using CRISPR/Cas9 technology
title_full Generation of two isogenic knockout PKD2 iPS cell lines, IRFMNi003-A-1 and IRFMNi003-A-2, using CRISPR/Cas9 technology
title_fullStr Generation of two isogenic knockout PKD2 iPS cell lines, IRFMNi003-A-1 and IRFMNi003-A-2, using CRISPR/Cas9 technology
title_full_unstemmed Generation of two isogenic knockout PKD2 iPS cell lines, IRFMNi003-A-1 and IRFMNi003-A-2, using CRISPR/Cas9 technology
title_sort generation of two isogenic knockout pkd2 ips cell lines, irfmni003-a-1 and irfmni003-a-2, using crispr/cas9 technology
publisher Elsevier
series Stem Cell Research
issn 1873-5061
publishDate 2020-01-01
description Autosomal dominant polycystic kidney disease (ADPKD) is the most prevalent inherited renal disease, characterized by multiple cysts that can lead to kidney failure resulting in end-stage renal disease. ADPKD is mainly caused by mutations in either the PKD1 and PKD2 genes, encoding for polycystin-1 and polycystin-2, respectively. In order to clarify the disease mechanisms, here we describe the generation of two isogenic induced pluripotent stem cell (iPSC) lines in which the PKD2 gene was deleted using CRISPR/Cas9 technology. The PKD2−/− iPSCs expressed the main pluripotency markers, were able to differentiate into the three germ layers and had a normal karyotype.
url http://www.sciencedirect.com/science/article/pii/S1873506119302971
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