Cloning of Acyl-ACP Thioesterase FatA from Arachis hypogaea L. and Its Expression in Escherichia coli

In this study, a full-length cDNA of the acyl-ACP thioesterase, AhFatA, was cloned from developing seeds of Arachis hypogaea L. by 3′-RACE. Sequence analysis showed that the open reading frame encodes a peptide of 372 amino acids and has 50–70% identity with FatA from other plants. Real-time quantit...

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Bibliographic Details
Main Authors: Gao Chen, Zhen-ying Peng, Lei Shan, Ning Xuan, Gui-ying Tang, Yan Zhang, Lan Li, Qing-fang He, Yu-ping Bi
Format: Article
Language:English
Published: Hindawi Limited 2012-01-01
Series:Journal of Biomedicine and Biotechnology
Online Access:http://dx.doi.org/10.1155/2012/652579